Detoxification Effect of Selenium: Alleviating the Inhibition of Cadmium Stress on the Growth of Rape (<i>Brassica napus</i> L.) by Regulating Photosynthetic Pigments, Metal Complexation Reaction, and Antioxidant System

Cadmium is considered a highly toxic metallic element that does not have any beneficial biological functions for humans or plants. It has been reported that the antagonism of selenium to heavy metal stress has been observed in a variety of plants, and appropriate selenium could alleviate heavy-metal...

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Main Authors: Cixing He, Yuanyuan Zhao, Hua Zhang, Zhen Wang, Jiliang Lü, Liqiang Ge, Xiaohu Zhao, Chao Xu
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/15/3/541
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author Cixing He
Yuanyuan Zhao
Hua Zhang
Zhen Wang
Jiliang Lü
Liqiang Ge
Xiaohu Zhao
Chao Xu
author_facet Cixing He
Yuanyuan Zhao
Hua Zhang
Zhen Wang
Jiliang Lü
Liqiang Ge
Xiaohu Zhao
Chao Xu
author_sort Cixing He
collection DOAJ
description Cadmium is considered a highly toxic metallic element that does not have any beneficial biological functions for humans or plants. It has been reported that the antagonism of selenium to heavy metal stress has been observed in a variety of plants, and appropriate selenium could alleviate heavy-metal-induced oxidative damage and reduce the accumulation of heavy metals in plants. Changes in physiological characteristics, root tip cells, cadmium concentration, and accumulation of rape under cadmium stress were investigated in this study through pot experiment. Results showed that selenium could alleviate the inhibitory effect of cadmium on the growth of rape seedlings. The concentration and accumulation of cadmium were decreased after the selenium application in rape seeds, ranging from 19.93 to 22.97% and 27.96 to 43.88%, respectively, and the decrease in photosynthetic pigment content induced by cadmium was significantly improved. The results of transmission electron microscopy showed that exogenous selenium and cadmium had metal complexation reaction and formed black precipitation, which may be related to the detoxification effect of selenium on cadmium. More critically, with the addition of selenium, the plasma membrane damage and free radical accumulation in root tips induced by cadmium stress were gradually alleviated in the histochemical staining experiment of rape root tips. These results may provide evidence for exploring effective measures to reduce cadmium accumulation in rape under cadmium-contaminated areas.
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institution Kabale University
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series Agronomy
spelling doaj-art-6ecb9c5a8a4f45c7ada5dd27048b19842025-08-20T03:40:42ZengMDPI AGAgronomy2073-43952025-02-0115354110.3390/agronomy15030541Detoxification Effect of Selenium: Alleviating the Inhibition of Cadmium Stress on the Growth of Rape (<i>Brassica napus</i> L.) by Regulating Photosynthetic Pigments, Metal Complexation Reaction, and Antioxidant SystemCixing He0Yuanyuan Zhao1Hua Zhang2Zhen Wang3Jiliang Lü4Liqiang Ge5Xiaohu Zhao6Chao Xu7Xinjiang Key Laboratory of Soil and Plant Ecological Processes, College of Resource and Environment, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Resources and Environment, Huazhong Agricultural University, Research Center of Trace Elements, Wuhan 430070, ChinaState Key Laboratory of Environmental Geochemistry, Guiyang 550081, ChinaSchool of Environmental Science and Engineering, Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, Hubei Polytechnic University, Huangshi 435003, ChinaSchool of Environmental Science and Engineering, Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, Hubei Polytechnic University, Huangshi 435003, ChinaNational Research Center for Geoanalysis, Key Laboratory of Eco-geochemistry, Ministry of Natural Resources, Beijing 100037, ChinaXinjiang Key Laboratory of Soil and Plant Ecological Processes, College of Resource and Environment, Xinjiang Agricultural University, Urumqi 830052, ChinaXinjiang Key Laboratory of Soil and Plant Ecological Processes, College of Resource and Environment, Xinjiang Agricultural University, Urumqi 830052, ChinaCadmium is considered a highly toxic metallic element that does not have any beneficial biological functions for humans or plants. It has been reported that the antagonism of selenium to heavy metal stress has been observed in a variety of plants, and appropriate selenium could alleviate heavy-metal-induced oxidative damage and reduce the accumulation of heavy metals in plants. Changes in physiological characteristics, root tip cells, cadmium concentration, and accumulation of rape under cadmium stress were investigated in this study through pot experiment. Results showed that selenium could alleviate the inhibitory effect of cadmium on the growth of rape seedlings. The concentration and accumulation of cadmium were decreased after the selenium application in rape seeds, ranging from 19.93 to 22.97% and 27.96 to 43.88%, respectively, and the decrease in photosynthetic pigment content induced by cadmium was significantly improved. The results of transmission electron microscopy showed that exogenous selenium and cadmium had metal complexation reaction and formed black precipitation, which may be related to the detoxification effect of selenium on cadmium. More critically, with the addition of selenium, the plasma membrane damage and free radical accumulation in root tips induced by cadmium stress were gradually alleviated in the histochemical staining experiment of rape root tips. These results may provide evidence for exploring effective measures to reduce cadmium accumulation in rape under cadmium-contaminated areas.https://www.mdpi.com/2073-4395/15/3/541cadmiumselenium<i>Brassica napus</i> L.photosynthetic pigmentroot tipsreactive oxygen species
spellingShingle Cixing He
Yuanyuan Zhao
Hua Zhang
Zhen Wang
Jiliang Lü
Liqiang Ge
Xiaohu Zhao
Chao Xu
Detoxification Effect of Selenium: Alleviating the Inhibition of Cadmium Stress on the Growth of Rape (<i>Brassica napus</i> L.) by Regulating Photosynthetic Pigments, Metal Complexation Reaction, and Antioxidant System
Agronomy
cadmium
selenium
<i>Brassica napus</i> L.
photosynthetic pigment
root tips
reactive oxygen species
title Detoxification Effect of Selenium: Alleviating the Inhibition of Cadmium Stress on the Growth of Rape (<i>Brassica napus</i> L.) by Regulating Photosynthetic Pigments, Metal Complexation Reaction, and Antioxidant System
title_full Detoxification Effect of Selenium: Alleviating the Inhibition of Cadmium Stress on the Growth of Rape (<i>Brassica napus</i> L.) by Regulating Photosynthetic Pigments, Metal Complexation Reaction, and Antioxidant System
title_fullStr Detoxification Effect of Selenium: Alleviating the Inhibition of Cadmium Stress on the Growth of Rape (<i>Brassica napus</i> L.) by Regulating Photosynthetic Pigments, Metal Complexation Reaction, and Antioxidant System
title_full_unstemmed Detoxification Effect of Selenium: Alleviating the Inhibition of Cadmium Stress on the Growth of Rape (<i>Brassica napus</i> L.) by Regulating Photosynthetic Pigments, Metal Complexation Reaction, and Antioxidant System
title_short Detoxification Effect of Selenium: Alleviating the Inhibition of Cadmium Stress on the Growth of Rape (<i>Brassica napus</i> L.) by Regulating Photosynthetic Pigments, Metal Complexation Reaction, and Antioxidant System
title_sort detoxification effect of selenium alleviating the inhibition of cadmium stress on the growth of rape i brassica napus i l by regulating photosynthetic pigments metal complexation reaction and antioxidant system
topic cadmium
selenium
<i>Brassica napus</i> L.
photosynthetic pigment
root tips
reactive oxygen species
url https://www.mdpi.com/2073-4395/15/3/541
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