Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistance
Abstract Colistin, also known as polymyxin E, is extensively used in veterinary and human medicine as a last line of defense against Gram-negative bacteria. Therefore, it is essential to monitor the development of resistance to this antibiotic to combat infections caused by multidrug-resistant bacte...
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| Format: | Article |
| Language: | English |
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Springer
2025-06-01
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| Series: | Discover Applied Sciences |
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| Online Access: | https://doi.org/10.1007/s42452-025-06671-5 |
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| author | Md. Mukter Hossain Riazul Islam Riad Md. Mashfiq Hossain Khadem Ruhena Begum Md. Shoriful Alam Sumon Ho-Seong Cho Md. Mahfujur Rahman Md Bashir Uddin |
| author_facet | Md. Mukter Hossain Riazul Islam Riad Md. Mashfiq Hossain Khadem Ruhena Begum Md. Shoriful Alam Sumon Ho-Seong Cho Md. Mahfujur Rahman Md Bashir Uddin |
| author_sort | Md. Mukter Hossain |
| collection | DOAJ |
| description | Abstract Colistin, also known as polymyxin E, is extensively used in veterinary and human medicine as a last line of defense against Gram-negative bacteria. Therefore, it is essential to monitor the development of resistance to this antibiotic to combat infections caused by multidrug-resistant bacteria. This study focuses on investigating the presence of the colistin resistance gene mcr-1 in Salmonella isolates. A total of 24 Salmonella spp. were identified and analyzed from 200 cattle fecal samples. Phenotypic disc diffusion and a MIC assay using various antimicrobial agents were performed and assessed using the VITEK2 compact system. The isolates were further examined through PCR targeting the invA and mcr-1 genes. Among the 24 isolates, two exhibited phenotypic resistance to colistin, while one isolate harbored the mcr-1 gene. Notably, 14 (58.33%) of the 24 Salmonella isolates showed multidrug resistance. Furthermore, in-silico functional studies were conducted to explore the genetic relationships and phenotypic correlations among these colistin-resistant isolates. Sequencing and phylogenetic analysis revealed a significant evolutionary association between the catalytic region of Neisseria meningitidis EptA and MCR proteins. Structural modeling indicated that MCR-1 has five essential transmembrane regions for its function in colistin resistance. Molecular docking interaction studies demonstrated distinct binding mechanisms between MCR-1 and EptA proteins. This study highlights the occurrence of mcr-1 carrying Salmonella in cattle from Bangladesh, underscoring the urgent need for robust AMR surveillance and stringent antibiotic use policies in agriculture to mitigate the spread of multidrug-resistant pathogens. |
| format | Article |
| id | doaj-art-6e177e6e40514efc86bc207a3b838371 |
| institution | Kabale University |
| issn | 3004-9261 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Applied Sciences |
| spelling | doaj-art-6e177e6e40514efc86bc207a3b8383712025-08-20T03:45:32ZengSpringerDiscover Applied Sciences3004-92612025-06-017611410.1007/s42452-025-06671-5Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistanceMd. Mukter Hossain0Riazul Islam Riad1Md. Mashfiq Hossain Khadem2Ruhena Begum3Md. Shoriful Alam Sumon4Ho-Seong Cho5Md. Mahfujur Rahman6Md Bashir Uddin7Department of Medicine, Sylhet Agricultural UniversityDepartment of Medicine, Sylhet Agricultural UniversityDepartment of Epidemiology and Public Health, Sylhet Agricultural UniversityDepartment of Medicine, Sylhet Agricultural UniversityDepartment of Medicine, Sylhet Agricultural UniversityCollege of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National UniversityDepartment of Medicine, Sylhet Agricultural UniversityDepartment of Medicine, Sylhet Agricultural UniversityAbstract Colistin, also known as polymyxin E, is extensively used in veterinary and human medicine as a last line of defense against Gram-negative bacteria. Therefore, it is essential to monitor the development of resistance to this antibiotic to combat infections caused by multidrug-resistant bacteria. This study focuses on investigating the presence of the colistin resistance gene mcr-1 in Salmonella isolates. A total of 24 Salmonella spp. were identified and analyzed from 200 cattle fecal samples. Phenotypic disc diffusion and a MIC assay using various antimicrobial agents were performed and assessed using the VITEK2 compact system. The isolates were further examined through PCR targeting the invA and mcr-1 genes. Among the 24 isolates, two exhibited phenotypic resistance to colistin, while one isolate harbored the mcr-1 gene. Notably, 14 (58.33%) of the 24 Salmonella isolates showed multidrug resistance. Furthermore, in-silico functional studies were conducted to explore the genetic relationships and phenotypic correlations among these colistin-resistant isolates. Sequencing and phylogenetic analysis revealed a significant evolutionary association between the catalytic region of Neisseria meningitidis EptA and MCR proteins. Structural modeling indicated that MCR-1 has five essential transmembrane regions for its function in colistin resistance. Molecular docking interaction studies demonstrated distinct binding mechanisms between MCR-1 and EptA proteins. This study highlights the occurrence of mcr-1 carrying Salmonella in cattle from Bangladesh, underscoring the urgent need for robust AMR surveillance and stringent antibiotic use policies in agriculture to mitigate the spread of multidrug-resistant pathogens.https://doi.org/10.1007/s42452-025-06671-5mcr-1SalmonellaCattleMDRBangladesh |
| spellingShingle | Md. Mukter Hossain Riazul Islam Riad Md. Mashfiq Hossain Khadem Ruhena Begum Md. Shoriful Alam Sumon Ho-Seong Cho Md. Mahfujur Rahman Md Bashir Uddin Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistance Discover Applied Sciences mcr-1 Salmonella Cattle MDR Bangladesh |
| title | Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistance |
| title_full | Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistance |
| title_fullStr | Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistance |
| title_full_unstemmed | Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistance |
| title_short | Structural function of multidrug-resistant Salmonella carrying mcr-1 gene in colistin resistance |
| title_sort | structural function of multidrug resistant salmonella carrying mcr 1 gene in colistin resistance |
| topic | mcr-1 Salmonella Cattle MDR Bangladesh |
| url | https://doi.org/10.1007/s42452-025-06671-5 |
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