Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway
Objective To investigate the effect of arbutin (Arb) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) under inflammatory conditions and the mechanism of NF-κB signaling pathway in this process. Methods The effects of Arb on the proliferation and osteogenic differentia...
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Editorial Office of Stomatology
2025-05-01
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| Series: | Kouqiang yixue |
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| Online Access: | https://www.stomatology.cn/fileup/1003-9872/PDF/1747804529884-1876521561.pdf |
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| author | GAO Jingping, JIA Linglu, LIANG Hongning, WEN Yong |
| author_facet | GAO Jingping, JIA Linglu, LIANG Hongning, WEN Yong |
| author_sort | GAO Jingping, JIA Linglu, LIANG Hongning, WEN Yong |
| collection | DOAJ |
| description | Objective To investigate the effect of arbutin (Arb) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) under inflammatory conditions and the mechanism of NF-κB signaling pathway in this process. Methods The effects of Arb on the proliferation and osteogenic differentiation of hPDLSCs were analyzed by CCK-8, alkaline phosphatase staining, Alizarin Red staining, and Western blot. After establishing an inflammatory model using lipopolysaccharide (LPS), the effect of Arb on the expression levels of inflammatory factors in hPDLSCs was analyzed by RT-qPCR. The effect of Arb on osteogenic differentiation of hPDLSCs was analyzed, and the effect of Arb on the NF-κB pathway was analyzed by Western blot. After adding the NF-κB signaling pathway activator PMA to the culture system, whether the effect of Arb on hPDLSCs changed was analyzed. Results 100 nmol/L Arb did not affect the proliferation of hPDLSCs, but significantly promoted cell osteogenic differentiation and inhibited the expression of inflammatory factors under LPS stimulation. Arb reduced the activation effect of LPS on the NF-κB signaling pathway and the inhibitory effect on cell osteogenic differentiation, while the efficacy of Arb was partially eliminated by PMA. Conclusion Arb alleviates the inhibitory effect of LPS on osteogenic differentiation of hPDLSCs by inhibiting NF-κB signaling. |
| format | Article |
| id | doaj-art-6f729d47e772439191ea4f5902547e3b |
| institution | DOAJ |
| issn | 1003-9872 |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Editorial Office of Stomatology |
| record_format | Article |
| series | Kouqiang yixue |
| spelling | doaj-art-6f729d47e772439191ea4f5902547e3b2025-08-20T03:17:31ZzhoEditorial Office of StomatologyKouqiang yixue1003-98722025-05-0145534735410.13591/j.cnki.kqyx.2025.05.005Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathwayGAO Jingping, JIA Linglu, LIANG Hongning, WEN Yong0Department of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan 250012, ChinaObjective To investigate the effect of arbutin (Arb) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) under inflammatory conditions and the mechanism of NF-κB signaling pathway in this process. Methods The effects of Arb on the proliferation and osteogenic differentiation of hPDLSCs were analyzed by CCK-8, alkaline phosphatase staining, Alizarin Red staining, and Western blot. After establishing an inflammatory model using lipopolysaccharide (LPS), the effect of Arb on the expression levels of inflammatory factors in hPDLSCs was analyzed by RT-qPCR. The effect of Arb on osteogenic differentiation of hPDLSCs was analyzed, and the effect of Arb on the NF-κB pathway was analyzed by Western blot. After adding the NF-κB signaling pathway activator PMA to the culture system, whether the effect of Arb on hPDLSCs changed was analyzed. Results 100 nmol/L Arb did not affect the proliferation of hPDLSCs, but significantly promoted cell osteogenic differentiation and inhibited the expression of inflammatory factors under LPS stimulation. Arb reduced the activation effect of LPS on the NF-κB signaling pathway and the inhibitory effect on cell osteogenic differentiation, while the efficacy of Arb was partially eliminated by PMA. Conclusion Arb alleviates the inhibitory effect of LPS on osteogenic differentiation of hPDLSCs by inhibiting NF-κB signaling.https://www.stomatology.cn/fileup/1003-9872/PDF/1747804529884-1876521561.pdf|periodontal ligament stem cells|arbutin|periodontitis|osteogenesis|nf-κb |
| spellingShingle | GAO Jingping, JIA Linglu, LIANG Hongning, WEN Yong Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway Kouqiang yixue |periodontal ligament stem cells|arbutin|periodontitis|osteogenesis|nf-κb |
| title | Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway |
| title_full | Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway |
| title_fullStr | Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway |
| title_full_unstemmed | Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway |
| title_short | Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway |
| title_sort | arbutin alleviates the inhibitory effect of lps on osteogenic differentiation of human periodontal ligament stem cells through the nf κb signaling pathway |
| topic | |periodontal ligament stem cells|arbutin|periodontitis|osteogenesis|nf-κb |
| url | https://www.stomatology.cn/fileup/1003-9872/PDF/1747804529884-1876521561.pdf |
| work_keys_str_mv | AT gaojingpingjialinglulianghongningwenyong arbutinalleviatestheinhibitoryeffectoflpsonosteogenicdifferentiationofhumanperiodontalligamentstemcellsthroughthenfkbsignalingpathway |