Genome-Wide Identification and Characterization of Basic Pentacysteine Transcription Factors in <i>Brassica napus</i>
BARLEY B-RECOMBINANT/BASIC PENTACYSTEINE (BBR/BPC), a plant-specific transcription factor family, is a group of GAGA_motif binding factors controlling multiple developmental processes of growth and response to abiotic stresses. BPCs recruit histone remodeling factors for transcriptional repression o...
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2025-04-01
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| author | Huan Hu Yuqin Jiang Chiyuan Liu Ying Zhang Mingxun Chen Zijin Liu |
| author_facet | Huan Hu Yuqin Jiang Chiyuan Liu Ying Zhang Mingxun Chen Zijin Liu |
| author_sort | Huan Hu |
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| description | BARLEY B-RECOMBINANT/BASIC PENTACYSTEINE (BBR/BPC), a plant-specific transcription factor family, is a group of GAGA_motif binding factors controlling multiple developmental processes of growth and response to abiotic stresses. BPCs recruit histone remodeling factors for transcriptional repression of downstream targets. However, the information about BnaBPCs from <i>Brassica napus</i> remains unclear. Here, we identified 25 <i>BnaBPC</i> genes that were mainly localized in the nucleus, randomly localized on 16 chromosomes, and grouped into three subfamilies based on phylogenetic analysis. Twenty-five <i>BnaBPC</i> genes exhibit syntenic relationships with <i>AtBPC</i> genes, and the polypeptides encoded by <i>BnaBPC</i> genes within the same subfamily share similar conserved motifs and protein domains. The expansion of <i>BnaBPC</i> genes underwent whole-genome duplication events and purifying selection in genomes, and all the <i>BnaBPC</i> genes had the same conserved GAGA binding domains. Additionally, the promoter of each <i>BnaBPC</i> gene consisted of various <i>cis</i>-elements associated with stresses, phytohormones, and growth and development. Notably, the seed-specific regulatory element was found only in the <i>BnaC04.BPC4</i> promoter. Further expression pattern analysis showed that <i>BnaBPC</i> members are widely expressed in stems, buds, developing seeds and siliques. These findings provide insights into <i>BnaBPC</i> genes and enrich our understanding of their functional characterization in <i>B. napus</i>. |
| format | Article |
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| institution | DOAJ |
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| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-6ed40d6e03a24ca1afe0002ef3cff16c2025-08-20T03:03:27ZengMDPI AGPlants2223-77472025-04-01147113610.3390/plants14071136Genome-Wide Identification and Characterization of Basic Pentacysteine Transcription Factors in <i>Brassica napus</i>Huan Hu0Yuqin Jiang1Chiyuan Liu2Ying Zhang3Mingxun Chen4Zijin Liu5Shaanxi Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaDepartment of Ecological and Environmental Engineering, Yangling Vocational & Technical College, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaBARLEY B-RECOMBINANT/BASIC PENTACYSTEINE (BBR/BPC), a plant-specific transcription factor family, is a group of GAGA_motif binding factors controlling multiple developmental processes of growth and response to abiotic stresses. BPCs recruit histone remodeling factors for transcriptional repression of downstream targets. However, the information about BnaBPCs from <i>Brassica napus</i> remains unclear. Here, we identified 25 <i>BnaBPC</i> genes that were mainly localized in the nucleus, randomly localized on 16 chromosomes, and grouped into three subfamilies based on phylogenetic analysis. Twenty-five <i>BnaBPC</i> genes exhibit syntenic relationships with <i>AtBPC</i> genes, and the polypeptides encoded by <i>BnaBPC</i> genes within the same subfamily share similar conserved motifs and protein domains. The expansion of <i>BnaBPC</i> genes underwent whole-genome duplication events and purifying selection in genomes, and all the <i>BnaBPC</i> genes had the same conserved GAGA binding domains. Additionally, the promoter of each <i>BnaBPC</i> gene consisted of various <i>cis</i>-elements associated with stresses, phytohormones, and growth and development. Notably, the seed-specific regulatory element was found only in the <i>BnaC04.BPC4</i> promoter. Further expression pattern analysis showed that <i>BnaBPC</i> members are widely expressed in stems, buds, developing seeds and siliques. These findings provide insights into <i>BnaBPC</i> genes and enrich our understanding of their functional characterization in <i>B. napus</i>.https://www.mdpi.com/2223-7747/14/7/1136gene family<i>Brassica napus</i>BPCgenome-wide identificationexpression pattern |
| spellingShingle | Huan Hu Yuqin Jiang Chiyuan Liu Ying Zhang Mingxun Chen Zijin Liu Genome-Wide Identification and Characterization of Basic Pentacysteine Transcription Factors in <i>Brassica napus</i> Plants gene family <i>Brassica napus</i> BPC genome-wide identification expression pattern |
| title | Genome-Wide Identification and Characterization of Basic Pentacysteine Transcription Factors in <i>Brassica napus</i> |
| title_full | Genome-Wide Identification and Characterization of Basic Pentacysteine Transcription Factors in <i>Brassica napus</i> |
| title_fullStr | Genome-Wide Identification and Characterization of Basic Pentacysteine Transcription Factors in <i>Brassica napus</i> |
| title_full_unstemmed | Genome-Wide Identification and Characterization of Basic Pentacysteine Transcription Factors in <i>Brassica napus</i> |
| title_short | Genome-Wide Identification and Characterization of Basic Pentacysteine Transcription Factors in <i>Brassica napus</i> |
| title_sort | genome wide identification and characterization of basic pentacysteine transcription factors in i brassica napus i |
| topic | gene family <i>Brassica napus</i> BPC genome-wide identification expression pattern |
| url | https://www.mdpi.com/2223-7747/14/7/1136 |
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