S-Gal™: An Autoclavable Dye for Color Selection of Cloned DNA Inserts
Blue/white selection is the standard method for detecting a cloned DNA fragment. In the absence of an insert, uninterrupted expression of the vector-encodeda-complement of β-galactosidase (β-gal), results in the hydrolysis of X-gal (5-bromo-4-chloro-3-indolyl β-d-galactoside) and the subsequent blue...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-05-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01305pf01 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|