Bacterial Consortium Biofilm-Based Electrochemical Biosensor for Measurement of Antioxidant Polyphenolic Compounds
This work describes the development of an electrochemical biosensor method based on bacterial consortia to determine antioxidant capacity. The bacterial consortium used is a combination of bacteria from the genera <i>Bacillus</i> and <i>Pseudomonas</i> which can produce the e...
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MDPI AG
2024-11-01
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| Series: | Electrochem |
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| Online Access: | https://www.mdpi.com/2673-3293/5/4/34 |
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| author | Rani Melati Sukma Dyah Iswantini Novik Nurhidayat Mohamad Rafi |
| author_facet | Rani Melati Sukma Dyah Iswantini Novik Nurhidayat Mohamad Rafi |
| author_sort | Rani Melati Sukma |
| collection | DOAJ |
| description | This work describes the development of an electrochemical biosensor method based on bacterial consortia to determine antioxidant capacity. The bacterial consortium used is a combination of bacteria from the genera <i>Bacillus</i> and <i>Pseudomonas</i> which can produce the enzymes tyrosinase and laccase. The consortium bacteria were immobilized on the surface of the screen-printed carbon electrode (SPCE) to form a biofilm. Biofilms were selected based on the highest current response evaluated electrochemically using cyclic voltammetry analysis techniques. Optimum consortium biofilm conditions were obtained in a phosphate buffer solution of pH 7, and biofilm formation occurred on day 7. This work produces analytical performance with a coefficient of determination (R<sup>2</sup>) of 0.9924. The limit of detection (LOD) and limit of quantification (LOQ) values are 0.5 µM and 10 µM, respectively. The biosensor showed a stable response until the 10th week. This biosensor was used to measure the antioxidant capacity of five extracts, and the results were confirmed using a standard method, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The highest antioxidant capacity is guava extract and the lowest is tempuyung extract. Thus, the development of this biosensor method can be used as an alternative for measuring antioxidant capacity. |
| format | Article |
| id | doaj-art-6f3c44e282a2448bb5cf00d5cc43d24e |
| institution | Kabale University |
| issn | 2673-3293 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Electrochem |
| spelling | doaj-art-6f3c44e282a2448bb5cf00d5cc43d24e2024-12-27T14:22:55ZengMDPI AGElectrochem2673-32932024-11-015453054510.3390/electrochem5040034Bacterial Consortium Biofilm-Based Electrochemical Biosensor for Measurement of Antioxidant Polyphenolic CompoundsRani Melati Sukma0Dyah Iswantini1Novik Nurhidayat2Mohamad Rafi3Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, IndonesiaResearch Centre for Applied Microbiology, National Research and Innovation Agency, Cibinong 16911, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, IndonesiaThis work describes the development of an electrochemical biosensor method based on bacterial consortia to determine antioxidant capacity. The bacterial consortium used is a combination of bacteria from the genera <i>Bacillus</i> and <i>Pseudomonas</i> which can produce the enzymes tyrosinase and laccase. The consortium bacteria were immobilized on the surface of the screen-printed carbon electrode (SPCE) to form a biofilm. Biofilms were selected based on the highest current response evaluated electrochemically using cyclic voltammetry analysis techniques. Optimum consortium biofilm conditions were obtained in a phosphate buffer solution of pH 7, and biofilm formation occurred on day 7. This work produces analytical performance with a coefficient of determination (R<sup>2</sup>) of 0.9924. The limit of detection (LOD) and limit of quantification (LOQ) values are 0.5 µM and 10 µM, respectively. The biosensor showed a stable response until the 10th week. This biosensor was used to measure the antioxidant capacity of five extracts, and the results were confirmed using a standard method, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The highest antioxidant capacity is guava extract and the lowest is tempuyung extract. Thus, the development of this biosensor method can be used as an alternative for measuring antioxidant capacity.https://www.mdpi.com/2673-3293/5/4/34antioxidantsbiosensorbiofilm consortiumelectrochemical methodpolyphenol |
| spellingShingle | Rani Melati Sukma Dyah Iswantini Novik Nurhidayat Mohamad Rafi Bacterial Consortium Biofilm-Based Electrochemical Biosensor for Measurement of Antioxidant Polyphenolic Compounds Electrochem antioxidants biosensor biofilm consortium electrochemical method polyphenol |
| title | Bacterial Consortium Biofilm-Based Electrochemical Biosensor for Measurement of Antioxidant Polyphenolic Compounds |
| title_full | Bacterial Consortium Biofilm-Based Electrochemical Biosensor for Measurement of Antioxidant Polyphenolic Compounds |
| title_fullStr | Bacterial Consortium Biofilm-Based Electrochemical Biosensor for Measurement of Antioxidant Polyphenolic Compounds |
| title_full_unstemmed | Bacterial Consortium Biofilm-Based Electrochemical Biosensor for Measurement of Antioxidant Polyphenolic Compounds |
| title_short | Bacterial Consortium Biofilm-Based Electrochemical Biosensor for Measurement of Antioxidant Polyphenolic Compounds |
| title_sort | bacterial consortium biofilm based electrochemical biosensor for measurement of antioxidant polyphenolic compounds |
| topic | antioxidants biosensor biofilm consortium electrochemical method polyphenol |
| url | https://www.mdpi.com/2673-3293/5/4/34 |
| work_keys_str_mv | AT ranimelatisukma bacterialconsortiumbiofilmbasedelectrochemicalbiosensorformeasurementofantioxidantpolyphenoliccompounds AT dyahiswantini bacterialconsortiumbiofilmbasedelectrochemicalbiosensorformeasurementofantioxidantpolyphenoliccompounds AT noviknurhidayat bacterialconsortiumbiofilmbasedelectrochemicalbiosensorformeasurementofantioxidantpolyphenoliccompounds AT mohamadrafi bacterialconsortiumbiofilmbasedelectrochemicalbiosensorformeasurementofantioxidantpolyphenoliccompounds |