Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae)
In the U.S., Helicoverpa zea (Boddie) is a major pest targeted by both transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins. Resistance of insect to Bt maize and cotton containing cry1A and cry2A genes has widely occurred in the U.S. In this study, two trials were performed to...
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KeAi Communications Co., Ltd.
2023-07-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922002490 |
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| author | Tiago SILVA Ying NIU Tyler TOWLES Sebe BROWN Graham P. HEAD Wade WALKER Fangneng HUANG |
| author_facet | Tiago SILVA Ying NIU Tyler TOWLES Sebe BROWN Graham P. HEAD Wade WALKER Fangneng HUANG |
| author_sort | Tiago SILVA |
| collection | DOAJ |
| description | In the U.S., Helicoverpa zea (Boddie) is a major pest targeted by both transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins. Resistance of insect to Bt maize and cotton containing cry1A and cry2A genes has widely occurred in the U.S. In this study, two trials were performed to investigate larval survival and development of a Cry1A.105/Cry2Ab2 dual-protein resistant (VT2P-RR), a susceptible, and an F1 heterozygous (VT2P-RS) populations of H. zea on ears of nine Bt and three non-Bt maize hybrids. The Bt maize hybrids evaluated represent five common pyramided traits expressing two or three of the Cry1A.105, Cry1Ab, Cry1F, Cry2Ab2, and Vip3Aa20 proteins. In the laboratory, neonates of the three H. zea populations were inoculated on silks of ears collected from maize at R1–R2 plant stages; and larval survivorship was checked 10 d after neonate release. All three insect populations survived normally on non-Bt maize ears. Varied numbers of VT2P-RR and VT2P-RS survived on ears of Cry1A.105/Cry2Ab2 maize, while all larvae of the three populations died or could not develop on ears of Vip3Aa20-expressing maize. The results demonstrated that the dual-protein resistant H. zea was not cross-resistant to Vip3Aa20-expressing maize, and thus traits with vip3Aa20 gene should be effective to manage Cry1A.105/Cry2Ab2-resistant H. zea. The resistance in VT2P-RR was determined to be incomplete on Cry1A.105/Cry2Ab2 maize. The effective dominance levels varied greatly, from recessive to incompletely dominant, depending on maize hybrids and trials, suggesting that proper selection of maize hybrids could be important for mitigating the Cry1A.105/Cry2Ab2 resistance. The data generated should aid in modeling multiple-protein Bt resistance in H. zea. |
| format | Article |
| id | doaj-art-6eb2d7baef8d457aad88c0a2558f27e5 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Journal of Integrative Agriculture |
| spelling | doaj-art-6eb2d7baef8d457aad88c0a2558f27e52025-08-20T03:56:18ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-07-012272151216110.1016/j.jia.2022.09.024Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae)Tiago SILVA0Ying NIU1Tyler TOWLES2Sebe BROWN3Graham P. HEAD4Wade WALKER5Fangneng HUANG6Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70817, USADepartment of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70817, USAMacon Ridge Research Station, Louisiana State University Agricultural Center, Winnsboro, Louisiana 71295, USADean Research Station, Louisiana State University Agricultural Center, Alexandria, Louisiana 71302, USABayer Crop Science, Chesterfield, Missouri 63017, USAMacon Ridge Research Station, Louisiana State University Agricultural Center, Winnsboro, Louisiana 71295, USADepartment of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70817, USA; Correspondence Fangneng HUANGIn the U.S., Helicoverpa zea (Boddie) is a major pest targeted by both transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins. Resistance of insect to Bt maize and cotton containing cry1A and cry2A genes has widely occurred in the U.S. In this study, two trials were performed to investigate larval survival and development of a Cry1A.105/Cry2Ab2 dual-protein resistant (VT2P-RR), a susceptible, and an F1 heterozygous (VT2P-RS) populations of H. zea on ears of nine Bt and three non-Bt maize hybrids. The Bt maize hybrids evaluated represent five common pyramided traits expressing two or three of the Cry1A.105, Cry1Ab, Cry1F, Cry2Ab2, and Vip3Aa20 proteins. In the laboratory, neonates of the three H. zea populations were inoculated on silks of ears collected from maize at R1–R2 plant stages; and larval survivorship was checked 10 d after neonate release. All three insect populations survived normally on non-Bt maize ears. Varied numbers of VT2P-RR and VT2P-RS survived on ears of Cry1A.105/Cry2Ab2 maize, while all larvae of the three populations died or could not develop on ears of Vip3Aa20-expressing maize. The results demonstrated that the dual-protein resistant H. zea was not cross-resistant to Vip3Aa20-expressing maize, and thus traits with vip3Aa20 gene should be effective to manage Cry1A.105/Cry2Ab2-resistant H. zea. The resistance in VT2P-RR was determined to be incomplete on Cry1A.105/Cry2Ab2 maize. The effective dominance levels varied greatly, from recessive to incompletely dominant, depending on maize hybrids and trials, suggesting that proper selection of maize hybrids could be important for mitigating the Cry1A.105/Cry2Ab2 resistance. The data generated should aid in modeling multiple-protein Bt resistance in H. zea.http://www.sciencedirect.com/science/article/pii/S2095311922002490corn earwormCry1A/Cry2Aeffective dominanceincomplete resistanceBt maize resistance management |
| spellingShingle | Tiago SILVA Ying NIU Tyler TOWLES Sebe BROWN Graham P. HEAD Wade WALKER Fangneng HUANG Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) Journal of Integrative Agriculture corn earworm Cry1A/Cry2A effective dominance incomplete resistance Bt maize resistance management |
| title | Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) |
| title_full | Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) |
| title_fullStr | Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) |
| title_full_unstemmed | Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) |
| title_short | Selection, effective dominance, and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) |
| title_sort | selection effective dominance and completeness of cry1a 105 cry2ab2 dual protein resistance in helicoverpa zea boddie lepidoptera noctuidae |
| topic | corn earworm Cry1A/Cry2A effective dominance incomplete resistance Bt maize resistance management |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922002490 |
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