Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor
The WHO has classified Helicobacter pylori as a group 1 carcinogen for stomach cancer since early 1994. However, despite the high prevalence of Helicobacter pylori infection, only about 3% of infected people eventually develop gastric cancer.Biomolecular detections of Helicobacter pylori(HP) were co...
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MDPI AG
2024-12-01
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| author | Kyung Lee Sihyun Jun Yeseul Oh Seojun Lee Ye Jun Oh Keum Sook Kim Suw Young Ly |
| author_facet | Kyung Lee Sihyun Jun Yeseul Oh Seojun Lee Ye Jun Oh Keum Sook Kim Suw Young Ly |
| author_sort | Kyung Lee |
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| description | The WHO has classified Helicobacter pylori as a group 1 carcinogen for stomach cancer since early 1994. However, despite the high prevalence of Helicobacter pylori infection, only about 3% of infected people eventually develop gastric cancer.Biomolecular detections of Helicobacter pylori(HP) were compared using specially modified sensors and fluorine immobilized on a carbon nanotube (HFCNT) electrode, which yielded sensitive results. Handheld voltammetric circuits were used for optimization. An anodic voltammogram of HP molecular oxidation was obtained at 0.0 V ± 0.1 (versus the Ag/AgCl/KCl) in a 0.1 ± 0.2 M NH<sub>4</sub>H<sub>2</sub>PO<sub>4</sub> electrolyte solution. Under optimized conditions, the analytical working range was 2.98 × 10<sup>3</sup>–22.127 × 10<sup>−3</sup> CFU/mL HP using square wave (SW) stripping voltammetry, precision of R<sup>2</sup> = 0.9857 ± 0.0005 (SWSV), the detection limit approached to 2.5 × 10<sup>2</sup> CFU/mL HP (S/N = 3).The developed techniques have been applied to diagnosis of early-stage HP infections using stomach tissue from healthy humans and gastric patients. |
| format | Article |
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| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-6eae3f533e6e4db69f3de98818c3a9e92025-08-20T02:43:43ZengMDPI AGMicroorganisms2076-26072024-12-011212253110.3390/microorganisms12122531Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube MicrosensorKyung Lee0Sihyun Jun1Yeseul Oh2Seojun Lee3Ye Jun Oh4Keum Sook Kim5Suw Young Ly6Biosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaUniversity of Michigan College of Pharmacy, 428 Church St, Ann Arbor, MI 48109, USABiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaThe WHO has classified Helicobacter pylori as a group 1 carcinogen for stomach cancer since early 1994. However, despite the high prevalence of Helicobacter pylori infection, only about 3% of infected people eventually develop gastric cancer.Biomolecular detections of Helicobacter pylori(HP) were compared using specially modified sensors and fluorine immobilized on a carbon nanotube (HFCNT) electrode, which yielded sensitive results. Handheld voltammetric circuits were used for optimization. An anodic voltammogram of HP molecular oxidation was obtained at 0.0 V ± 0.1 (versus the Ag/AgCl/KCl) in a 0.1 ± 0.2 M NH<sub>4</sub>H<sub>2</sub>PO<sub>4</sub> electrolyte solution. Under optimized conditions, the analytical working range was 2.98 × 10<sup>3</sup>–22.127 × 10<sup>−3</sup> CFU/mL HP using square wave (SW) stripping voltammetry, precision of R<sup>2</sup> = 0.9857 ± 0.0005 (SWSV), the detection limit approached to 2.5 × 10<sup>2</sup> CFU/mL HP (S/N = 3).The developed techniques have been applied to diagnosis of early-stage HP infections using stomach tissue from healthy humans and gastric patients.https://www.mdpi.com/2076-2607/12/12/2531voltammetrystrippinghelicobacter pylorigastric patientscarbon nanotube microsensor |
| spellingShingle | Kyung Lee Sihyun Jun Yeseul Oh Seojun Lee Ye Jun Oh Keum Sook Kim Suw Young Ly Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor Microorganisms voltammetry stripping helicobacter pylori gastric patients carbon nanotube microsensor |
| title | Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor |
| title_full | Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor |
| title_fullStr | Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor |
| title_full_unstemmed | Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor |
| title_short | Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor |
| title_sort | pathological in vivo analysis of helicobacter dna infection in stomach cells using carbon nanotube microsensor |
| topic | voltammetry stripping helicobacter pylori gastric patients carbon nanotube microsensor |
| url | https://www.mdpi.com/2076-2607/12/12/2531 |
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