The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trial

Abstract Impaired gut barrier function may lead to progression of liver fibrosis in people with alcohol-related liver disease. The postbiotic ReFerm® can lower gut barrier permeability and may thereby  reduce fibrosis formation. Here, we report the results from an open-labelled, single centre random...

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Main Authors: Johanne K. Hansen, Mads Israelsen, Suguru Nishijima, Sara E. Stinson, Peter Andersen, Stine Johansen, Camilla D. Hansen, Maximilian Joseph Brol, Sabine Klein, Robert Schierwagen, Frank Erhard Uschner, Karolina Sulek, Ida F. Villesen, Katrine P. Lindvig, Katrine H. Thorhauge, Nikolaj Torp, Jane M. Jensen, Marisa Isabell Keller, Gitte H. Jensen, Sönke Detlefsen, Diana J. Leeming, Evelina Stankevic, Tommi Suvitaival, Andressa Zawadzki, Michael Kuhn, Lars Juhl Jensen, Morten Karsdal, Jonel Trebicka, Hans Israelsen, Cristina Legido-Quigley, Peer Bork, Manimozhiyan Arumugam, Torben Hansen, Maja Thiele, Aleksander Krag
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-60755-9
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author Johanne K. Hansen
Mads Israelsen
Suguru Nishijima
Sara E. Stinson
Peter Andersen
Stine Johansen
Camilla D. Hansen
Maximilian Joseph Brol
Sabine Klein
Robert Schierwagen
Frank Erhard Uschner
Karolina Sulek
Ida F. Villesen
Katrine P. Lindvig
Katrine H. Thorhauge
Nikolaj Torp
Jane M. Jensen
Marisa Isabell Keller
Gitte H. Jensen
Sönke Detlefsen
Diana J. Leeming
Evelina Stankevic
Tommi Suvitaival
Andressa Zawadzki
Michael Kuhn
Lars Juhl Jensen
Morten Karsdal
Jonel Trebicka
Hans Israelsen
Cristina Legido-Quigley
Peer Bork
Manimozhiyan Arumugam
Torben Hansen
Maja Thiele
Aleksander Krag
author_facet Johanne K. Hansen
Mads Israelsen
Suguru Nishijima
Sara E. Stinson
Peter Andersen
Stine Johansen
Camilla D. Hansen
Maximilian Joseph Brol
Sabine Klein
Robert Schierwagen
Frank Erhard Uschner
Karolina Sulek
Ida F. Villesen
Katrine P. Lindvig
Katrine H. Thorhauge
Nikolaj Torp
Jane M. Jensen
Marisa Isabell Keller
Gitte H. Jensen
Sönke Detlefsen
Diana J. Leeming
Evelina Stankevic
Tommi Suvitaival
Andressa Zawadzki
Michael Kuhn
Lars Juhl Jensen
Morten Karsdal
Jonel Trebicka
Hans Israelsen
Cristina Legido-Quigley
Peer Bork
Manimozhiyan Arumugam
Torben Hansen
Maja Thiele
Aleksander Krag
author_sort Johanne K. Hansen
collection DOAJ
description Abstract Impaired gut barrier function may lead to progression of liver fibrosis in people with alcohol-related liver disease. The postbiotic ReFerm® can lower gut barrier permeability and may thereby  reduce fibrosis formation. Here, we report the results from an open-labelled, single centre randomized controlled trial where 56 patients with advanced, compensated, alcohol-related liver disease were assigned 1:1 to receive either ReFerm® (n = 28) or standard nutritional support (Fresubin®, n = 28) for 24 weeks. The primary outcome was a ≥ 10% reduction of the fibrosis formation marker alpha-smooth muscle actin in liver biopsies, assessed by a blinded pathologist using automated digital imaging analysis. Paired liver biopsies meeting quality criteria for the primary outcome were available for 40 participants (ReFerm®, n = 21 and Fresubin®, n = 19). This reduction was observed in 29% of patients receiving ReFerm®, compared to 14% with Fresubin® (OR = 2.40; 95% CI 0.63 to 9.16; p = 0.200). No treatment-related serious adverse events occurred. Our findings suggest that ReFerm® may reduce liver fibrosis by enhancing gut barrier function, potentially preventing the progression of alcohol-related liver disease.
