Pathological In Vivo Analysis of Helicobacter DNA Infection in Stomach Cells Using Carbon Nanotube Microsensor
The WHO has classified Helicobacter pylori as a group 1 carcinogen for stomach cancer since early 1994. However, despite the high prevalence of Helicobacter pylori infection, only about 3% of infected people eventually develop gastric cancer.Biomolecular detections of Helicobacter pylori(HP) were co...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/12/12/2531 |
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| Summary: | The WHO has classified Helicobacter pylori as a group 1 carcinogen for stomach cancer since early 1994. However, despite the high prevalence of Helicobacter pylori infection, only about 3% of infected people eventually develop gastric cancer.Biomolecular detections of Helicobacter pylori(HP) were compared using specially modified sensors and fluorine immobilized on a carbon nanotube (HFCNT) electrode, which yielded sensitive results. Handheld voltammetric circuits were used for optimization. An anodic voltammogram of HP molecular oxidation was obtained at 0.0 V ± 0.1 (versus the Ag/AgCl/KCl) in a 0.1 ± 0.2 M NH<sub>4</sub>H<sub>2</sub>PO<sub>4</sub> electrolyte solution. Under optimized conditions, the analytical working range was 2.98 × 10<sup>3</sup>–22.127 × 10<sup>−3</sup> CFU/mL HP using square wave (SW) stripping voltammetry, precision of R<sup>2</sup> = 0.9857 ± 0.0005 (SWSV), the detection limit approached to 2.5 × 10<sup>2</sup> CFU/mL HP (S/N = 3).The developed techniques have been applied to diagnosis of early-stage HP infections using stomach tissue from healthy humans and gastric patients. |
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| ISSN: | 2076-2607 |