A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink
Upon bioprinting, cells are mixed with a biomaterial to fabricate a living tissue, thus emphasizing the importance of biomaterials. The biomaterial used in this study was a bio-ink prepared using skin decellularized extracellular matrix (dECM). Skin dECM was extracted by treating the dermis with che...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1575842 |
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| author | Joo-Yun Won Mi-Hee Lee Mi-Jeong Kim Kyung-Hyun Min Geunseon Ahn Ji-Seok Han Songwan Jin Won-Soo Yun Jin-Hyung Shim |
| author_facet | Joo-Yun Won Mi-Hee Lee Mi-Jeong Kim Kyung-Hyun Min Geunseon Ahn Ji-Seok Han Songwan Jin Won-Soo Yun Jin-Hyung Shim |
| author_sort | Joo-Yun Won |
| collection | DOAJ |
| description | Upon bioprinting, cells are mixed with a biomaterial to fabricate a living tissue, thus emphasizing the importance of biomaterials. The biomaterial used in this study was a bio-ink prepared using skin decellularized extracellular matrix (dECM). Skin dECM was extracted by treating the dermis with chemicals and enzymes; the basic structural and functional proteins of the ECM, including collagen, glycosaminoglycans (GAGs), bioreactive materials and growth factors, were preserved, whereas the resident cells that might cause immune rejection or inflammatory responses were removed. The bio-ink based on dECM powder, together with human dermal fibroblasts (HDFs), was loaded into the nozzle of the 3D bioprinter to create the 3D construct. This construct underwent gelation with changing temperature while its shape was maintained for 7 days. The cells showed over 90% viability and proliferation. By analysing the gene expression pattern in the cells of the construct, the skin regenerative mechanism of the bio-ink was verified. Microarray results confirmed that the gene expression related to skin morphology and development had been enhanced because the bioreactive molecules and growth factors, in addition to residual ECM in dECM, provided an optimal condition for the HDFs. |
| format | Article |
| id | doaj-art-6f45179ddcc04261a4c037ea0b0bd2fb |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-6f45179ddcc04261a4c037ea0b0bd2fb2025-08-20T03:51:24ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-0147164464910.1080/21691401.2019.1575842A potential dermal substitute using decellularized dermis extracellular matrix derived bio-inkJoo-Yun Won0Mi-Hee Lee1Mi-Jeong Kim2Kyung-Hyun Min3Geunseon Ahn4Ji-Seok Han5Songwan Jin6Won-Soo Yun7Jin-Hyung Shim8Research Institute, T&R Biofab Co. Ltd., Siheung-si, Republic of KoreaResearch Institute, T&R Biofab Co. Ltd., Siheung-si, Republic of KoreaResearch Institute, T&R Biofab Co. Ltd., Siheung-si, Republic of KoreaResearch Institute, T&R Biofab Co. Ltd., Siheung-si, Republic of KoreaResearch Institute, T&R Biofab Co. Ltd., Siheung-si, Republic of KoreaDepartment of Advanced Toxicology Research, Korea Institute of Toxicology (KIT), Daejeon, Republic of KoreaDepartment of Mechanical Engineering, Korea Polytechnic University, Siheung-si, Republic of KoreaDepartment of Mechanical Engineering, Korea Polytechnic University, Siheung-si, Republic of KoreaDepartment of Mechanical Engineering, Korea Polytechnic University, Siheung-si, Republic of KoreaUpon bioprinting, cells are mixed with a biomaterial to fabricate a living tissue, thus emphasizing the importance of biomaterials. The biomaterial used in this study was a bio-ink prepared using skin decellularized extracellular matrix (dECM). Skin dECM was extracted by treating the dermis with chemicals and enzymes; the basic structural and functional proteins of the ECM, including collagen, glycosaminoglycans (GAGs), bioreactive materials and growth factors, were preserved, whereas the resident cells that might cause immune rejection or inflammatory responses were removed. The bio-ink based on dECM powder, together with human dermal fibroblasts (HDFs), was loaded into the nozzle of the 3D bioprinter to create the 3D construct. This construct underwent gelation with changing temperature while its shape was maintained for 7 days. The cells showed over 90% viability and proliferation. By analysing the gene expression pattern in the cells of the construct, the skin regenerative mechanism of the bio-ink was verified. Microarray results confirmed that the gene expression related to skin morphology and development had been enhanced because the bioreactive molecules and growth factors, in addition to residual ECM in dECM, provided an optimal condition for the HDFs.https://www.tandfonline.com/doi/10.1080/21691401.2019.1575842dECMbio-ink3D bioprintingmicroarraytissue regeneration |
| spellingShingle | Joo-Yun Won Mi-Hee Lee Mi-Jeong Kim Kyung-Hyun Min Geunseon Ahn Ji-Seok Han Songwan Jin Won-Soo Yun Jin-Hyung Shim A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink Artificial Cells, Nanomedicine, and Biotechnology dECM bio-ink 3D bioprinting microarray tissue regeneration |
| title | A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink |
| title_full | A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink |
| title_fullStr | A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink |
| title_full_unstemmed | A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink |
| title_short | A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink |
| title_sort | potential dermal substitute using decellularized dermis extracellular matrix derived bio ink |
| topic | dECM bio-ink 3D bioprinting microarray tissue regeneration |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1575842 |
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