Regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy: an immunohistochemical analysis
Objectives: Radiotherapy is a common treatment for head and neck malignancies; however, it frequently affects salivary glands, leading to xerostomia. This study evaluated the effects of radiotherapy on cytokeratin localization in the parotid gland, examining whether changes indicate recovery or pro...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Medical Journals Sweden
2025-08-01
|
| Series: | Acta Odontologica Scandinavica |
| Subjects: | |
| Online Access: | https://medicaljournalssweden.se/actaodontologica/article/view/44182 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849229380546461696 |
|---|---|
| author | Sherif S. Hassan Ehab T. Azab Alaa W. Alqutub Mashael S. Alqahtani Abrar K. Demyati Abdullah A. Holdar Fatma M. Alkassimi Mahmoud A. Attia Reda A. Nofal |
| author_facet | Sherif S. Hassan Ehab T. Azab Alaa W. Alqutub Mashael S. Alqahtani Abrar K. Demyati Abdullah A. Holdar Fatma M. Alkassimi Mahmoud A. Attia Reda A. Nofal |
| author_sort | Sherif S. Hassan |
| collection | DOAJ |
| description |
Objectives: Radiotherapy is a common treatment for head and neck malignancies; however, it frequently affects salivary glands, leading to xerostomia. This study evaluated the effects of radiotherapy on cytokeratin localization in the parotid gland, examining whether changes indicate recovery or progressive damage over a year.
Methods: The study included eight control rats and 16 irradiated rats exposed to 30 Gy of radiation over 6 days. The experiment was conducted from January 2023 to April 2024. Subgroup IIa rats were sacrificed 1 month after radiation exposure, while subgroup IIb rats were sacrificed after 1 year. The parotid gland was prepared for histological and immunohistochemical analysis of intermediate filaments.
Results: In the control parotid gland, immunohistochemical analysis revealed mild cytokeratin in ductal and serous cells. Subgroup IIa exhibited strong cytokeratin expression in the acini and duct cells, which was significantly different from the control group. One year after radiation, the cytokeratin of subgroup IIb was comparable to that of the control, with no significant difference.
Conclusion: In subgroup IIa, cytokeratin staining was notably stronger in ductal and acinar cells, leading to disrupted distribution that impaired saliva production and transport. In subgroup IIb, the redistribution of cytokeratin exhibited distinct recovery patterns in ductal and acinar cells.
|
| format | Article |
| id | doaj-art-6e4ba3ca95204d48b5f12695fc28b548 |
| institution | Kabale University |
| issn | 0001-6357 1502-3850 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Medical Journals Sweden |
| record_format | Article |
| series | Acta Odontologica Scandinavica |
| spelling | doaj-art-6e4ba3ca95204d48b5f12695fc28b5482025-08-21T14:58:18ZengMedical Journals SwedenActa Odontologica Scandinavica0001-63571502-38502025-08-018410.2340/aos.v84.44182Regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy: an immunohistochemical analysisSherif S. Hassan0https://orcid.org/0000-0002-3203-4438Ehab T. Azab1https://orcid.org/0000-0003-3266-1463Alaa W. Alqutub2https://orcid.org/0000-0002-9278-1654Mashael S. Alqahtani3https://orcid.org/0000-0001-9731-6578Abrar K. Demyati4https://orcid.org/0000-0001-7510-8997Abdullah A. Holdar5https://orcid.org/0009-0000-9662-2813Fatma M. Alkassimi6https://orcid.org/0009-0004-6140-4997Mahmoud A. Attia7https://orcid.org/0009-0000-1629-6860Reda A. Nofal8https://orcid.org/0000-0002-5946-0488Department of dental and medical sciences, Faculty of Dentistry, Mutah University, JordanDepartment of dental and medical sciences, Faculty of Dentistry, Mutah University, JordanDepartment of Basic and Clinical Oral Sciences, College of Dental Medicine, Umm Al-Qura University, Makkah, Saudi ArabiDepartment of Basic and Clinical Oral Sciences, College of Dental Medicine, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Oral and Maxillofacial Surgery, College of Dental Medicine, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Basic and Clinical Oral Sciences, College of Dental Medicine, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Basic and Clinical Oral Sciences, College of Dental Medicine, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Oral and Dental Health, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Saudi ArabiaDepartment of Oral and Maxillofacial Surgery, College of Dental Medicine, Umm Al-Qura University, Makkah, Saudi Arabia Objectives: Radiotherapy is a common treatment for head and neck malignancies; however, it frequently affects salivary glands, leading to xerostomia. This study evaluated the effects of radiotherapy on cytokeratin localization in the parotid gland, examining whether changes indicate recovery or progressive damage over a year. Methods: The study included eight control rats and 16 irradiated rats exposed to 30 Gy of radiation over 6 days. The experiment was conducted from January 2023 to April 2024. Subgroup IIa rats were sacrificed 1 month after radiation exposure, while subgroup IIb rats were sacrificed after 1 year. The parotid gland was prepared for histological and immunohistochemical analysis of intermediate filaments. Results: In the control parotid gland, immunohistochemical analysis revealed mild cytokeratin in ductal and serous cells. Subgroup IIa exhibited strong cytokeratin expression in the acini and duct cells, which was significantly different from the control group. One year after radiation, the cytokeratin of subgroup IIb was comparable to that of the control, with no significant difference. Conclusion: In subgroup IIa, cytokeratin staining was notably stronger in ductal and acinar cells, leading to disrupted distribution that impaired saliva production and transport. In subgroup IIb, the redistribution of cytokeratin exhibited distinct recovery patterns in ductal and acinar cells. https://medicaljournalssweden.se/actaodontologica/article/view/44182Regenerationparotid glandcytokeratinradiotherapy |
| spellingShingle | Sherif S. Hassan Ehab T. Azab Alaa W. Alqutub Mashael S. Alqahtani Abrar K. Demyati Abdullah A. Holdar Fatma M. Alkassimi Mahmoud A. Attia Reda A. Nofal Regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy: an immunohistochemical analysis Acta Odontologica Scandinavica Regeneration parotid gland cytokeratin radiotherapy |
| title | Regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy: an immunohistochemical analysis |
| title_full | Regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy: an immunohistochemical analysis |
| title_fullStr | Regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy: an immunohistochemical analysis |
| title_full_unstemmed | Regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy: an immunohistochemical analysis |
| title_short | Regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy: an immunohistochemical analysis |
| title_sort | regenerative potential of the intermediate filaments of albino rat parotid glands subjected to fractionated radiotherapy an immunohistochemical analysis |
| topic | Regeneration parotid gland cytokeratin radiotherapy |
| url | https://medicaljournalssweden.se/actaodontologica/article/view/44182 |
| work_keys_str_mv | AT sherifshassan regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis AT ehabtazab regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis AT alaawalqutub regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis AT mashaelsalqahtani regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis AT abrarkdemyati regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis AT abdullahaholdar regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis AT fatmamalkassimi regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis AT mahmoudaattia regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis AT redaanofal regenerativepotentialoftheintermediatefilamentsofalbinoratparotidglandssubjectedtofractionatedradiotherapyanimmunohistochemicalanalysis |