“Cassia auriculata-derived silver nanoparticles as a novel male contraceptive agent”

Introduction: The development of male contraception remains a crucial area of research in reproductive health. Nanotechnology offers innovative solutions, with plant-derived nanoparticles emerging as potential antifertility agents. This study explores the antifertility effects of Cassia auriculata-d...

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Main Authors: Prity Yadav, Neha Bharti, Phulwanti Kumari Sharma, Pratap Chand Mali
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Pharmacological Research - Modern Chinese Medicine
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667142525000557
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author Prity Yadav
Neha Bharti
Phulwanti Kumari Sharma
Pratap Chand Mali
author_facet Prity Yadav
Neha Bharti
Phulwanti Kumari Sharma
Pratap Chand Mali
author_sort Prity Yadav
collection DOAJ
description Introduction: The development of male contraception remains a crucial area of research in reproductive health. Nanotechnology offers innovative solutions, with plant-derived nanoparticles emerging as potential antifertility agents. This study explores the antifertility effects of Cassia auriculata-derived silver nanoparticles (AgNPs) as a novel approach to male contraception. Cassia auriculata, a medicinal plant with known therapeutic properties, plants of the genus Cassia are used mainly in traditional Chinese medicine (TCM) to control type 2 diabetes, skin and wound healing, as a mild laxative, and for urinary tract health, with active constituents including 1-Tetradecanol, Anthraquinone, Emodin, Quercetin-3-glucoside, β-sitosterol, Rutin, β-sitosterol-β-D-glucoside, was utilized for the green synthesis of AgNPs, ensuring eco-friendliness and biocompatibility. Methods: The synthesized nanoparticles were characterized using UV-Vis spectroscopy, XRD (X-Ray diffraction), FTIR (Fourier Transform Infra-Red), DLS (Dynamic Light Scattering), Zeta Potential, and SEM (Scanning Electron Microscopy) to confirm their physicochemical properties. Results: In vivo studies on male albino rats revealed significant reductions in sperm density and motility, alongside alterations in testicular and epididymal histology, indicating impaired spermatogenesis. Hormonal analysis showed a decline in testosterone levels, further supporting the antifertility potential of these nanoparticles. Importantly, toxicity assessments confirmed minimal systemic side effects, highlighting their safety profile. Discussion: The findings suggest that Cassia auriculata-derived AgNPs could be a promising non-hormonal male contraceptive with reversible effects. This research underscores the potential of nanotechnology in reproductive health and paves the way for further clinical investigations into plant-based nanoparticle contraceptives.
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spelling doaj-art-6e54d77a8ed94911ab772fb0ac4cadc62025-08-20T03:20:58ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252025-06-011510062610.1016/j.prmcm.2025.100626“Cassia auriculata-derived silver nanoparticles as a novel male contraceptive agent”Prity Yadav0Neha Bharti1Phulwanti Kumari Sharma2Pratap Chand Mali3Reproductive Biomedicine and Natural Products Lab, Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Jaipur 302004, IndiaReproductive Biomedicine and Natural Products Lab, Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Jaipur 302004, IndiaDrug Discovery and Development Unit, National Institute of Ayurveda Jorawar Singh Gate, Amer Road, Jaipur, 302002, IndiaReproductive Biomedicine and Natural Products Lab, Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Jaipur 302004, India; Corresponding author.Introduction: The development of male contraception remains a crucial area of research in reproductive health. Nanotechnology offers innovative solutions, with plant-derived nanoparticles emerging as potential antifertility agents. This study explores the antifertility effects of Cassia auriculata-derived silver nanoparticles (AgNPs) as a novel approach to male contraception. Cassia auriculata, a medicinal plant with known therapeutic properties, plants of the genus Cassia are used mainly in traditional Chinese medicine (TCM) to control type 2 diabetes, skin and wound healing, as a mild laxative, and for urinary tract health, with active constituents including 1-Tetradecanol, Anthraquinone, Emodin, Quercetin-3-glucoside, β-sitosterol, Rutin, β-sitosterol-β-D-glucoside, was utilized for the green synthesis of AgNPs, ensuring eco-friendliness and biocompatibility. Methods: The synthesized nanoparticles were characterized using UV-Vis spectroscopy, XRD (X-Ray diffraction), FTIR (Fourier Transform Infra-Red), DLS (Dynamic Light Scattering), Zeta Potential, and SEM (Scanning Electron Microscopy) to confirm their physicochemical properties. Results: In vivo studies on male albino rats revealed significant reductions in sperm density and motility, alongside alterations in testicular and epididymal histology, indicating impaired spermatogenesis. Hormonal analysis showed a decline in testosterone levels, further supporting the antifertility potential of these nanoparticles. Importantly, toxicity assessments confirmed minimal systemic side effects, highlighting their safety profile. Discussion: The findings suggest that Cassia auriculata-derived AgNPs could be a promising non-hormonal male contraceptive with reversible effects. This research underscores the potential of nanotechnology in reproductive health and paves the way for further clinical investigations into plant-based nanoparticle contraceptives.http://www.sciencedirect.com/science/article/pii/S2667142525000557Male contraceptionCassia auriculataSilver nanoparticlesAntifertilityNanotechnologySpermatogenesis
spellingShingle Prity Yadav
Neha Bharti
Phulwanti Kumari Sharma
Pratap Chand Mali
“Cassia auriculata-derived silver nanoparticles as a novel male contraceptive agent”
Pharmacological Research - Modern Chinese Medicine
Male contraception
Cassia auriculata
Silver nanoparticles
Antifertility
Nanotechnology
Spermatogenesis
title “Cassia auriculata-derived silver nanoparticles as a novel male contraceptive agent”
title_full “Cassia auriculata-derived silver nanoparticles as a novel male contraceptive agent”
title_fullStr “Cassia auriculata-derived silver nanoparticles as a novel male contraceptive agent”
title_full_unstemmed “Cassia auriculata-derived silver nanoparticles as a novel male contraceptive agent”
title_short “Cassia auriculata-derived silver nanoparticles as a novel male contraceptive agent”
title_sort cassia auriculata derived silver nanoparticles as a novel male contraceptive agent
topic Male contraception
Cassia auriculata
Silver nanoparticles
Antifertility
Nanotechnology
Spermatogenesis
url http://www.sciencedirect.com/science/article/pii/S2667142525000557
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