EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton
EPSPS is a key gene in the shikimic acid synthesis pathway that has been widely used in breeding crops with herbicide resistance. However, its role in regulating cell elongation is poorly understood. Through the overexpression of EPSPS genes, we generated lines resistant to glyphosate that exhibit a...
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KeAi Communications Co., Ltd.
2024-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923003854 |
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| author | Qingdi Yan Wei Hu Chenxu Gao Lan Yang Jiaxian Yang Renju Liu Masum Billah Yongjun Lin Ji Liu Pengfei Miao Zhaoen Yang Fuguang Li Wenqiang Qin |
| author_facet | Qingdi Yan Wei Hu Chenxu Gao Lan Yang Jiaxian Yang Renju Liu Masum Billah Yongjun Lin Ji Liu Pengfei Miao Zhaoen Yang Fuguang Li Wenqiang Qin |
| author_sort | Qingdi Yan |
| collection | DOAJ |
| description | EPSPS is a key gene in the shikimic acid synthesis pathway that has been widely used in breeding crops with herbicide resistance. However, its role in regulating cell elongation is poorly understood. Through the overexpression of EPSPS genes, we generated lines resistant to glyphosate that exhibit an unexpected dwarf phenotype. A representative line, DHR1, exhibits a stable dwarf phenotype throughout its entire growth period. Except for plant height, the other agronomic traits of DHR1 are similar to its transgenic explants ZM24. Paraffin section observations showed that DHR1 internodes are shortened due to reduced elongation and division of the internode cells. Exogenous hormones confirmed that DHR1 is not a classical brassinolide (BR)- or gibberellin (GA)-related dwarfing mutant. Hybridization analysis and fine mapping confirmed that the EPSPS gene is the causal gene for dwarfism, and the phenotype can be inherited in different genotypes. Transcriptome and metabolome analyses showed that genes associated with the phenylpropanoid synthesis pathway are enriched in DHR1 compared with ZM24. Flavonoid metabolites are enriched in DHR1, whereas lignin metabolites are reduced. The enhancement of flavonoids likely results in differential expression of auxin signal pathway genes and alters the auxin response, subsequently affecting cell elongation. This study provides a new strategy for generating dwarfs and will accelerate advancements in light simplification in the cultivation and mechanized harvesting of cotton. |
| format | Article |
| id | doaj-art-6e94ce12060e4172ae013c516a2f4021 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-6e94ce12060e4172ae013c516a2f40212025-08-20T03:56:18ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192024-10-0123103437345610.1016/j.jia.2023.11.002EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cottonQingdi Yan0Wei Hu1Chenxu Gao2Lan Yang3Jiaxian Yang4Renju Liu5Masum Billah6Yongjun Lin7Ji Liu8Pengfei Miao9Zhaoen Yang10Fuguang Li11Wenqiang Qin12State Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University, Zhengzhou 450066, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University, Zhengzhou 450066, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University, Zhengzhou 450066, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University, Zhengzhou 450066, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572025, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572025, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; State Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University, Zhengzhou 450066, China; Correspondence Zhaoen YangState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; State Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University, Zhengzhou 450066, China; Correspondence Fuguang LiState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572025, China; Correspondence Wenqiang QinEPSPS is a key gene in the shikimic acid synthesis pathway that has been widely used in breeding crops with herbicide resistance. However, its role in regulating cell elongation is poorly understood. Through the overexpression of EPSPS genes, we generated lines resistant to glyphosate that exhibit an unexpected dwarf phenotype. A representative line, DHR1, exhibits a stable dwarf phenotype throughout its entire growth period. Except for plant height, the other agronomic traits of DHR1 are similar to its transgenic explants ZM24. Paraffin section observations showed that DHR1 internodes are shortened due to reduced elongation and division of the internode cells. Exogenous hormones confirmed that DHR1 is not a classical brassinolide (BR)- or gibberellin (GA)-related dwarfing mutant. Hybridization analysis and fine mapping confirmed that the EPSPS gene is the causal gene for dwarfism, and the phenotype can be inherited in different genotypes. Transcriptome and metabolome analyses showed that genes associated with the phenylpropanoid synthesis pathway are enriched in DHR1 compared with ZM24. Flavonoid metabolites are enriched in DHR1, whereas lignin metabolites are reduced. The enhancement of flavonoids likely results in differential expression of auxin signal pathway genes and alters the auxin response, subsequently affecting cell elongation. This study provides a new strategy for generating dwarfs and will accelerate advancements in light simplification in the cultivation and mechanized harvesting of cotton.http://www.sciencedirect.com/science/article/pii/S2095311923003854EPSPScottonT-DNAdwarfismherbicide resistance |
| spellingShingle | Qingdi Yan Wei Hu Chenxu Gao Lan Yang Jiaxian Yang Renju Liu Masum Billah Yongjun Lin Ji Liu Pengfei Miao Zhaoen Yang Fuguang Li Wenqiang Qin EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton Journal of Integrative Agriculture EPSPS cotton T-DNA dwarfism herbicide resistance |
| title | EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton |
| title_full | EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton |
| title_fullStr | EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton |
| title_full_unstemmed | EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton |
| title_short | EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton |
| title_sort | epsps regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton |
| topic | EPSPS cotton T-DNA dwarfism herbicide resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923003854 |
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