Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus <i>Penicillium antarcticum</i> KMM 4668
<b>Background:</b> During the cultivation of the marine fungus KMM 4668 on solid agar medium, the morphological instability of the strain was observed. As a result of the selection work, five intra-strain variants, named KMM 4711, KMM 4712, KMM 4713, KMM 4714, and KMM 4715, were obtained...
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| author | Gleb V. Borkunov Natalya N. Kirichuk Viktoria E. Chausova Roman S. Popov Olesya I. Zhuravleva Ekaterina A. Chingizova Ekaterina A. Yurchenko Marina P. Isaeva Anton N. Yurchenko |
| author_facet | Gleb V. Borkunov Natalya N. Kirichuk Viktoria E. Chausova Roman S. Popov Olesya I. Zhuravleva Ekaterina A. Chingizova Ekaterina A. Yurchenko Marina P. Isaeva Anton N. Yurchenko |
| author_sort | Gleb V. Borkunov |
| collection | DOAJ |
| description | <b>Background:</b> During the cultivation of the marine fungus KMM 4668 on solid agar medium, the morphological instability of the strain was observed. As a result of the selection work, five intra-strain variants, named KMM 4711, KMM 4712, KMM 4713, KMM 4714, and KMM 4715, were obtained. <b>Methods:</b> The main objectives of this work were to compare the parent strain and its intra-strain variants using multi-locus phylogenetic analysis and to study the UPLC MS metabolite profiles and cytotoxic activities of their extracts. <b>Results:</b> A study of the original strain, KMM 4668, and its intra-strain variants using multi-locus phylogenetic analysis showed that they are sequence identical and belong to <i>Penicillium antarcticum</i>. UPLC MS metabolite profiling of fungal extracts revealed 20 compounds, including cladosporin-related polyketides, carotane sesquiterpenoids, andrastine meroterpenoids, and alkaloids. It was shown that the intra-strain variants KMM 4713 and KMM 4714 differed most strongly from the others in the increased production of cladosporin-related compounds, carotanoids, and the alkaloid chrysogin. In addition, the influence of fungal extracts on the viability of four mammalian cell lines was investigated. <b>Conclusions:</b> It has been shown that the intra-strain variants of <i>P. antarcticum</i> KMM 4668 may be promising sources of bioactive secondary metabolites. |
| format | Article |
| id | doaj-art-6e40e0f1277a4cbbb47b12870c064a69 |
| institution | DOAJ |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Metabolites |
| spelling | doaj-art-6e40e0f1277a4cbbb47b12870c064a692025-08-20T02:44:32ZengMDPI AGMetabolites2218-19892025-01-011527710.3390/metabo15020077Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus <i>Penicillium antarcticum</i> KMM 4668Gleb V. Borkunov0Natalya N. Kirichuk1Viktoria E. Chausova2Roman S. Popov3Olesya I. Zhuravleva4Ekaterina A. Chingizova5Ekaterina A. Yurchenko6Marina P. Isaeva7Anton N. Yurchenko8G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, Russia<b>Background:</b> During the cultivation of the marine fungus KMM 4668 on solid agar medium, the morphological instability of the strain was observed. As a result of the selection work, five intra-strain variants, named KMM 4711, KMM 4712, KMM 4713, KMM 4714, and KMM 4715, were obtained. <b>Methods:</b> The main objectives of this work were to compare the parent strain and its intra-strain variants using multi-locus phylogenetic analysis and to study the UPLC MS metabolite profiles and cytotoxic activities of their extracts. <b>Results:</b> A study of the original strain, KMM 4668, and its intra-strain variants using multi-locus phylogenetic analysis showed that they are sequence identical and belong to <i>Penicillium antarcticum</i>. UPLC MS metabolite profiling of fungal extracts revealed 20 compounds, including cladosporin-related polyketides, carotane sesquiterpenoids, andrastine meroterpenoids, and alkaloids. It was shown that the intra-strain variants KMM 4713 and KMM 4714 differed most strongly from the others in the increased production of cladosporin-related compounds, carotanoids, and the alkaloid chrysogin. In addition, the influence of fungal extracts on the viability of four mammalian cell lines was investigated. <b>Conclusions:</b> It has been shown that the intra-strain variants of <i>P. antarcticum</i> KMM 4668 may be promising sources of bioactive secondary metabolites.https://www.mdpi.com/2218-1989/15/2/77<i>Penicillium</i>intra-strain phenotypic diversitymulti-locus phylogenetic analysisUHPLC-MScytotoxic activity |
| spellingShingle | Gleb V. Borkunov Natalya N. Kirichuk Viktoria E. Chausova Roman S. Popov Olesya I. Zhuravleva Ekaterina A. Chingizova Ekaterina A. Yurchenko Marina P. Isaeva Anton N. Yurchenko Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus <i>Penicillium antarcticum</i> KMM 4668 Metabolites <i>Penicillium</i> intra-strain phenotypic diversity multi-locus phylogenetic analysis UHPLC-MS cytotoxic activity |
| title | Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus <i>Penicillium antarcticum</i> KMM 4668 |
| title_full | Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus <i>Penicillium antarcticum</i> KMM 4668 |
| title_fullStr | Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus <i>Penicillium antarcticum</i> KMM 4668 |
| title_full_unstemmed | Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus <i>Penicillium antarcticum</i> KMM 4668 |
| title_short | Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus <i>Penicillium antarcticum</i> KMM 4668 |
| title_sort | differences in metabolite profiles and bioactivities of intra strain variants of marine fungus i penicillium antarcticum i kmm 4668 |
| topic | <i>Penicillium</i> intra-strain phenotypic diversity multi-locus phylogenetic analysis UHPLC-MS cytotoxic activity |
| url | https://www.mdpi.com/2218-1989/15/2/77 |
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