Analysis of differential gene expression in the brain tissue transcriptome of Jiangkou radish piglets infected with porcine pseudorabies virus
Background: The research focused on the effects of pseudorabies virus on gene expression in piglet brain tissue. Objectives: The goal was to understand the changes in gene expression in piglet brains due to pseudorabies virus infection. Design: The study used a comparative approach with infected and...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-03-01
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| Series: | SAGE Open Medicine |
| Online Access: | https://doi.org/10.1177/20503121251326763 |
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| author | Wei Sun Shan-Shan Liu Yu Fan Sheng-Qing Deng Hua-Qi Zhang Fengzhao Zhu Shi-He Long Tao-Hua Ren Ling Bai |
| author_facet | Wei Sun Shan-Shan Liu Yu Fan Sheng-Qing Deng Hua-Qi Zhang Fengzhao Zhu Shi-He Long Tao-Hua Ren Ling Bai |
| author_sort | Wei Sun |
| collection | DOAJ |
| description | Background: The research focused on the effects of pseudorabies virus on gene expression in piglet brain tissue. Objectives: The goal was to understand the changes in gene expression in piglet brains due to pseudorabies virus infection. Design: The study used a comparative approach with infected and control piglet groups. Methods: Twelve 2-month-old piglets were divided into pseudorabies virus-infected and PBS-treated control groups, with brain tissue analyzed after 7 days. Results: Infected piglets showed increased oligodendrocyte counts and pseudorabies virus-positive signals. Transcriptomic analysis revealed 269 differentially expressed genes, with 149 up-regulated and 120 down-regulated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated these genes are involved in signal transduction, transmembrane transport, apoptosis, and neuroactive ligand–receptor interaction. Quantitative fluorescent PCR validated these findings, particularly for genes related to neuroactive pathways, ferroptosis, and IL-17 signaling. Conclusion: The study provides valuable insights into the molecular alterations caused by pseudorabies virus in piglet brain tissue, enhancing our understanding of pseudorabies virus’s pathogenic mechanisms. |
| format | Article |
| id | doaj-art-6ed2ec8b68534413b210bab92ca104eb |
| institution | OA Journals |
| issn | 2050-3121 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | SAGE Open Medicine |
| spelling | doaj-art-6ed2ec8b68534413b210bab92ca104eb2025-08-20T02:06:35ZengSAGE PublishingSAGE Open Medicine2050-31212025-03-011310.1177/20503121251326763Analysis of differential gene expression in the brain tissue transcriptome of Jiangkou radish piglets infected with porcine pseudorabies virusWei Sun0Shan-Shan Liu1Yu Fan2Sheng-Qing Deng3Hua-Qi Zhang4Fengzhao Zhu5Shi-He Long6Tao-Hua Ren7Ling Bai8Tongren Polytechnic College, Guizhou, ChinaTongren Polytechnic College, Guizhou, ChinaTongren Polytechnic College, Guizhou, ChinaTongren Polytechnic College, Guizhou, ChinaTongren Polytechnic College, Guizhou, ChinaTongren Polytechnic College, Guizhou, ChinaSongtao Miao Autonomous County Agriculture and Rural Bureau, Guizhou, ChinaSongtao Miao Autonomous County Agriculture and Rural Bureau, Guizhou, ChinaHuaihua Vocational and Technical College, Hunan, ChinaBackground: The research focused on the effects of pseudorabies virus on gene expression in piglet brain tissue. Objectives: The goal was to understand the changes in gene expression in piglet brains due to pseudorabies virus infection. Design: The study used a comparative approach with infected and control piglet groups. Methods: Twelve 2-month-old piglets were divided into pseudorabies virus-infected and PBS-treated control groups, with brain tissue analyzed after 7 days. Results: Infected piglets showed increased oligodendrocyte counts and pseudorabies virus-positive signals. Transcriptomic analysis revealed 269 differentially expressed genes, with 149 up-regulated and 120 down-regulated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated these genes are involved in signal transduction, transmembrane transport, apoptosis, and neuroactive ligand–receptor interaction. Quantitative fluorescent PCR validated these findings, particularly for genes related to neuroactive pathways, ferroptosis, and IL-17 signaling. Conclusion: The study provides valuable insights into the molecular alterations caused by pseudorabies virus in piglet brain tissue, enhancing our understanding of pseudorabies virus’s pathogenic mechanisms.https://doi.org/10.1177/20503121251326763 |
| spellingShingle | Wei Sun Shan-Shan Liu Yu Fan Sheng-Qing Deng Hua-Qi Zhang Fengzhao Zhu Shi-He Long Tao-Hua Ren Ling Bai Analysis of differential gene expression in the brain tissue transcriptome of Jiangkou radish piglets infected with porcine pseudorabies virus SAGE Open Medicine |
| title | Analysis of differential gene expression in the brain tissue transcriptome of Jiangkou radish piglets infected with porcine pseudorabies virus |
| title_full | Analysis of differential gene expression in the brain tissue transcriptome of Jiangkou radish piglets infected with porcine pseudorabies virus |
| title_fullStr | Analysis of differential gene expression in the brain tissue transcriptome of Jiangkou radish piglets infected with porcine pseudorabies virus |
| title_full_unstemmed | Analysis of differential gene expression in the brain tissue transcriptome of Jiangkou radish piglets infected with porcine pseudorabies virus |
| title_short | Analysis of differential gene expression in the brain tissue transcriptome of Jiangkou radish piglets infected with porcine pseudorabies virus |
| title_sort | analysis of differential gene expression in the brain tissue transcriptome of jiangkou radish piglets infected with porcine pseudorabies virus |
| url | https://doi.org/10.1177/20503121251326763 |
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