Anti-melanoma activity of green-produced nanosilver-chlorhexidine complex
This study follows the in vitro anti-melanoma activity of silver nanoparticles (Sn) obtained by “green” reduction with catechins from Camellia sinensis and superficially charged with chlorhexidine diacetate (Cx+). The antiproliferative activity was assessed on human keratinocytes (HaCaT) and human m...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pensoft Publishers
2025-01-01
|
| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/143419/download/pdf/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study follows the in vitro anti-melanoma activity of silver nanoparticles (Sn) obtained by “green” reduction with catechins from Camellia sinensis and superficially charged with chlorhexidine diacetate (Cx+). The antiproliferative activity was assessed on human keratinocytes (HaCaT) and human melanoma (SH-4) cell lines. The respective IC50 values and selective indexes (SI) were retrieved for both the non-conjugated and [Cx+]-conjugated nanosilver forms (Sn-Cx+). As a next step, the active Sn-Cx+ complex was formulated into a prototype of an adhesive patch comprised of ammonio methacrylate copolymer type B and hydroxypropyl methylcellulose in a 1:2 ratio. This resulted in a strong anti-tumor effect (IC50 0.759 ± 0.062 µg/mL) with a high selectivity (SI 4.0) exceeding even the bare colloid’s performance that was recorded. The findings of this experiment suggest the potential applicability of the invention in the local chemotherapy of skin melanoma in its early or post-operative stage. |
|---|---|
| ISSN: | 2603-557X |