A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors
Abstract Traditional mouse models for deep vein thrombosis (DVT), frequently utilized in research focused on cancer-associated thrombosis (CAT), reliably induce thrombus formation by obstructing blood flow (BF) in the inferior vena cava (IVC), which does not occur in humans. Therefore, to develop a...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84443-8 |
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| author | Hiroko Tadokoro Yukihide Ota Mari Uomoto Shiro Koizume Shinya Sato Yoshiyasu Nakamura Mitsuyo Yoshihara Yoko Endo-Takahashi Yoichi Negishi Etsuko Miyagi Yohei Miyagi |
| author_facet | Hiroko Tadokoro Yukihide Ota Mari Uomoto Shiro Koizume Shinya Sato Yoshiyasu Nakamura Mitsuyo Yoshihara Yoko Endo-Takahashi Yoichi Negishi Etsuko Miyagi Yohei Miyagi |
| author_sort | Hiroko Tadokoro |
| collection | DOAJ |
| description | Abstract Traditional mouse models for deep vein thrombosis (DVT), frequently utilized in research focused on cancer-associated thrombosis (CAT), reliably induce thrombus formation by obstructing blood flow (BF) in the inferior vena cava (IVC), which does not occur in humans. Therefore, to develop a new DVT model for CAT studies, we implanted an ameroid constrictor (AC), a hygroscopic casein C-shape device, around the IVC and aorta of immunocompromised mice. We evaluated the thrombus 3 and 8 days post-AC implantation and compared it with the traditional model 2 days post-vena cava ligation. The size of each thrombus was measured, and the composition was assessed using histological staining; BF through the IVC was confirmed using ultrasound imaging. The thrombus size variability in the AC and ligation models was equivalent. Compared with thrombi on day 3 post-AC implantation, those on day 8 showed characteristics of human thrombi in the subacute to chronic stage. The BF in the IVC was maintained even on day 8. In summary, the AC model showed reproducibility with no significant difference in thrombus size variability from the traditional ligation model while maintaining the BF of the IVC. |
| format | Article |
| id | doaj-art-6e0b3951d8984bad92cb382b42d7c272 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-6e0b3951d8984bad92cb382b42d7c2722025-01-12T12:18:34ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-024-84443-8A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictorsHiroko Tadokoro0Yukihide Ota1Mari Uomoto2Shiro Koizume3Shinya Sato4Yoshiyasu Nakamura5Mitsuyo Yoshihara6Yoko Endo-Takahashi7Yoichi Negishi8Etsuko Miyagi9Yohei Miyagi10Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research InstituteMolecular Pathology and Genetics Division, Kanagawa Cancer Center Research InstituteMolecular Pathology and Genetics Division, Kanagawa Cancer Center Research InstituteMolecular Pathology and Genetics Division, Kanagawa Cancer Center Research InstituteMolecular Pathology and Genetics Division, Kanagawa Cancer Center Research InstituteMolecular Pathology and Genetics Division, Kanagawa Cancer Center Research InstituteMorphological Information Analysis Laboratory, Kanagawa Cancer Center Research InstituteDepartment of Drug Delivery and Molecular Biopharmaceutics, Tokyo University of Pharmacy and Life SciencesDepartment of Drug Delivery and Molecular Biopharmaceutics, Tokyo University of Pharmacy and Life SciencesDepartment of Obstetrics and Gynecology, Yokohama City University Graduate School of MedicineMolecular Pathology and Genetics Division, Kanagawa Cancer Center Research InstituteAbstract Traditional mouse models for deep vein thrombosis (DVT), frequently utilized in research focused on cancer-associated thrombosis (CAT), reliably induce thrombus formation by obstructing blood flow (BF) in the inferior vena cava (IVC), which does not occur in humans. Therefore, to develop a new DVT model for CAT studies, we implanted an ameroid constrictor (AC), a hygroscopic casein C-shape device, around the IVC and aorta of immunocompromised mice. We evaluated the thrombus 3 and 8 days post-AC implantation and compared it with the traditional model 2 days post-vena cava ligation. The size of each thrombus was measured, and the composition was assessed using histological staining; BF through the IVC was confirmed using ultrasound imaging. The thrombus size variability in the AC and ligation models was equivalent. Compared with thrombi on day 3 post-AC implantation, those on day 8 showed characteristics of human thrombi in the subacute to chronic stage. The BF in the IVC was maintained even on day 8. In summary, the AC model showed reproducibility with no significant difference in thrombus size variability from the traditional ligation model while maintaining the BF of the IVC.https://doi.org/10.1038/s41598-024-84443-8Animal modelNude miceBlood flowCancer-associated thrombosisVenous thrombosisUltrasound image |
| spellingShingle | Hiroko Tadokoro Yukihide Ota Mari Uomoto Shiro Koizume Shinya Sato Yoshiyasu Nakamura Mitsuyo Yoshihara Yoko Endo-Takahashi Yoichi Negishi Etsuko Miyagi Yohei Miyagi A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors Scientific Reports Animal model Nude mice Blood flow Cancer-associated thrombosis Venous thrombosis Ultrasound image |
| title | A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors |
| title_full | A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors |
| title_fullStr | A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors |
| title_full_unstemmed | A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors |
| title_short | A mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors |
| title_sort | mouse model of deep vein thrombosis by inferior vena cava hypoperfusion using ameroid constrictors |
| topic | Animal model Nude mice Blood flow Cancer-associated thrombosis Venous thrombosis Ultrasound image |
| url | https://doi.org/10.1038/s41598-024-84443-8 |
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