Multi-omics analysis reveals that Bacillus spp. enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolism
Abstract Background Symbiotic microbiota in vertebrates play critical roles in establishing and enhancing host resistance to pathogenic infections as well as maintaining host homeostasis. The interactions and mechanisms of commensal microbiota-mediated mucosal immune systems have been extensively st...
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BMC
2025-05-01
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| Online Access: | https://doi.org/10.1186/s40168-025-02124-8 |
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| author | Gaofeng Cheng Weiguang Kong Ruiqi Lin Zhihao Jiang Xinyou Wang Xueying Qin Yong Shi Peng Yang Xiaoyun Chen Lu Xia Zhen Xu |
| author_facet | Gaofeng Cheng Weiguang Kong Ruiqi Lin Zhihao Jiang Xinyou Wang Xueying Qin Yong Shi Peng Yang Xiaoyun Chen Lu Xia Zhen Xu |
| author_sort | Gaofeng Cheng |
| collection | DOAJ |
| description | Abstract Background Symbiotic microbiota in vertebrates play critical roles in establishing and enhancing host resistance to pathogenic infections as well as maintaining host homeostasis. The interactions and mechanisms of commensal microbiota-mediated mucosal immune systems have been extensively studied in mammals and, to a lesser extent, in birds. However, despite several studies emphasizing the role of mucosal microbiota in controlling pathogen infections in teleost fish, limited knowledge exists regarding the core microbiota and the mechanisms by which they contribute to resistance against viral infections. Results Our findings suggest that viral infections shape clinical manifestations of varying severity in infected fish. An increased abundance of Bacillus spp. in the mild phenotype indicates its crucial role in influencing fish immunity during viral infections. To confirm that Bacillus spp. act as a core contributor against viral infection in fish, we isolated a representative strain of Bacillus spp. from largemouth bass (Micropterus salmoides), which was identified as Bacillus velezensis (Bv), and subsequently conducted feeding trials. Our study demonstrated that dietary supplementation with Bv significantly reduced mortality from largemouth bass virus (LMBV) infection in bass by enhancing host immunity and metabolism as well as by regulating the microbial community. Furthermore, multi-omics analysis elucidated the mechanism by which Bacillus spp. confer resistance to viral infections by regulating the production of diglyceride (DG) during lipid metabolism. Conclusions Our study provides the first evidence that Bacillus spp. are a core microbiota for combating viral infections in teleost fish, shedding light on the conserved functions of probiotics as a core microbiota in regulating microbial homeostasis and mucosal immunity across the vertebrate lineage. |
| format | Article |
| id | doaj-art-6f00e2b6a1674cf0b976ac18749d6bf0 |
| institution | DOAJ |
| issn | 2049-2618 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | Microbiome |
| spelling | doaj-art-6f00e2b6a1674cf0b976ac18749d6bf02025-08-20T03:10:30ZengBMCMicrobiome2049-26182025-05-0113111710.1186/s40168-025-02124-8Multi-omics analysis reveals that Bacillus spp. enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolismGaofeng Cheng0Weiguang Kong1Ruiqi Lin2Zhihao Jiang3Xinyou Wang4Xueying Qin5Yong Shi6Peng Yang7Xiaoyun Chen8Lu Xia9Zhen Xu10Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural UniversityState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of SciencesLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean UniversityState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of SciencesState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of SciencesDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural UniversityState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of SciencesAbstract Background Symbiotic microbiota in vertebrates play critical roles in establishing and enhancing host resistance to pathogenic infections as well as maintaining host homeostasis. The interactions and mechanisms of commensal microbiota-mediated mucosal immune systems have been extensively studied in mammals and, to a lesser extent, in birds. However, despite several studies emphasizing the role of mucosal microbiota in controlling pathogen infections in teleost fish, limited knowledge exists regarding the core microbiota and the mechanisms by which they contribute to resistance against viral infections. Results Our findings suggest that viral infections shape clinical manifestations of varying severity in infected fish. An increased abundance of Bacillus spp. in the mild phenotype indicates its crucial role in influencing fish immunity during viral infections. To confirm that Bacillus spp. act as a core contributor against viral infection in fish, we isolated a representative strain of Bacillus spp. from largemouth bass (Micropterus salmoides), which was identified as Bacillus velezensis (Bv), and subsequently conducted feeding trials. Our study demonstrated that dietary supplementation with Bv significantly reduced mortality from largemouth bass virus (LMBV) infection in bass by enhancing host immunity and metabolism as well as by regulating the microbial community. Furthermore, multi-omics analysis elucidated the mechanism by which Bacillus spp. confer resistance to viral infections by regulating the production of diglyceride (DG) during lipid metabolism. Conclusions Our study provides the first evidence that Bacillus spp. are a core microbiota for combating viral infections in teleost fish, shedding light on the conserved functions of probiotics as a core microbiota in regulating microbial homeostasis and mucosal immunity across the vertebrate lineage.https://doi.org/10.1186/s40168-025-02124-8Bacillus velezensisSymbiotic microbiotaMulti-omics analysisViral infectionLargemouth bass |
| spellingShingle | Gaofeng Cheng Weiguang Kong Ruiqi Lin Zhihao Jiang Xinyou Wang Xueying Qin Yong Shi Peng Yang Xiaoyun Chen Lu Xia Zhen Xu Multi-omics analysis reveals that Bacillus spp. enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolism Microbiome Bacillus velezensis Symbiotic microbiota Multi-omics analysis Viral infection Largemouth bass |
| title | Multi-omics analysis reveals that Bacillus spp. enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolism |
| title_full | Multi-omics analysis reveals that Bacillus spp. enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolism |
| title_fullStr | Multi-omics analysis reveals that Bacillus spp. enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolism |
| title_full_unstemmed | Multi-omics analysis reveals that Bacillus spp. enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolism |
| title_short | Multi-omics analysis reveals that Bacillus spp. enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolism |
| title_sort | multi omics analysis reveals that bacillus spp enhance mucosal antiviral immunity in teleost fish by mediating diglyceride production through lipid metabolism |
| topic | Bacillus velezensis Symbiotic microbiota Multi-omics analysis Viral infection Largemouth bass |
| url | https://doi.org/10.1186/s40168-025-02124-8 |
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