Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review
ABSTRACT Sexually transmitted infections (STIs) remain a significant public health concern. Given the asymptomatic nature of many STIs, diagnostic testing is critical for determining the appropriate treatment, enabling effective tracing and reducing the risk of further transmission. Nucleic acid amp...
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | Microbial Biotechnology |
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| Online Access: | https://doi.org/10.1111/1751-7915.70153 |
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| author | Yasaman Ahmadi Yejiong Yu Zhanfeng Cui Wei E. Huang Monique I. Andersson |
| author_facet | Yasaman Ahmadi Yejiong Yu Zhanfeng Cui Wei E. Huang Monique I. Andersson |
| author_sort | Yasaman Ahmadi |
| collection | DOAJ |
| description | ABSTRACT Sexually transmitted infections (STIs) remain a significant public health concern. Given the asymptomatic nature of many STIs, diagnostic testing is critical for determining the appropriate treatment, enabling effective tracing and reducing the risk of further transmission. Nucleic acid amplification tests (NAATs) are the most sensitive and the most widely used in well‐resourced settings. The majority of available NAATs are based on polymerase chain reaction (PCR), which requires highly trained personnel and costly equipment, making it impractical for resource‐limited settings. Loop‐mediated isothermal amplification (LAMP) has emerged as a simple, rapid, sensitive and low‐cost alternative for pathogen detection, particularly well‐suited for point‐of‐care tests (POCT). In this review, we evaluate LAMP assays reported in the literature for the detection of pathogens linked to the high incidence STIs prioritised by the World Health Organization (WHO) for POCT in 2023. These include Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, T. pallidum subspecies pallidum, as well as other common STIs such as herpes simplex virus, hepatitis B virus and human immunodeficiency virus (HIV). For each LAMP assay, we identified and summarised the key elements such as the type and number of tested clinical specimens, chosen target gene, detection system, reference test and clinical outcomes. We highlight the advantages and limitations of these assays and discuss the gaps that should be addressed to improve their applicability for POCT. |
| format | Article |
| id | doaj-art-6f3b5392d6fe47ba89f94d3aedcdda6f |
| institution | DOAJ |
| issn | 1751-7915 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
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| series | Microbial Biotechnology |
| spelling | doaj-art-6f3b5392d6fe47ba89f94d3aedcdda6f2025-08-20T03:12:35ZengWileyMicrobial Biotechnology1751-79152025-05-01185n/an/a10.1111/1751-7915.70153Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A ReviewYasaman Ahmadi0Yejiong Yu1Zhanfeng Cui2Wei E. Huang3Monique I. Andersson4Department of Engineering Science University of Oxford Oxford UKDepartment of Engineering Science University of Oxford Oxford UKDepartment of Engineering Science University of Oxford Oxford UKDepartment of Engineering Science University of Oxford Oxford UKDepartment of Microbiology Oxford University Hospitals NHS Foundation Trust Oxford UKABSTRACT Sexually transmitted infections (STIs) remain a significant public health concern. Given the asymptomatic nature of many STIs, diagnostic testing is critical for determining the appropriate treatment, enabling effective tracing and reducing the risk of further transmission. Nucleic acid amplification tests (NAATs) are the most sensitive and the most widely used in well‐resourced settings. The majority of available NAATs are based on polymerase chain reaction (PCR), which requires highly trained personnel and costly equipment, making it impractical for resource‐limited settings. Loop‐mediated isothermal amplification (LAMP) has emerged as a simple, rapid, sensitive and low‐cost alternative for pathogen detection, particularly well‐suited for point‐of‐care tests (POCT). In this review, we evaluate LAMP assays reported in the literature for the detection of pathogens linked to the high incidence STIs prioritised by the World Health Organization (WHO) for POCT in 2023. These include Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, T. pallidum subspecies pallidum, as well as other common STIs such as herpes simplex virus, hepatitis B virus and human immunodeficiency virus (HIV). For each LAMP assay, we identified and summarised the key elements such as the type and number of tested clinical specimens, chosen target gene, detection system, reference test and clinical outcomes. We highlight the advantages and limitations of these assays and discuss the gaps that should be addressed to improve their applicability for POCT.https://doi.org/10.1111/1751-7915.70153Chlamydia trachomatishepatitis B virusherpes simplex virushuman immunodeficiency virus (HIV)loop‐mediated isothermal amplification (LAMP)Neisseria gonorrhoeae |
| spellingShingle | Yasaman Ahmadi Yejiong Yu Zhanfeng Cui Wei E. Huang Monique I. Andersson Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review Microbial Biotechnology Chlamydia trachomatis hepatitis B virus herpes simplex virus human immunodeficiency virus (HIV) loop‐mediated isothermal amplification (LAMP) Neisseria gonorrhoeae |
| title | Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review |
| title_full | Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review |
| title_fullStr | Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review |
| title_full_unstemmed | Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review |
| title_short | Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review |
| title_sort | loop mediated isothermal amplification lamp for the diagnosis of sexually transmitted infections a review |
| topic | Chlamydia trachomatis hepatitis B virus herpes simplex virus human immunodeficiency virus (HIV) loop‐mediated isothermal amplification (LAMP) Neisseria gonorrhoeae |
| url | https://doi.org/10.1111/1751-7915.70153 |
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