Growth and genetic stimulation of Cattleya-91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditions
ABSTRACT Effective Cattleya micropropagation enables achieving enhanced seedling quality and production. Ultrafine bubbles (UFBs) represent a recent technological breakthrough that is expected to improve the growth and development of orchid plantlets. The objective of this research was to reveal the...
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Universidade Federal de Lavras
2025-06-01
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| Series: | Ciência e Agrotecnologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542025000100221&lng=en&tlng=en |
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| author | Dita Agisimanto Farida Yulianti Mitalarasati Abdul Karim Fanani Hardiyanto Nirmala Friyanti Devy Anto Tri Sugiarto Budi Winarto |
| author_facet | Dita Agisimanto Farida Yulianti Mitalarasati Abdul Karim Fanani Hardiyanto Nirmala Friyanti Devy Anto Tri Sugiarto Budi Winarto |
| author_sort | Dita Agisimanto |
| collection | DOAJ |
| description | ABSTRACT Effective Cattleya micropropagation enables achieving enhanced seedling quality and production. Ultrafine bubbles (UFBs) represent a recent technological breakthrough that is expected to improve the growth and development of orchid plantlets. The objective of this research was to reveal the effects of oxygenated UFB (O-UFB) at different liquid nutrition strengths on the morpho-physiology and gene expression of Cattleya plantlets grown in vitro under septic conditions. Uniform plantlets were planted on granular vermiculite-perlite (VP) (1:1) with full-Murashige Skoog (MS), –-MS, ½-MS, and full-Hougland (HG) liquid media enriched with oxygenated UFB (O-UFB), and full-MS-0 was used as control. A lower ionic strength (–-MS) medium with the presence of O-UFB improved Cattleya plantlet growth both morphologically and physiologically. Nutrition strength also induced the overexpression of SUT2 and PEPCK genes during the in vitro septic culture, leading to a physiological transition from heterotrophic to autotrophic micropropagation. Therefore, O-UFB supplementation during Cattleya plantlet growth and development can improve its quality and productivity. |
| format | Article |
| id | doaj-art-6f1c243b4d0b4091a8ecc917c5f70252 |
| institution | DOAJ |
| issn | 1981-1829 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Universidade Federal de Lavras |
| record_format | Article |
| series | Ciência e Agrotecnologia |
| spelling | doaj-art-6f1c243b4d0b4091a8ecc917c5f702522025-08-20T03:23:52ZengUniversidade Federal de LavrasCiência e Agrotecnologia1981-18292025-06-014910.1590/1413-7054202549026224Growth and genetic stimulation of Cattleya-91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditionsDita Agisimantohttps://orcid.org/0000-0003-3265-2292Farida Yuliantihttps://orcid.org/0000-0003-1911-2611 Mitalarasatihttps://orcid.org/0009-0004-2789-5067Abdul Karim Fananihttps://orcid.org/0009-0000-1862-9446 Hardiyantohttps://orcid.org/0000-0001-9146-4094Nirmala Friyanti Devyhttps://orcid.org/0000-0003-2478-5255Anto Tri Sugiartohttps://orcid.org/0000-0002-6809-9882Budi Winartohttps://orcid.org/0009-0000-6603-1959ABSTRACT Effective Cattleya micropropagation enables achieving enhanced seedling quality and production. Ultrafine bubbles (UFBs) represent a recent technological breakthrough that is expected to improve the growth and development of orchid plantlets. The objective of this research was to reveal the effects of oxygenated UFB (O-UFB) at different liquid nutrition strengths on the morpho-physiology and gene expression of Cattleya plantlets grown in vitro under septic conditions. Uniform plantlets were planted on granular vermiculite-perlite (VP) (1:1) with full-Murashige Skoog (MS), –-MS, ½-MS, and full-Hougland (HG) liquid media enriched with oxygenated UFB (O-UFB), and full-MS-0 was used as control. A lower ionic strength (–-MS) medium with the presence of O-UFB improved Cattleya plantlet growth both morphologically and physiologically. Nutrition strength also induced the overexpression of SUT2 and PEPCK genes during the in vitro septic culture, leading to a physiological transition from heterotrophic to autotrophic micropropagation. Therefore, O-UFB supplementation during Cattleya plantlet growth and development can improve its quality and productivity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542025000100221&lng=en&tlng=enGene expressionmicropropagationnanobubblesorchidionic strength |
| spellingShingle | Dita Agisimanto Farida Yulianti Mitalarasati Abdul Karim Fanani Hardiyanto Nirmala Friyanti Devy Anto Tri Sugiarto Budi Winarto Growth and genetic stimulation of Cattleya-91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditions Ciência e Agrotecnologia Gene expression micropropagation nanobubbles orchid ionic strength |
| title | Growth and genetic stimulation of Cattleya-91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditions |
| title_full | Growth and genetic stimulation of Cattleya-91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditions |
| title_fullStr | Growth and genetic stimulation of Cattleya-91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditions |
| title_full_unstemmed | Growth and genetic stimulation of Cattleya-91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditions |
| title_short | Growth and genetic stimulation of Cattleya-91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditions |
| title_sort | growth and genetic stimulation of cattleya 91 plantlets enriched with oxygenated ultrafine bubbles in vitro under aseptic conditions |
| topic | Gene expression micropropagation nanobubbles orchid ionic strength |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542025000100221&lng=en&tlng=en |
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