Bioinformatic insights into sugar signaling pathways in sugarcane growth
Abstract The SnRK1, hexokinase, and TORC1 (TOR, LST8, RAPTOR) are three pivotal kinases at the core of sugar level sensing, significantly impacting plant metabolism and development. We retrieved and analyzed protein sequences of these three kinase pathways from seven sugarcane transcriptome and geno...
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Nature Portfolio
2024-10-01
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| Online Access: | https://doi.org/10.1038/s41598-024-75220-8 |
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| author | Lauana Pereira de Oliveira João Pedro de Jesus Pereira Bruno Viana Navarro Marina C. M. Martins Diego Mauricio Riaño-Pachón Marcos Silveira Buckeridge |
| author_facet | Lauana Pereira de Oliveira João Pedro de Jesus Pereira Bruno Viana Navarro Marina C. M. Martins Diego Mauricio Riaño-Pachón Marcos Silveira Buckeridge |
| author_sort | Lauana Pereira de Oliveira |
| collection | DOAJ |
| description | Abstract The SnRK1, hexokinase, and TORC1 (TOR, LST8, RAPTOR) are three pivotal kinases at the core of sugar level sensing, significantly impacting plant metabolism and development. We retrieved and analyzed protein sequences of these three kinase pathways from seven sugarcane transcriptome and genome datasets, identifying protein domains, phylogenetic relationships, sequence ancestry, and in silico expression levels. Additionally, we predicted HXK subcellular localization and assessed its enzymatic activity in sugarcane leaves and culms along development in the field. We retrieved 11 TOR, 23 RAPTOR, 55 LST8, 95 SnRK1α, 98 HXK, and 14 HXK-like putative full-length sequences containing all the conserved domains. Most of these transcripts seem to share a common origin with the three ancestral species of sugarcane: Saccharum officinarum, Saccharum spontaneum, and Saccharum barberi. We accessed the expression profile of sequences from one sugarcane transcriptome. We found the highest enzymatic activity of HXK in culms in the first month, which, at this stage, provides carbon (sucrose) and nitrogen (amino acids) for initial plant development. Our approach places novel sugar sensing sequences that work as a guideline for further research into the underlying signaling mechanisms and biotechnology applications in sugarcane. |
| format | Article |
| id | doaj-art-6e057e1271b74f4787bc3d5903ccf22d |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-6e057e1271b74f4787bc3d5903ccf22d2025-08-20T02:11:25ZengNature PortfolioScientific Reports2045-23222024-10-0114111410.1038/s41598-024-75220-8Bioinformatic insights into sugar signaling pathways in sugarcane growthLauana Pereira de Oliveira0João Pedro de Jesus Pereira1Bruno Viana Navarro2Marina C. M. Martins3Diego Mauricio Riaño-Pachón4Marcos Silveira Buckeridge5Laboratório de Fisiologia Ecológica de Plantas, Departamento de Botânica, Instituto de Biociências, Universidade de São PauloLaboratório de Fisiologia Ecológica de Plantas, Departamento de Botânica, Instituto de Biociências, Universidade de São PauloLaboratório de Fisiologia Ecológica de Plantas, Departamento de Botânica, Instituto de Biociências, Universidade de São PauloLaboratório de Fisiologia Ecológica de Plantas, Departamento de Botânica, Instituto de Biociências, Universidade de São PauloLaboratório de Biologia Computacional, Evolutiva e de Sistemas, Centro de Energia Nuclear Na Agricultura, Universidade de São PauloLaboratório de Fisiologia Ecológica de Plantas, Departamento de Botânica, Instituto de Biociências, Universidade de São PauloAbstract The SnRK1, hexokinase, and TORC1 (TOR, LST8, RAPTOR) are three pivotal kinases at the core of sugar level sensing, significantly impacting plant metabolism and development. We retrieved and analyzed protein sequences of these three kinase pathways from seven sugarcane transcriptome and genome datasets, identifying protein domains, phylogenetic relationships, sequence ancestry, and in silico expression levels. Additionally, we predicted HXK subcellular localization and assessed its enzymatic activity in sugarcane leaves and culms along development in the field. We retrieved 11 TOR, 23 RAPTOR, 55 LST8, 95 SnRK1α, 98 HXK, and 14 HXK-like putative full-length sequences containing all the conserved domains. Most of these transcripts seem to share a common origin with the three ancestral species of sugarcane: Saccharum officinarum, Saccharum spontaneum, and Saccharum barberi. We accessed the expression profile of sequences from one sugarcane transcriptome. We found the highest enzymatic activity of HXK in culms in the first month, which, at this stage, provides carbon (sucrose) and nitrogen (amino acids) for initial plant development. Our approach places novel sugar sensing sequences that work as a guideline for further research into the underlying signaling mechanisms and biotechnology applications in sugarcane.https://doi.org/10.1038/s41598-024-75220-8CarbohydratesBioinformaticsSugar signaling kinasesTORSnRK1Hexokinase |
| spellingShingle | Lauana Pereira de Oliveira João Pedro de Jesus Pereira Bruno Viana Navarro Marina C. M. Martins Diego Mauricio Riaño-Pachón Marcos Silveira Buckeridge Bioinformatic insights into sugar signaling pathways in sugarcane growth Scientific Reports Carbohydrates Bioinformatics Sugar signaling kinases TOR SnRK1 Hexokinase |
| title | Bioinformatic insights into sugar signaling pathways in sugarcane growth |
| title_full | Bioinformatic insights into sugar signaling pathways in sugarcane growth |
| title_fullStr | Bioinformatic insights into sugar signaling pathways in sugarcane growth |
| title_full_unstemmed | Bioinformatic insights into sugar signaling pathways in sugarcane growth |
| title_short | Bioinformatic insights into sugar signaling pathways in sugarcane growth |
| title_sort | bioinformatic insights into sugar signaling pathways in sugarcane growth |
| topic | Carbohydrates Bioinformatics Sugar signaling kinases TOR SnRK1 Hexokinase |
| url | https://doi.org/10.1038/s41598-024-75220-8 |
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