Modulating ion channels with nanobodies
Ion channels play instrumental roles in regulating membrane potential and cross-membrane signal transduction, thus making them attractive targets for understanding various physiological processes and associated diseases. Gaining a deeper understanding of their structural and functional properties ha...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Synthetic and Systems Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X25000134 |
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| author | Sher Ali Ashley Suris Yun Huang Yubin Zhou |
| author_facet | Sher Ali Ashley Suris Yun Huang Yubin Zhou |
| author_sort | Sher Ali |
| collection | DOAJ |
| description | Ion channels play instrumental roles in regulating membrane potential and cross-membrane signal transduction, thus making them attractive targets for understanding various physiological processes and associated diseases. Gaining a deeper understanding of their structural and functional properties has significant implications for developing therapeutic interventions. In recent years, nanobodies, single-domain antibody fragments derived from camelids, have emerged as powerful tools in ion channel and synthetic biology research. Their small size, high specificity, and ability to recognize difficult-to-reach epitopes offer advantages over conventional antibodies and biologics. Furthermore, their resemblance to the variable region of human IgG family III reduces immunogenicity concerns. Nanobodies have introduced new opportunities for exploring ion channel structure-function relationships and offer a promising alternative to conventional drugs, which often face challenges such as off-target effects and toxicity. This review highlights recent progress in applying nanobodies to interrogate and modulate ion channel activity, with an emphasis on their potential to overcome current technical and therapeutic limitations. |
| format | Article |
| id | doaj-art-6ecae6bf47624e0682e4973e0cf4d8dd |
| institution | OA Journals |
| issn | 2405-805X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj-art-6ecae6bf47624e0682e4973e0cf4d8dd2025-08-20T02:36:19ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2025-06-0110259359910.1016/j.synbio.2025.02.005Modulating ion channels with nanobodiesSher Ali0Ashley Suris1Yun Huang2Yubin Zhou3Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USACenter for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USACenter for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA; Department of Translational Medical Sciences, College of Medicine, Texas A&M University, Houston, TX 77030, USACenter for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA; Department of Translational Medical Sciences, College of Medicine, Texas A&M University, Houston, TX 77030, USA; Corresponding author. Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX, 77030, USAIon channels play instrumental roles in regulating membrane potential and cross-membrane signal transduction, thus making them attractive targets for understanding various physiological processes and associated diseases. Gaining a deeper understanding of their structural and functional properties has significant implications for developing therapeutic interventions. In recent years, nanobodies, single-domain antibody fragments derived from camelids, have emerged as powerful tools in ion channel and synthetic biology research. Their small size, high specificity, and ability to recognize difficult-to-reach epitopes offer advantages over conventional antibodies and biologics. Furthermore, their resemblance to the variable region of human IgG family III reduces immunogenicity concerns. Nanobodies have introduced new opportunities for exploring ion channel structure-function relationships and offer a promising alternative to conventional drugs, which often face challenges such as off-target effects and toxicity. This review highlights recent progress in applying nanobodies to interrogate and modulate ion channel activity, with an emphasis on their potential to overcome current technical and therapeutic limitations.http://www.sciencedirect.com/science/article/pii/S2405805X25000134NanobodyAntibody engineeringIon channelsImmunotherapySynthetic biologyTherapeutics |
| spellingShingle | Sher Ali Ashley Suris Yun Huang Yubin Zhou Modulating ion channels with nanobodies Synthetic and Systems Biotechnology Nanobody Antibody engineering Ion channels Immunotherapy Synthetic biology Therapeutics |
| title | Modulating ion channels with nanobodies |
| title_full | Modulating ion channels with nanobodies |
| title_fullStr | Modulating ion channels with nanobodies |
| title_full_unstemmed | Modulating ion channels with nanobodies |
| title_short | Modulating ion channels with nanobodies |
| title_sort | modulating ion channels with nanobodies |
| topic | Nanobody Antibody engineering Ion channels Immunotherapy Synthetic biology Therapeutics |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X25000134 |
| work_keys_str_mv | AT sherali modulatingionchannelswithnanobodies AT ashleysuris modulatingionchannelswithnanobodies AT yunhuang modulatingionchannelswithnanobodies AT yubinzhou modulatingionchannelswithnanobodies |