ANO1: central role and clinical significance in non-neoplastic and neoplastic diseases

Anoctamin 1 (ANO1), also known as TMEM16A, is a multifunctional protein that serves as a calcium-activated chloride channel (CaCC). It is ubiquitously expressed across various tissues, including epithelial cells, smooth muscle cells, and neurons, where it is integral to physiological processes such...

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Main Authors: Yanghao Hu, Yifei Zhang, Jiali He, Huihuang Rao, Duomi Zhang, Zhisen Shen, Chongchang Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Immunology
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Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1570333/full
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author Yanghao Hu
Yanghao Hu
Yifei Zhang
Yifei Zhang
Jiali He
Jiali He
Huihuang Rao
Huihuang Rao
Duomi Zhang
Zhisen Shen
Chongchang Zhou
author_facet Yanghao Hu
Yanghao Hu
Yifei Zhang
Yifei Zhang
Jiali He
Jiali He
Huihuang Rao
Huihuang Rao
Duomi Zhang
Zhisen Shen
Chongchang Zhou
author_sort Yanghao Hu
collection DOAJ
description Anoctamin 1 (ANO1), also known as TMEM16A, is a multifunctional protein that serves as a calcium-activated chloride channel (CaCC). It is ubiquitously expressed across various tissues, including epithelial cells, smooth muscle cells, and neurons, where it is integral to physiological processes such as epithelial secretion, smooth muscle contraction, neural conduction, and cell proliferation and migration. Dysregulation of ANO1 has been linked to the pathogenesis of numerous diseases. Extensive research has established its involvement in non-neoplastic conditions such as asthma, hypertension, and gastrointestinal (GI) dysfunction. Moreover, ANO1 has garnered significant attention for its role in the development and progression of cancers, including head and neck cancer, breast cancer, and lung cancer, where its overexpression correlates with increased tumor growth, metastasis, and poor prognosis. Additionally, ANO1 regulates multiple signaling pathways, including the epidermal growth factor receptor (EGFR) pathway, the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway, and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway, among others. These pathways are pivotal in regulating cell proliferation, migration, and invasion. Given its central role in these processes, ANO1 has emerged as a promising diagnostic biomarker and therapeutic target. Recent advancements in ANO1 research have highlighted its potential in disease diagnosis and treatment. Strategies targeting ANO1, such as small molecule modulators or gene-silencing techniques, have shown preclinical promise in both non-neoplastic and neoplastic diseases. This review explores the latest findings in ANO1 research, focusing on its mechanistic involvement in disease progression, its regulation, and its therapeutic potential. Modulating ANO1 activity may offer novel therapeutic strategies for effectively treating ANO1-associated diseases.
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spelling doaj-art-6f55037f462943dea7ee09f449ba3f452025-08-20T03:53:45ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.15703331570333ANO1: central role and clinical significance in non-neoplastic and neoplastic diseasesYanghao Hu0Yanghao Hu1Yifei Zhang2Yifei Zhang3Jiali He4Jiali He5Huihuang Rao6Huihuang Rao7Duomi Zhang8Zhisen Shen9Chongchang Zhou10Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, Zhejiang, ChinaHealth Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, Zhejiang, ChinaHealth Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, Zhejiang, ChinaHealth Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, Zhejiang, ChinaHealth Science Center, Ningbo University, Ningbo, Zhejiang, ChinaHealth Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, The Affiliated Lihuili Hospital of Ningbo University, Ningbo, Zhejiang, ChinaAnoctamin 1 (ANO1), also known as TMEM16A, is a multifunctional protein that serves as a calcium-activated chloride channel (CaCC). It is ubiquitously expressed across various tissues, including epithelial cells, smooth muscle cells, and neurons, where it is integral to physiological processes such as epithelial secretion, smooth muscle contraction, neural conduction, and cell proliferation and migration. Dysregulation of ANO1 has been linked to the pathogenesis of numerous diseases. Extensive research has established its involvement in non-neoplastic conditions such as asthma, hypertension, and gastrointestinal (GI) dysfunction. Moreover, ANO1 has garnered significant attention for its role in the development and progression of cancers, including head and neck cancer, breast cancer, and lung cancer, where its overexpression correlates with increased tumor growth, metastasis, and poor prognosis. Additionally, ANO1 regulates multiple signaling pathways, including the epidermal growth factor receptor (EGFR) pathway, the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway, and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway, among others. These pathways are pivotal in regulating cell proliferation, migration, and invasion. Given its central role in these processes, ANO1 has emerged as a promising diagnostic biomarker and therapeutic target. Recent advancements in ANO1 research have highlighted its potential in disease diagnosis and treatment. Strategies targeting ANO1, such as small molecule modulators or gene-silencing techniques, have shown preclinical promise in both non-neoplastic and neoplastic diseases. This review explores the latest findings in ANO1 research, focusing on its mechanistic involvement in disease progression, its regulation, and its therapeutic potential. Modulating ANO1 activity may offer novel therapeutic strategies for effectively treating ANO1-associated diseases.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1570333/fullANO1CaCCdiagnostic and therapeutic targetsignaling pathwaysbiomarker
spellingShingle Yanghao Hu
Yanghao Hu
Yifei Zhang
Yifei Zhang
Jiali He
Jiali He
Huihuang Rao
Huihuang Rao
Duomi Zhang
Zhisen Shen
Chongchang Zhou
ANO1: central role and clinical significance in non-neoplastic and neoplastic diseases
Frontiers in Immunology
ANO1
CaCC
diagnostic and therapeutic target
signaling pathways
biomarker
title ANO1: central role and clinical significance in non-neoplastic and neoplastic diseases
title_full ANO1: central role and clinical significance in non-neoplastic and neoplastic diseases
title_fullStr ANO1: central role and clinical significance in non-neoplastic and neoplastic diseases
title_full_unstemmed ANO1: central role and clinical significance in non-neoplastic and neoplastic diseases
title_short ANO1: central role and clinical significance in non-neoplastic and neoplastic diseases
title_sort ano1 central role and clinical significance in non neoplastic and neoplastic diseases
topic ANO1
CaCC
diagnostic and therapeutic target
signaling pathways
biomarker
url https://www.frontiersin.org/articles/10.3389/fimmu.2025.1570333/full
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