Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis
Abstract Background Ophiocordyceps sinensis (O. sinensis) is the dominant bacterium in the asexual stage of Chinese cordyceps, and its growth usually suffers from water stress. Thus, simulating its ecological growth environment is crucial for artificial cultivation. This study aimed to reveal the me...
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2024-10-01
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| Online Access: | https://doi.org/10.1186/s12864-024-10785-2 |
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| author | Li He ChuanYong Li ZhaoHe Chen YanLi Huo Bo Zhou Fang Xie |
| author_facet | Li He ChuanYong Li ZhaoHe Chen YanLi Huo Bo Zhou Fang Xie |
| author_sort | Li He |
| collection | DOAJ |
| description | Abstract Background Ophiocordyceps sinensis (O. sinensis) is the dominant bacterium in the asexual stage of Chinese cordyceps, and its growth usually suffers from water stress. Thus, simulating its ecological growth environment is crucial for artificial cultivation. This study aimed to reveal the mechanism underlying the water stress tolerance of Ophiocordyceps sinensis (O. sinensis) by combining metabolomic and transcriptome analyses to identify crucial pathways related to differentially expressed genes (DEGs) and metabolites (DEMs) involved in the response to water stress. Results Gene coexpression analysis revealed that many genes related to ‘betalain biosynthesis’, ‘tyrosine metabolism’, ‘linoleic acid metabolism’, ‘fructose and mannose metabolism’, and ‘starch and sucrose metabolism’ were highly upregulated after 20d-water stress. Metabolomic analysis revealed that many metabolites regulated by these genes in these metabolic pathways were markedly decreased. On the one hand, we surmised that carbohydrate metabolism and the β-oxidation pathway worked cooperatively to generate enough acyl-CoA and then entered the TCA cycle to provide energy when exposed to water stress. On the other hand, the betalain biosynthesis and tyrosine metabolism pathway might play crucial roles in response to water stress in O. sinensis by enhancing cell osmotic potential and producing osmoregulatory substances (betaine) and antioxidant pigments (eumelanin). Conclusions Overall, our findings provide important information for further exploration of the mechanism underlying the water stress tolerance of O. sinensis for the industrialization of artificial cultivation of Chinese cordyceps. |
| format | Article |
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| institution | OA Journals |
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| language | English |
| publishDate | 2024-10-01 |
| publisher | BMC |
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| spelling | doaj-art-6e4cbcef7a6b416db5c35087d0eb3e622025-08-20T02:18:19ZengBMCBMC Genomics1471-21642024-10-0125111510.1186/s12864-024-10785-2Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensisLi He0ChuanYong Li1ZhaoHe Chen2YanLi Huo3Bo Zhou4Fang Xie5School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong UniversitySchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong UniversitySchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong UniversitySchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong UniversitySchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong UniversitySchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong UniversityAbstract Background Ophiocordyceps sinensis (O. sinensis) is the dominant bacterium in the asexual stage of Chinese cordyceps, and its growth usually suffers from water stress. Thus, simulating its ecological growth environment is crucial for artificial cultivation. This study aimed to reveal the mechanism underlying the water stress tolerance of Ophiocordyceps sinensis (O. sinensis) by combining metabolomic and transcriptome analyses to identify crucial pathways related to differentially expressed genes (DEGs) and metabolites (DEMs) involved in the response to water stress. Results Gene coexpression analysis revealed that many genes related to ‘betalain biosynthesis’, ‘tyrosine metabolism’, ‘linoleic acid metabolism’, ‘fructose and mannose metabolism’, and ‘starch and sucrose metabolism’ were highly upregulated after 20d-water stress. Metabolomic analysis revealed that many metabolites regulated by these genes in these metabolic pathways were markedly decreased. On the one hand, we surmised that carbohydrate metabolism and the β-oxidation pathway worked cooperatively to generate enough acyl-CoA and then entered the TCA cycle to provide energy when exposed to water stress. On the other hand, the betalain biosynthesis and tyrosine metabolism pathway might play crucial roles in response to water stress in O. sinensis by enhancing cell osmotic potential and producing osmoregulatory substances (betaine) and antioxidant pigments (eumelanin). Conclusions Overall, our findings provide important information for further exploration of the mechanism underlying the water stress tolerance of O. sinensis for the industrialization of artificial cultivation of Chinese cordyceps.https://doi.org/10.1186/s12864-024-10785-2Ophiocordyceps SinensisWater stressRNA-seqWGCNAMetabolites |
| spellingShingle | Li He ChuanYong Li ZhaoHe Chen YanLi Huo Bo Zhou Fang Xie Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis BMC Genomics Ophiocordyceps Sinensis Water stress RNA-seq WGCNA Metabolites |
| title | Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis |
| title_full | Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis |
| title_fullStr | Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis |
| title_full_unstemmed | Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis |
| title_short | Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis |
| title_sort | combined metabolome and transcriptome analysis reveal the mechanism of water stress in ophiocordyceps sinensis |
| topic | Ophiocordyceps Sinensis Water stress RNA-seq WGCNA Metabolites |
| url | https://doi.org/10.1186/s12864-024-10785-2 |
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