Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing
Human adipose-derived stem cells (hASCs) are widely used in regenerative medicine due to their accessibility and high proliferative capacity. Platelet lysate (PL) has recently emerged as a promising alternative to fetal bovine serum (FBS), offering superior cell expansion potential; however, the mol...
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MDPI AG
2025-07-01
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| author | Sakurako Kunieda Michika Fukui Atsuyuki Kuro Toshihito Mitsui Huan Li Zhongxin Sun Takayuki Ueda Shigeru Taketani Koichiro Higasa Natsuko Kakudo |
| author_facet | Sakurako Kunieda Michika Fukui Atsuyuki Kuro Toshihito Mitsui Huan Li Zhongxin Sun Takayuki Ueda Shigeru Taketani Koichiro Higasa Natsuko Kakudo |
| author_sort | Sakurako Kunieda |
| collection | DOAJ |
| description | Human adipose-derived stem cells (hASCs) are widely used in regenerative medicine due to their accessibility and high proliferative capacity. Platelet lysate (PL) has recently emerged as a promising alternative to fetal bovine serum (FBS), offering superior cell expansion potential; however, the molecular basis for its efficacy remains insufficiently elucidated. In this study, we performed RNA sequencing to compare hASCs cultured with PL or FBS, revealing a significant upregulation of genes related to stress response and cell proliferation under PL conditions. These findings were validated by RT–qPCR and supported by functional assays demonstrating enhanced cellular resilience to oxidative and genotoxic stress, reduced doxorubicin-induced senescence, and improved antiapoptotic properties. In a murine wound model, PL-treated wounds showed accelerated healing, characterized by thicker dermis-like tissue formation and increased angiogenesis. Immunohistochemical analysis further revealed elevated expression of chk1, a DNA damage response kinase encoded by CHEK1, which plays a central role in maintaining genomic integrity during stress-induced repair. Collectively, these results highlight PL not only as a viable substitute for FBS in hASC expansion but also as a bioactive supplement that enhances regenerative efficacy by promoting proliferation, stress resistance, and antiaging functions. |
| format | Article |
| id | doaj-art-6e850a6f459d41349cbda68261d20d21 |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj-art-6e850a6f459d41349cbda68261d20d212025-08-20T03:36:03ZengMDPI AGCells2073-44092025-07-011415115410.3390/cells14151154Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound HealingSakurako Kunieda0Michika Fukui1Atsuyuki Kuro2Toshihito Mitsui3Huan Li4Zhongxin Sun5Takayuki Ueda6Shigeru Taketani7Koichiro Higasa8Natsuko Kakudo9Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Kansai Medical University Medical Center, 10-15 Fumizono-cho, Moriguchi 570-8507, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanDepartment of Genome Analysis, Institute of Biomedical Science, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanDepartment of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata 573-1010, Osaka, JapanHuman adipose-derived stem cells (hASCs) are widely used in regenerative medicine due to their accessibility and high proliferative capacity. Platelet lysate (PL) has recently emerged as a promising alternative to fetal bovine serum (FBS), offering superior cell expansion potential; however, the molecular basis for its efficacy remains insufficiently elucidated. In this study, we performed RNA sequencing to compare hASCs cultured with PL or FBS, revealing a significant upregulation of genes related to stress response and cell proliferation under PL conditions. These findings were validated by RT–qPCR and supported by functional assays demonstrating enhanced cellular resilience to oxidative and genotoxic stress, reduced doxorubicin-induced senescence, and improved antiapoptotic properties. In a murine wound model, PL-treated wounds showed accelerated healing, characterized by thicker dermis-like tissue formation and increased angiogenesis. Immunohistochemical analysis further revealed elevated expression of chk1, a DNA damage response kinase encoded by CHEK1, which plays a central role in maintaining genomic integrity during stress-induced repair. Collectively, these results highlight PL not only as a viable substitute for FBS in hASC expansion but also as a bioactive supplement that enhances regenerative efficacy by promoting proliferation, stress resistance, and antiaging functions.https://www.mdpi.com/2073-4409/14/15/1154human adipose-derived stem cellsplatelet lysatecell proliferationstress resistanceantiaging effectsRNA sequencing |
| spellingShingle | Sakurako Kunieda Michika Fukui Atsuyuki Kuro Toshihito Mitsui Huan Li Zhongxin Sun Takayuki Ueda Shigeru Taketani Koichiro Higasa Natsuko Kakudo Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing Cells human adipose-derived stem cells platelet lysate cell proliferation stress resistance antiaging effects RNA sequencing |
| title | Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing |
| title_full | Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing |
| title_fullStr | Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing |
| title_full_unstemmed | Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing |
| title_short | Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing |
| title_sort | molecular insights into the superiority of platelet lysate over fbs for hasc expansion and wound healing |
| topic | human adipose-derived stem cells platelet lysate cell proliferation stress resistance antiaging effects RNA sequencing |
| url | https://www.mdpi.com/2073-4409/14/15/1154 |
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