Redefining and delineating pristine conditions: A methodological framework with an application to a minimally impacted Mediterranean stream basin

Pristine river ecosystems serve as critical reference points for understanding natural hydrochemical processes and nutrient cycling, providing valuable benchmarks for water quality assessment and conservation. However, globally, truly undisturbed rivers are rare, and the definition of “pristine” rem...

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Main Authors: Nikolaos Th. Skoulikidis, Leonidas Vardakas, Anastasia Lampou, Yiorgos Amaxidis, Sofia Laschou
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Ecological Indicators
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Online Access:http://www.sciencedirect.com/science/article/pii/S1470160X25010039
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author Nikolaos Th. Skoulikidis
Leonidas Vardakas
Anastasia Lampou
Yiorgos Amaxidis
Sofia Laschou
author_facet Nikolaos Th. Skoulikidis
Leonidas Vardakas
Anastasia Lampou
Yiorgos Amaxidis
Sofia Laschou
author_sort Nikolaos Th. Skoulikidis
collection DOAJ
description Pristine river ecosystems serve as critical reference points for understanding natural hydrochemical processes and nutrient cycling, providing valuable benchmarks for water quality assessment and conservation. However, globally, truly undisturbed rivers are rare, and the definition of “pristine” remains ambiguous. Addressing this gap, the present study proposes a comprehensive methodological framework for the classification of pristine rivers. The framework integrates as indicators exclusion criteria for anthropogenic pressures, morphological criteria, physicochemical quality indices, nutrient dynamics, and pristine nutrient threshold definitions. The framework was applied to the Sarandaporos River, one of the few remaining free-flowing and minimally impacted catchments in the Mediterranean region. During the summer baseflow period of 2024, data were collected from 37 sites, including in-situ physicochemical measurements and water samples analyzed for nutrients and major ions. Findings reveal that the river’s ecological integrity, based on hydromorphological and hydrochemical dynamics, remain largely intact, driven primarily by natural processes. These conditions result in exceptionally low nutrient concentrations. Applying the proposed framework, 18 sites, and their sub-basins –encompassing 36 % of the river basin– were classified as pristine. The proposed methodology offers a transferable and standardized approach for identifying pristine riverine conditions, emphasizing the importance of conserving natural ecosystems. This work contributes to the global discourse on aquatic ecosystem integrity and supports more informed water resource management and conservation practices.
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spelling doaj-art-6f72ae16bd8b42d0a50a3f7efc17da822025-08-25T04:14:10ZengElsevierEcological Indicators1470-160X2025-09-0117811407110.1016/j.ecolind.2025.114071Redefining and delineating pristine conditions: A methodological framework with an application to a minimally impacted Mediterranean stream basinNikolaos Th. Skoulikidis0Leonidas Vardakas1Anastasia Lampou2Yiorgos Amaxidis3Sofia Laschou4Corresponding author.; Hellenic Centre for Marine Research, Institute of Marine Biological Resources and Inland Waters, Anavyssos, GreeceHellenic Centre for Marine Research, Institute of Marine Biological Resources and Inland Waters, Anavyssos, GreeceHellenic Centre for Marine Research, Institute of Marine Biological Resources and Inland Waters, Anavyssos, GreeceHellenic Centre for Marine Research, Institute of Marine Biological Resources and Inland Waters, Anavyssos, GreeceHellenic Centre for Marine Research, Institute of Marine Biological Resources and Inland Waters, Anavyssos, GreecePristine river ecosystems serve as critical reference points for understanding natural hydrochemical processes and nutrient cycling, providing valuable benchmarks for water quality assessment and conservation. However, globally, truly undisturbed rivers are rare, and the definition of “pristine” remains ambiguous. Addressing this gap, the present study proposes a comprehensive methodological framework for the classification of pristine rivers. The framework integrates as indicators exclusion criteria for anthropogenic pressures, morphological criteria, physicochemical quality indices, nutrient dynamics, and pristine nutrient threshold definitions. The framework was applied to the Sarandaporos River, one of the few remaining free-flowing and minimally impacted catchments in the Mediterranean region. During the summer baseflow period of 2024, data were collected from 37 sites, including in-situ physicochemical measurements and water samples analyzed for nutrients and major ions. Findings reveal that the river’s ecological integrity, based on hydromorphological and hydrochemical dynamics, remain largely intact, driven primarily by natural processes. These conditions result in exceptionally low nutrient concentrations. Applying the proposed framework, 18 sites, and their sub-basins –encompassing 36 % of the river basin– were classified as pristine. The proposed methodology offers a transferable and standardized approach for identifying pristine riverine conditions, emphasizing the importance of conserving natural ecosystems. This work contributes to the global discourse on aquatic ecosystem integrity and supports more informed water resource management and conservation practices.http://www.sciencedirect.com/science/article/pii/S1470160X25010039Water qualityHydromorphologyIndicatorsPressuresNutrientsProcesses
spellingShingle Nikolaos Th. Skoulikidis
Leonidas Vardakas
Anastasia Lampou
Yiorgos Amaxidis
Sofia Laschou
Redefining and delineating pristine conditions: A methodological framework with an application to a minimally impacted Mediterranean stream basin
Ecological Indicators
Water quality
Hydromorphology
Indicators
Pressures
Nutrients
Processes
title Redefining and delineating pristine conditions: A methodological framework with an application to a minimally impacted Mediterranean stream basin
title_full Redefining and delineating pristine conditions: A methodological framework with an application to a minimally impacted Mediterranean stream basin
title_fullStr Redefining and delineating pristine conditions: A methodological framework with an application to a minimally impacted Mediterranean stream basin
title_full_unstemmed Redefining and delineating pristine conditions: A methodological framework with an application to a minimally impacted Mediterranean stream basin
title_short Redefining and delineating pristine conditions: A methodological framework with an application to a minimally impacted Mediterranean stream basin
title_sort redefining and delineating pristine conditions a methodological framework with an application to a minimally impacted mediterranean stream basin
topic Water quality
Hydromorphology
Indicators
Pressures
Nutrients
Processes
url http://www.sciencedirect.com/science/article/pii/S1470160X25010039
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