Safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non‐genetically modified Mucor circinelloides strain AE‐LMH

Abstract The food enzyme triacylglycerol lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) is produced with the non‐genetically modified Mucor circinelloides strain AE‐LMH by Amano Enzyme Inc. A safety evaluation of this food enzyme was made previously, in which EFSA concluded that this food enzyme...

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Main Authors: EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Claude Lambré, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Yrjö Roos, Yi Liu, Eleonora Marini, Giulio diPiazza, Andrew Chesson
Format: Article
Language:English
Published: Wiley 2024-04-01
Series:EFSA Journal
Subjects:
Online Access:https://doi.org/10.2903/j.efsa.2024.8699
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author EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP)
Claude Lambré
José Manuel Barat Baviera
Claudia Bolognesi
Pier Sandro Cocconcelli
Riccardo Crebelli
David Michael Gott
Konrad Grob
Evgenia Lampi
Marcel Mengelers
Alicja Mortensen
Gilles Rivière
Inger‐Lise Steffensen
Christina Tlustos
Henk Van Loveren
Laurence Vernis
Holger Zorn
Yrjö Roos
Yi Liu
Eleonora Marini
Giulio diPiazza
Andrew Chesson
author_facet EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP)
Claude Lambré
José Manuel Barat Baviera
Claudia Bolognesi
Pier Sandro Cocconcelli
Riccardo Crebelli
David Michael Gott
Konrad Grob
Evgenia Lampi
Marcel Mengelers
Alicja Mortensen
Gilles Rivière
Inger‐Lise Steffensen
Christina Tlustos
Henk Van Loveren
Laurence Vernis
Holger Zorn
Yrjö Roos
Yi Liu
Eleonora Marini
Giulio diPiazza
Andrew Chesson
author_sort EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP)
collection DOAJ
description Abstract The food enzyme triacylglycerol lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) is produced with the non‐genetically modified Mucor circinelloides strain AE‐LMH by Amano Enzyme Inc. A safety evaluation of this food enzyme was made previously, in which EFSA concluded that this food enzyme did not give rise to safety concerns when used in three food manufacturing processes. Subsequently, the applicant requested to extend its use to include two additional processes. In this assessment, EFSA updated the safety evaluation of this food enzyme when used in a total of five food manufacturing processes. The dietary exposure to the food enzyme‐total organic solids (TOS) was estimated to be up to 0.845 mg TOS/kg body weight (bw) per day in European populations. When combined with the no observed adverse effect level previously reported (784 mg TOS/kg bw per day, the highest dose tested), the Panel derived a margin of exposure of at least 928. Based on the data provided for the previous evaluation and the revised margin of exposure, the Panel concluded that this food enzyme does not give rise to safety concerns under the revised intended conditions of use.
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spelling doaj-art-6f0d0693d0f245119e963e4e3640b3992025-08-20T02:43:58ZengWileyEFSA Journal1831-47322024-04-01224n/an/a10.2903/j.efsa.2024.8699Safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non‐genetically modified Mucor circinelloides strain AE‐LMHEFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP)Claude LambréJosé Manuel Barat BavieraClaudia BolognesiPier Sandro CocconcelliRiccardo CrebelliDavid Michael GottKonrad GrobEvgenia LampiMarcel MengelersAlicja MortensenGilles RivièreInger‐Lise SteffensenChristina TlustosHenk Van LoverenLaurence VernisHolger ZornYrjö RoosYi LiuEleonora MariniGiulio diPiazzaAndrew ChessonAbstract The food enzyme triacylglycerol lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) is produced with the non‐genetically modified Mucor circinelloides strain AE‐LMH by Amano Enzyme Inc. A safety evaluation of this food enzyme was made previously, in which EFSA concluded that this food enzyme did not give rise to safety concerns when used in three food manufacturing processes. Subsequently, the applicant requested to extend its use to include two additional processes. In this assessment, EFSA updated the safety evaluation of this food enzyme when used in a total of five food manufacturing processes. The dietary exposure to the food enzyme‐total organic solids (TOS) was estimated to be up to 0.845 mg TOS/kg body weight (bw) per day in European populations. When combined with the no observed adverse effect level previously reported (784 mg TOS/kg bw per day, the highest dose tested), the Panel derived a margin of exposure of at least 928. Based on the data provided for the previous evaluation and the revised margin of exposure, the Panel concluded that this food enzyme does not give rise to safety concerns under the revised intended conditions of use.https://doi.org/10.2903/j.efsa.2024.8699EC 3.1.1.3EFSA‐Q‐2015‐00692EFSA‐Q‐2023‐00371food enzymeMucor circinelloidesnon‐genetically modified microorganism
spellingShingle EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP)
Claude Lambré
José Manuel Barat Baviera
Claudia Bolognesi
Pier Sandro Cocconcelli
Riccardo Crebelli
David Michael Gott
Konrad Grob
Evgenia Lampi
Marcel Mengelers
Alicja Mortensen
Gilles Rivière
Inger‐Lise Steffensen
Christina Tlustos
Henk Van Loveren
Laurence Vernis
Holger Zorn
Yrjö Roos
Yi Liu
Eleonora Marini
Giulio diPiazza
Andrew Chesson
Safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non‐genetically modified Mucor circinelloides strain AE‐LMH
EFSA Journal
EC 3.1.1.3
EFSA‐Q‐2015‐00692
EFSA‐Q‐2023‐00371
food enzyme
Mucor circinelloides
non‐genetically modified microorganism
title Safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non‐genetically modified Mucor circinelloides strain AE‐LMH
title_full Safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non‐genetically modified Mucor circinelloides strain AE‐LMH
title_fullStr Safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non‐genetically modified Mucor circinelloides strain AE‐LMH
title_full_unstemmed Safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non‐genetically modified Mucor circinelloides strain AE‐LMH
title_short Safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non‐genetically modified Mucor circinelloides strain AE‐LMH
title_sort safety evaluation of an extension of use of the food enzyme triacylglycerol lipase from the non genetically modified mucor circinelloides strain ae lmh
topic EC 3.1.1.3
EFSA‐Q‐2015‐00692
EFSA‐Q‐2023‐00371
food enzyme
Mucor circinelloides
non‐genetically modified microorganism
url https://doi.org/10.2903/j.efsa.2024.8699
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