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spelling doaj-art-6f3a0d8a37b948aea8361bda69da64602025-08-20T04:01:41ZengNature PortfolioNature Communications2041-17232025-07-0116111610.1038/s41467-025-60755-9The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trialJohanne K. Hansen0Mads Israelsen1Suguru Nishijima2Sara E. Stinson3Peter Andersen4Stine Johansen5Camilla D. Hansen6Maximilian Joseph Brol7Sabine Klein8Robert Schierwagen9Frank Erhard Uschner10Karolina Sulek11Ida F. Villesen12Katrine P. Lindvig13Katrine H. Thorhauge14Nikolaj Torp15Jane M. Jensen16Marisa Isabell Keller17Gitte H. Jensen18Sönke Detlefsen19Diana J. Leeming20Evelina Stankevic21Tommi Suvitaival22Andressa Zawadzki23Michael Kuhn24Lars Juhl Jensen25Morten Karsdal26Jonel Trebicka27Hans Israelsen28Cristina Legido-Quigley29Peer Bork30Manimozhiyan Arumugam31Torben Hansen32Maja Thiele33Aleksander Krag34Department of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalMolecular Systems Biology Unit, European Molecular Biology LaboratoryNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Internal Medicine B, University of MünsterDepartment of Internal Medicine B, University of MünsterDepartment of Internal Medicine B, University of MünsterDepartment of Internal Medicine B, University of MünsterSteno Diabetes Center CopenhagenDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalMolecular Systems Biology Unit, European Molecular Biology LaboratoryDepartment of Pathology, Odense University HospitalDepartment of Clinical Research, Faculty of Health Sciences, University of Southern DenmarkNordic Bioscience A/S, Biomarkers & ResearchNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of CopenhagenSteno Diabetes Center CopenhagenSteno Diabetes Center CopenhagenMolecular Systems Biology Unit, European Molecular Biology LaboratoryNovo Nordisk Foundation Center for Protein Research, CPR, University of CopenhagenNordic Bioscience A/S, Biomarkers & ResearchDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalNordic Rebalance A/SSteno Diabetes Center CopenhagenMolecular Systems Biology Unit, European Molecular Biology LaboratoryDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalDepartment of Gastroenterology and Hepatology, Centre for Liver Research, Odense University HospitalAbstract Impaired gut barrier function may lead to progression of liver fibrosis in people with alcohol-related liver disease. The postbiotic ReFerm® can lower gut barrier permeability and may thereby  reduce fibrosis formation. Here, we report the results from an open-labelled, single centre randomized controlled trial where 56 patients with advanced, compensated, alcohol-related liver disease were assigned 1:1 to receive either ReFerm® (n = 28) or standard nutritional support (Fresubin®, n = 28) for 24 weeks. The primary outcome was a ≥ 10% reduction of the fibrosis formation marker alpha-smooth muscle actin in liver biopsies, assessed by a blinded pathologist using automated digital imaging analysis. Paired liver biopsies meeting quality criteria for the primary outcome were available for 40 participants (ReFerm®, n = 21 and Fresubin®, n = 19). This reduction was observed in 29% of patients receiving ReFerm®, compared to 14% with Fresubin® (OR = 2.40; 95% CI 0.63 to 9.16; p = 0.200). No treatment-related serious adverse events occurred. Our findings suggest that ReFerm® may reduce liver fibrosis by enhancing gut barrier function, potentially preventing the progression of alcohol-related liver disease.https://doi.org/10.1038/s41467-025-60755-9
spellingShingle Johanne K. Hansen
Mads Israelsen
Suguru Nishijima
Sara E. Stinson
Peter Andersen
Stine Johansen
Camilla D. Hansen
Maximilian Joseph Brol
Sabine Klein
Robert Schierwagen
Frank Erhard Uschner
Karolina Sulek
Ida F. Villesen
Katrine P. Lindvig
Katrine H. Thorhauge
Nikolaj Torp
Jane M. Jensen
Marisa Isabell Keller
Gitte H. Jensen
Sönke Detlefsen
Diana J. Leeming
Evelina Stankevic
Tommi Suvitaival
Andressa Zawadzki
Michael Kuhn
Lars Juhl Jensen
Morten Karsdal
Jonel Trebicka
Hans Israelsen
Cristina Legido-Quigley
Peer Bork
Manimozhiyan Arumugam
Torben Hansen
Maja Thiele
Aleksander Krag
The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trial
Nature Communications
title The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trial
title_full The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trial
title_fullStr The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trial
title_full_unstemmed The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trial
title_short The postbiotic ReFerm® versus standard nutritional support in advanced alcohol-related liver disease (GALA-POSTBIO): a randomized controlled phase 2 trial
title_sort postbiotic referm r versus standard nutritional support in advanced alcohol related liver disease gala postbio a randomized controlled phase 2 trial
url https://doi.org/10.1038/s41467-025-60755-9
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