Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions
Low back pain (LBP) is a major physical and socioeconomic challenge worldwide. Nucleus pulposus (NP) is directly associated with LBP due to intervertebral disc (IVD) degeneration. IVD degeneration is mainly caused by structural and matrix-related changes within the IVD occurring during aging and deg...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/8882549 |
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| author | Arjun Sinkemani Feng Wang Zhiyang Xie Lu Chen Cong Zhang Xiaotao Wu |
| author_facet | Arjun Sinkemani Feng Wang Zhiyang Xie Lu Chen Cong Zhang Xiaotao Wu |
| author_sort | Arjun Sinkemani |
| collection | DOAJ |
| description | Low back pain (LBP) is a major physical and socioeconomic challenge worldwide. Nucleus pulposus (NP) is directly associated with LBP due to intervertebral disc (IVD) degeneration. IVD degeneration is mainly caused by structural and matrix-related changes within the IVD occurring during aging and degeneration. Mesenchymal stem cells (MSCs) can differentiate into multiple mesenchymal lineages under specific stimulatory conditions. This study is aimed at evaluating the effectiveness of the nucleus pulposus cell (NPC) conditioned medium for promoting the expression of MSCs and at confirming the expression of healthy NP phenotypic markers recently recommended by the Spine Research Interest Group. Expression was investigated using quantitative polymerase chain reaction (qPCR) and western blotting under normoxic and hypoxic conditions. qPCR and western blotting demonstrated significant upregulation of NP marker expression in MSCs cultured under hypoxic conditions and treated with the 50% or 100% NPC conditioned medium, compared with those cultured under normoxic conditions. Upregulation was highest in the presence of the 100% NPC conditioned medium compared with the control group (aggrecan, p<0.01; brachyury, p<0.05; collagen II, p<0.001; KRT8, p<0.01; KRT19, p<0.001; and Shh, p<0.01). The expression levels of genes in MSCs treated with the 50% NPC conditioned medium also showed upregulation compared with the control group (collagen II, p<0.05; KRT8, p<0.05; and KRT19, p<0.01). These findings suggested that the NPC conditioned medium stimulated MSC differentiation into an NP-like phenotype with distinct characteristics. The results could inform strategies for IVD regeneration. |
| format | Article |
| id | doaj-art-6ecd1f0054bf43dfab56ee696982fe60 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6ecd1f0054bf43dfab56ee696982fe602025-02-03T00:58:52ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/88825498882549Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic ConditionsArjun Sinkemani0Feng Wang1Zhiyang Xie2Lu Chen3Cong Zhang4Xiaotao Wu5Department of Spine Surgery, Zhongda Hospital, Southeast University School of Medicine, 87# Dingjiaqiao Road, 210009 Nanjing, ChinaDepartment of Spine Surgery, Zhongda Hospital, Southeast University School of Medicine, 87# Dingjiaqiao Road, 210009 Nanjing, ChinaDepartment of Spine Surgery, Zhongda Hospital, Southeast University School of Medicine, 87# Dingjiaqiao Road, 210009 Nanjing, ChinaDepartment of Spine Surgery, Zhongda Hospital, Southeast University School of Medicine, 87# Dingjiaqiao Road, 210009 Nanjing, ChinaDepartment of Spine Surgery, Zhongda Hospital, Southeast University School of Medicine, 87# Dingjiaqiao Road, 210009 Nanjing, ChinaDepartment of Spine Surgery, Zhongda Hospital, Southeast University School of Medicine, 87# Dingjiaqiao Road, 210009 Nanjing, ChinaLow back pain (LBP) is a major physical and socioeconomic challenge worldwide. Nucleus pulposus (NP) is directly associated with LBP due to intervertebral disc (IVD) degeneration. IVD degeneration is mainly caused by structural and matrix-related changes within the IVD occurring during aging and degeneration. Mesenchymal stem cells (MSCs) can differentiate into multiple mesenchymal lineages under specific stimulatory conditions. This study is aimed at evaluating the effectiveness of the nucleus pulposus cell (NPC) conditioned medium for promoting the expression of MSCs and at confirming the expression of healthy NP phenotypic markers recently recommended by the Spine Research Interest Group. Expression was investigated using quantitative polymerase chain reaction (qPCR) and western blotting under normoxic and hypoxic conditions. qPCR and western blotting demonstrated significant upregulation of NP marker expression in MSCs cultured under hypoxic conditions and treated with the 50% or 100% NPC conditioned medium, compared with those cultured under normoxic conditions. Upregulation was highest in the presence of the 100% NPC conditioned medium compared with the control group (aggrecan, p<0.01; brachyury, p<0.05; collagen II, p<0.001; KRT8, p<0.01; KRT19, p<0.001; and Shh, p<0.01). The expression levels of genes in MSCs treated with the 50% NPC conditioned medium also showed upregulation compared with the control group (collagen II, p<0.05; KRT8, p<0.05; and KRT19, p<0.01). These findings suggested that the NPC conditioned medium stimulated MSC differentiation into an NP-like phenotype with distinct characteristics. The results could inform strategies for IVD regeneration.http://dx.doi.org/10.1155/2020/8882549 |
| spellingShingle | Arjun Sinkemani Feng Wang Zhiyang Xie Lu Chen Cong Zhang Xiaotao Wu Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions Stem Cells International |
| title | Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions |
| title_full | Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions |
| title_fullStr | Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions |
| title_full_unstemmed | Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions |
| title_short | Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions |
| title_sort | nucleus pulposus cell conditioned medium promotes mesenchymal stem cell differentiation into nucleus pulposus like cells under hypoxic conditions |
| url | http://dx.doi.org/10.1155/2020/8882549 |
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