MATRIX: a platform for cultivation profiling and bioactive discovery in natural product research
The MATRIX protocol presents a novel miniaturized 24-well plate approach for microbial cultivation profiling, enabling the efficient and parallel assessment of microbial metabolite production. This method integrates a media MATRIX within a microbioreactor to facilitate the creation of diverse growth...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Natural Products |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fntpr.2024.1532543/full |
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| author | Zeinab G. Khalil Thulasi Sritharan Angela A. Salim Robert J. Capon |
| author_facet | Zeinab G. Khalil Thulasi Sritharan Angela A. Salim Robert J. Capon |
| author_sort | Zeinab G. Khalil |
| collection | DOAJ |
| description | The MATRIX protocol presents a novel miniaturized 24-well plate approach for microbial cultivation profiling, enabling the efficient and parallel assessment of microbial metabolite production. This method integrates a media MATRIX within a microbioreactor to facilitate the creation of diverse growth conditions for microbial cultivation. The 24-well format supports the simultaneous study of multiple microbial strains and conditions, allowing the discovery of novel bioactive compounds. By employing a variety of media compositions and cultivations, MATRIX enhances the understanding of microbial metabolic pathways and interactions. This protocol provides a robust platform for natural product discovery, microbial ecology studies, and drug development, showcasing its potential to uncover new natural products and other valuable bioactives. The MATRIX approach offers a resource-efficient platform for microbial biodiscovery, contributing significantly to natural product research and drug development. |
| format | Article |
| id | doaj-art-6ef78b2598464550ad398cd01e845689 |
| institution | Kabale University |
| issn | 2813-2602 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Natural Products |
| spelling | doaj-art-6ef78b2598464550ad398cd01e8456892025-01-24T07:13:23ZengFrontiers Media S.A.Frontiers in Natural Products2813-26022025-01-01310.3389/fntpr.2024.15325431532543MATRIX: a platform for cultivation profiling and bioactive discovery in natural product researchZeinab G. KhalilThulasi SritharanAngela A. SalimRobert J. CaponThe MATRIX protocol presents a novel miniaturized 24-well plate approach for microbial cultivation profiling, enabling the efficient and parallel assessment of microbial metabolite production. This method integrates a media MATRIX within a microbioreactor to facilitate the creation of diverse growth conditions for microbial cultivation. The 24-well format supports the simultaneous study of multiple microbial strains and conditions, allowing the discovery of novel bioactive compounds. By employing a variety of media compositions and cultivations, MATRIX enhances the understanding of microbial metabolic pathways and interactions. This protocol provides a robust platform for natural product discovery, microbial ecology studies, and drug development, showcasing its potential to uncover new natural products and other valuable bioactives. The MATRIX approach offers a resource-efficient platform for microbial biodiscovery, contributing significantly to natural product research and drug development.https://www.frontiersin.org/articles/10.3389/fntpr.2024.1532543/fullmatrix methodologymicrobial cultivation profilingnatural product discoverybioactive compoundsbiosynthetic gene clustershigh-throughput screening |
| spellingShingle | Zeinab G. Khalil Thulasi Sritharan Angela A. Salim Robert J. Capon MATRIX: a platform for cultivation profiling and bioactive discovery in natural product research Frontiers in Natural Products matrix methodology microbial cultivation profiling natural product discovery bioactive compounds biosynthetic gene clusters high-throughput screening |
| title | MATRIX: a platform for cultivation profiling and bioactive discovery in natural product research |
| title_full | MATRIX: a platform for cultivation profiling and bioactive discovery in natural product research |
| title_fullStr | MATRIX: a platform for cultivation profiling and bioactive discovery in natural product research |
| title_full_unstemmed | MATRIX: a platform for cultivation profiling and bioactive discovery in natural product research |
| title_short | MATRIX: a platform for cultivation profiling and bioactive discovery in natural product research |
| title_sort | matrix a platform for cultivation profiling and bioactive discovery in natural product research |
| topic | matrix methodology microbial cultivation profiling natural product discovery bioactive compounds biosynthetic gene clusters high-throughput screening |
| url | https://www.frontiersin.org/articles/10.3389/fntpr.2024.1532543/full |
| work_keys_str_mv | AT zeinabgkhalil matrixaplatformforcultivationprofilingandbioactivediscoveryinnaturalproductresearch AT thulasisritharan matrixaplatformforcultivationprofilingandbioactivediscoveryinnaturalproductresearch AT angelaasalim matrixaplatformforcultivationprofilingandbioactivediscoveryinnaturalproductresearch AT robertjcapon matrixaplatformforcultivationprofilingandbioactivediscoveryinnaturalproductresearch |