hUC-MSC Combined with DHEA Alleviates Ovarian Senescence in Naturally Aging Mice through Enhancing Antioxidant Capacity and Inhibiting Inflammatory Response
The ovary is an important organ for women to maintain reproductive and endocrine functions. Ovarian aging can lead to female reproductive aging, which is a key factor causing rapid aging of the female body. Umbilical cord-derived MSCs (UC-MSCs) play a therapeutic role in various degenerative disease...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2024/3100942 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850222320528916480 |
|---|---|
| author | Chun-Yi Guan Dan Zhang Xue-Cheng Sun Xu Ma Hong-Fei Xia |
| author_facet | Chun-Yi Guan Dan Zhang Xue-Cheng Sun Xu Ma Hong-Fei Xia |
| author_sort | Chun-Yi Guan |
| collection | DOAJ |
| description | The ovary is an important organ for women to maintain reproductive and endocrine functions. Ovarian aging can lead to female reproductive aging, which is a key factor causing rapid aging of the female body. Umbilical cord-derived MSCs (UC-MSCs) play a therapeutic role in various degenerative diseases. Dehydroepiandrosterone (DHEA) is widely used in the treatment of reversing oocyte quality. However, it is unclear whether UC-MSCs combined with DHEA supplementation can improve ovarian senescence in naturally aging mice. To address this question, we studied the influence of the combination of human UC-MSCs (hUC-MSCs) and DHEA on ovarian morphology and function in naturally aging mice. The results showed a significant augmentation in the number of primary follicles, as well as a significant upregulation of estradiol (E2), follicle-stimulating hormone (FSH), and anti-Mullerian hormone (AMH) hormone levels, and a significant increase in survival rate in naturally aging mice treated by hUC-MSCs and DHEA. Moreover, the combination of hUC-MSCs and DHEA significantly reduced the reactive oxygen species (ROS) level and downregulated the expression levels of proinflammatory factors IL-6, IL-18, and TNF-α. Furthermore, the PI3K/AKT/mTOR pathway was inhibited. Conclusively, the combination therapy of hUC-MSC + DHEA contributed to restore ovarian function in aging mice and extend their lifespan by restoring hormone levels and inhibiting inflammatory factors. |
| format | Article |
| id | doaj-art-6f2e5d70b892465dbb3987d9c34d5a59 |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6f2e5d70b892465dbb3987d9c34d5a592025-08-20T02:06:23ZengWileyStem Cells International1687-96782024-01-01202410.1155/2024/3100942hUC-MSC Combined with DHEA Alleviates Ovarian Senescence in Naturally Aging Mice through Enhancing Antioxidant Capacity and Inhibiting Inflammatory ResponseChun-Yi Guan0Dan Zhang1Xue-Cheng Sun2Xu Ma3Hong-Fei Xia4Reproductive and Genetic Center and NHC Key Laboratory of Reproductive Health Engineering Technology ResearchGraduate SchoolGraduate SchoolReproductive and Genetic Center and NHC Key Laboratory of Reproductive Health Engineering Technology ResearchReproductive and Genetic Center and NHC Key Laboratory of Reproductive Health Engineering Technology ResearchThe ovary is an important organ for women to maintain reproductive and endocrine functions. Ovarian aging can lead to female reproductive aging, which is a key factor causing rapid aging of the female body. Umbilical cord-derived MSCs (UC-MSCs) play a therapeutic role in various degenerative diseases. Dehydroepiandrosterone (DHEA) is widely used in the treatment of reversing oocyte quality. However, it is unclear whether UC-MSCs combined with DHEA supplementation can improve ovarian senescence in naturally aging mice. To address this question, we studied the influence of the combination of human UC-MSCs (hUC-MSCs) and DHEA on ovarian morphology and function in naturally aging mice. The results showed a significant augmentation in the number of primary follicles, as well as a significant upregulation of estradiol (E2), follicle-stimulating hormone (FSH), and anti-Mullerian hormone (AMH) hormone levels, and a significant increase in survival rate in naturally aging mice treated by hUC-MSCs and DHEA. Moreover, the combination of hUC-MSCs and DHEA significantly reduced the reactive oxygen species (ROS) level and downregulated the expression levels of proinflammatory factors IL-6, IL-18, and TNF-α. Furthermore, the PI3K/AKT/mTOR pathway was inhibited. Conclusively, the combination therapy of hUC-MSC + DHEA contributed to restore ovarian function in aging mice and extend their lifespan by restoring hormone levels and inhibiting inflammatory factors.http://dx.doi.org/10.1155/2024/3100942 |
| spellingShingle | Chun-Yi Guan Dan Zhang Xue-Cheng Sun Xu Ma Hong-Fei Xia hUC-MSC Combined with DHEA Alleviates Ovarian Senescence in Naturally Aging Mice through Enhancing Antioxidant Capacity and Inhibiting Inflammatory Response Stem Cells International |
| title | hUC-MSC Combined with DHEA Alleviates Ovarian Senescence in Naturally Aging Mice through Enhancing Antioxidant Capacity and Inhibiting Inflammatory Response |
| title_full | hUC-MSC Combined with DHEA Alleviates Ovarian Senescence in Naturally Aging Mice through Enhancing Antioxidant Capacity and Inhibiting Inflammatory Response |
| title_fullStr | hUC-MSC Combined with DHEA Alleviates Ovarian Senescence in Naturally Aging Mice through Enhancing Antioxidant Capacity and Inhibiting Inflammatory Response |
| title_full_unstemmed | hUC-MSC Combined with DHEA Alleviates Ovarian Senescence in Naturally Aging Mice through Enhancing Antioxidant Capacity and Inhibiting Inflammatory Response |
| title_short | hUC-MSC Combined with DHEA Alleviates Ovarian Senescence in Naturally Aging Mice through Enhancing Antioxidant Capacity and Inhibiting Inflammatory Response |
| title_sort | huc msc combined with dhea alleviates ovarian senescence in naturally aging mice through enhancing antioxidant capacity and inhibiting inflammatory response |
| url | http://dx.doi.org/10.1155/2024/3100942 |
| work_keys_str_mv | AT chunyiguan hucmsccombinedwithdheaalleviatesovariansenescenceinnaturallyagingmicethroughenhancingantioxidantcapacityandinhibitinginflammatoryresponse AT danzhang hucmsccombinedwithdheaalleviatesovariansenescenceinnaturallyagingmicethroughenhancingantioxidantcapacityandinhibitinginflammatoryresponse AT xuechengsun hucmsccombinedwithdheaalleviatesovariansenescenceinnaturallyagingmicethroughenhancingantioxidantcapacityandinhibitinginflammatoryresponse AT xuma hucmsccombinedwithdheaalleviatesovariansenescenceinnaturallyagingmicethroughenhancingantioxidantcapacityandinhibitinginflammatoryresponse AT hongfeixia hucmsccombinedwithdheaalleviatesovariansenescenceinnaturallyagingmicethroughenhancingantioxidantcapacityandinhibitinginflammatoryresponse |