MUSTN1 and FABP3 interact to regulate adipogenesis and lipid deposition

Lipid deposition is related to agricultural animal production and human health, and elucidating its molecular regulatory mechanisms is a topic of interest and a challenge in current scientific research. Musculoskeletal embryonic nuclear protein 1 (MUSTN1) regulates growth and development, including...

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Main Authors: Yu Fu, Xin Hao, Jingru Nie, Hongliang Zhang, Peng Shang, Bo Zhang, Hao Zhang
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227525000641
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author Yu Fu
Xin Hao
Jingru Nie
Hongliang Zhang
Peng Shang
Bo Zhang
Hao Zhang
author_facet Yu Fu
Xin Hao
Jingru Nie
Hongliang Zhang
Peng Shang
Bo Zhang
Hao Zhang
author_sort Yu Fu
collection DOAJ
description Lipid deposition is related to agricultural animal production and human health, and elucidating its molecular regulatory mechanisms is a topic of interest and a challenge in current scientific research. Musculoskeletal embryonic nuclear protein 1 (MUSTN1) regulates growth and development, including muscle tissue; however, its role in fat deposition remains unknown. Thus, our objective was to determine this role. Our new findings were as follows: MUSTN1 was highly expressed in the fat tissue of pigs with strong adipose deposition capacity; functionally, MUSTN1 promoted the proliferation and adipogenic differentiation of porcine and mouse preadipocytes. MUSTN1 knockout mice were protected against HFD-induced obesity, hepatic steatosis, and insulin resistance; and fatty acid binding protein 3 was identified as an interacting protein of MUSTN1, which facilitated preadipocyte proliferation and differentiation by activating the phosphatidylinositol 3 kinase/AKT signaling pathways. This study reveals a positive regulator for fat development, which suggests a novel approach for studying obesity and animal genetic improvement through the modulation of MUSTN1 expression.
format Article
id doaj-art-6f49d434dcd444448d70adeefd46d1a8
institution Kabale University
issn 0022-2275
language English
publishDate 2025-05-01
publisher Elsevier
record_format Article
series Journal of Lipid Research
spelling doaj-art-6f49d434dcd444448d70adeefd46d1a82025-08-20T03:52:46ZengElsevierJournal of Lipid Research0022-22752025-05-0166510080410.1016/j.jlr.2025.100804MUSTN1 and FABP3 interact to regulate adipogenesis and lipid depositionYu Fu0Xin Hao1Jingru Nie2Hongliang Zhang3Peng Shang4Bo Zhang5Hao Zhang6State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China; Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Hainan, China; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China; Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Hainan, China; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaCollege of Animal Science, Xizang Agricultural and Animal Husbandry College, Linzhi, ChinaCollege of Animal Science, Xizang Agricultural and Animal Husbandry College, Linzhi, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China; For correspondence: Hao ZhangLipid deposition is related to agricultural animal production and human health, and elucidating its molecular regulatory mechanisms is a topic of interest and a challenge in current scientific research. Musculoskeletal embryonic nuclear protein 1 (MUSTN1) regulates growth and development, including muscle tissue; however, its role in fat deposition remains unknown. Thus, our objective was to determine this role. Our new findings were as follows: MUSTN1 was highly expressed in the fat tissue of pigs with strong adipose deposition capacity; functionally, MUSTN1 promoted the proliferation and adipogenic differentiation of porcine and mouse preadipocytes. MUSTN1 knockout mice were protected against HFD-induced obesity, hepatic steatosis, and insulin resistance; and fatty acid binding protein 3 was identified as an interacting protein of MUSTN1, which facilitated preadipocyte proliferation and differentiation by activating the phosphatidylinositol 3 kinase/AKT signaling pathways. This study reveals a positive regulator for fat development, which suggests a novel approach for studying obesity and animal genetic improvement through the modulation of MUSTN1 expression.http://www.sciencedirect.com/science/article/pii/S0022227525000641MUSTN1FABP3adipogenesislipid depositionPI3K/AKT
spellingShingle Yu Fu
Xin Hao
Jingru Nie
Hongliang Zhang
Peng Shang
Bo Zhang
Hao Zhang
MUSTN1 and FABP3 interact to regulate adipogenesis and lipid deposition
Journal of Lipid Research
MUSTN1
FABP3
adipogenesis
lipid deposition
PI3K/AKT
title MUSTN1 and FABP3 interact to regulate adipogenesis and lipid deposition
title_full MUSTN1 and FABP3 interact to regulate adipogenesis and lipid deposition
title_fullStr MUSTN1 and FABP3 interact to regulate adipogenesis and lipid deposition
title_full_unstemmed MUSTN1 and FABP3 interact to regulate adipogenesis and lipid deposition
title_short MUSTN1 and FABP3 interact to regulate adipogenesis and lipid deposition
title_sort mustn1 and fabp3 interact to regulate adipogenesis and lipid deposition
topic MUSTN1
FABP3
adipogenesis
lipid deposition
PI3K/AKT
url http://www.sciencedirect.com/science/article/pii/S0022227525000641
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