Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps
Large prefabricated flaps often suffer from necrosis or poor healing due to a lack of new blood vessels and related factors that promote angiogenesis. The innovative use of adipose-derived stem cell exosomes (ADSC-Exo) resolves the problem of vascularization of prefabricated flaps. We analyzed the d...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/5293850 |
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| _version_ | 1832553264241967104 |
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| author | Jiachao Xiong Zhixiao Liu Minliang Wu Mengyan Sun Yu Xia Yuchong Wang |
| author_facet | Jiachao Xiong Zhixiao Liu Minliang Wu Mengyan Sun Yu Xia Yuchong Wang |
| author_sort | Jiachao Xiong |
| collection | DOAJ |
| description | Large prefabricated flaps often suffer from necrosis or poor healing due to a lack of new blood vessels and related factors that promote angiogenesis. The innovative use of adipose-derived stem cell exosomes (ADSC-Exo) resolves the problem of vascularization of prefabricated flaps. We analyzed the differential microRNA (miRNA) expression in ADSC-Exo using next-generation sequencing (NGS) technology to explore their potential mechanisms in promoting vascularization. We observed that ADSC-Exo could significantly promote the vascularization of artificial dermis prefabricated flaps compared with human foreskin fibroblast exosomes. NGS indicated that there were some differentially expressed miRNAs in both exosomes. Bioinformatics analysis suggested that significantly upregulated hsa-miR-760 and significantly downregulated hsa-miR-423-3p in ADSC-Exo could regulate the expression of the ITGA5 and HDAC5 genes, respectively, to promote the vascularization of skin flaps. In summary, ADSC-Exo can promote skin-flap vascularization, and thereby resolve the problem of insufficient neovascularization of artificial dermis prefabricated flaps, thus expanding the application of prefabricated skin-flap transplantation. |
| format | Article |
| id | doaj-art-6e24dce4324a4ec0be6394ba64bef0bc |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6e24dce4324a4ec0be6394ba64bef0bc2025-02-03T05:54:26ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/52938505293850Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated FlapsJiachao Xiong0Zhixiao Liu1Minliang Wu2Mengyan Sun3Yu Xia4Yuchong Wang5Department of Plastic Surgery, Changhai Hospital, Naval Military Medical University, Shanghai 200433, ChinaDepartment of Histology and Embryology, Naval Military Medical University, Shanghai 200433, ChinaDepartment of Plastic Surgery, Changhai Hospital, Naval Military Medical University, Shanghai 200433, ChinaDepartment of Plastic Surgery, Changhai Hospital, Naval Military Medical University, Shanghai 200433, ChinaDepartment of Plastic Surgery, Changhai Hospital, Naval Military Medical University, Shanghai 200433, ChinaDepartment of Plastic Surgery, Changhai Hospital, Naval Military Medical University, Shanghai 200433, ChinaLarge prefabricated flaps often suffer from necrosis or poor healing due to a lack of new blood vessels and related factors that promote angiogenesis. The innovative use of adipose-derived stem cell exosomes (ADSC-Exo) resolves the problem of vascularization of prefabricated flaps. We analyzed the differential microRNA (miRNA) expression in ADSC-Exo using next-generation sequencing (NGS) technology to explore their potential mechanisms in promoting vascularization. We observed that ADSC-Exo could significantly promote the vascularization of artificial dermis prefabricated flaps compared with human foreskin fibroblast exosomes. NGS indicated that there were some differentially expressed miRNAs in both exosomes. Bioinformatics analysis suggested that significantly upregulated hsa-miR-760 and significantly downregulated hsa-miR-423-3p in ADSC-Exo could regulate the expression of the ITGA5 and HDAC5 genes, respectively, to promote the vascularization of skin flaps. In summary, ADSC-Exo can promote skin-flap vascularization, and thereby resolve the problem of insufficient neovascularization of artificial dermis prefabricated flaps, thus expanding the application of prefabricated skin-flap transplantation.http://dx.doi.org/10.1155/2020/5293850 |
| spellingShingle | Jiachao Xiong Zhixiao Liu Minliang Wu Mengyan Sun Yu Xia Yuchong Wang Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps Stem Cells International |
| title | Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps |
| title_full | Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps |
| title_fullStr | Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps |
| title_full_unstemmed | Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps |
| title_short | Comparison of Proangiogenic Effects of Adipose-Derived Stem Cells and Foreskin Fibroblast Exosomes on Artificial Dermis Prefabricated Flaps |
| title_sort | comparison of proangiogenic effects of adipose derived stem cells and foreskin fibroblast exosomes on artificial dermis prefabricated flaps |
| url | http://dx.doi.org/10.1155/2020/5293850 |
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