The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis
Urease-positive urogenital mycoplasmas are considered to be responsible for the formation of urinary stones. They are usually a part of the normal flora in the human urogenital tract, causing asymptomatic infections. However, many symptomatic infections with these bacteria have been reported. <i&...
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2025-07-01
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| author | Dominika Smolec Małgorzata Aptekorz Łukasz Filipczyk Zygmunt Gofron Jacek Zostawa Robert Smolec Tomasz J. Wąsik Alicja Ekiel |
| author_facet | Dominika Smolec Małgorzata Aptekorz Łukasz Filipczyk Zygmunt Gofron Jacek Zostawa Robert Smolec Tomasz J. Wąsik Alicja Ekiel |
| author_sort | Dominika Smolec |
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| description | Urease-positive urogenital mycoplasmas are considered to be responsible for the formation of urinary stones. They are usually a part of the normal flora in the human urogenital tract, causing asymptomatic infections. However, many symptomatic infections with these bacteria have been reported. <i>M. genitalium</i> is recognized as a cause of male urethritis and other common genitourinary diseases. The role of other urogenital mycoplasmas is still unclear. The aim of this study was to estimate the quantitative prevalence of <i>Ureaplasma</i> spp., <i>M. genitalium</i> and <i>M. hominis</i> in men with urolithiasis using quantitative real-time PCR (qPCR). The study group comprised 100 men with urolithiasis. A total of 60 men were included in the control group. Urogenital mycoplasma DNA in urine samples was detected significantly more often among men with urolithiasis than in healthy subjects—43.0% vs. 26.6%, <i>p</i> = 0.0382, respectively. The majority of positive results (38/43) concerned <i>U. parvum</i> species, the frequency of which was higher in the study group (38.0% (38/100)) than in the control group (23.3% (14/60)), <i>p</i> = 0.0552. The median concentration of <i>U. urealyticum</i> DNA was higher in the study group compared with the control, <i>p</i> = 0.5714. However, further studies are needed to confirm the usefulness of quantitative studies in determining the role of urogenital mycoplasmas in pathology. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-6efe773eaaa04ffb8cd75ddae1b656dc2025-08-20T03:32:32ZengMDPI AGPathogens2076-08172025-07-0114767010.3390/pathogens14070670The Quantitative Detection of Urogenital Mycoplasmas in Men with UrolithiasisDominika Smolec0Małgorzata Aptekorz1Łukasz Filipczyk2Zygmunt Gofron3Jacek Zostawa4Robert Smolec5Tomasz J. Wąsik6Alicja Ekiel7Department of Medical Microbiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, PolandDepartment of Medical Microbiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, PolandDepartment of Histology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, PolandMed Holding Emil Michalowski Specialist Hospital, 40-073 Katowice, PolandDepartment of Urology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-800 Zabrze, PolandDepartment of Thoracic Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-800 Zabrze, PolandDepartment of Medical Microbiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, PolandDepartment of Medical Microbiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, PolandUrease-positive urogenital mycoplasmas are considered to be responsible for the formation of urinary stones. They are usually a part of the normal flora in the human urogenital tract, causing asymptomatic infections. However, many symptomatic infections with these bacteria have been reported. <i>M. genitalium</i> is recognized as a cause of male urethritis and other common genitourinary diseases. The role of other urogenital mycoplasmas is still unclear. The aim of this study was to estimate the quantitative prevalence of <i>Ureaplasma</i> spp., <i>M. genitalium</i> and <i>M. hominis</i> in men with urolithiasis using quantitative real-time PCR (qPCR). The study group comprised 100 men with urolithiasis. A total of 60 men were included in the control group. Urogenital mycoplasma DNA in urine samples was detected significantly more often among men with urolithiasis than in healthy subjects—43.0% vs. 26.6%, <i>p</i> = 0.0382, respectively. The majority of positive results (38/43) concerned <i>U. parvum</i> species, the frequency of which was higher in the study group (38.0% (38/100)) than in the control group (23.3% (14/60)), <i>p</i> = 0.0552. The median concentration of <i>U. urealyticum</i> DNA was higher in the study group compared with the control, <i>p</i> = 0.5714. However, further studies are needed to confirm the usefulness of quantitative studies in determining the role of urogenital mycoplasmas in pathology.https://www.mdpi.com/2076-0817/14/7/670qPCR<i>Urogenital mycoplasmas</i>urolithiasis |
| spellingShingle | Dominika Smolec Małgorzata Aptekorz Łukasz Filipczyk Zygmunt Gofron Jacek Zostawa Robert Smolec Tomasz J. Wąsik Alicja Ekiel The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis Pathogens qPCR <i>Urogenital mycoplasmas</i> urolithiasis |
| title | The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis |
| title_full | The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis |
| title_fullStr | The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis |
| title_full_unstemmed | The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis |
| title_short | The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis |
| title_sort | quantitative detection of urogenital mycoplasmas in men with urolithiasis |
| topic | qPCR <i>Urogenital mycoplasmas</i> urolithiasis |
| url | https://www.mdpi.com/2076-0817/14/7/670 |
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