Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis
Trichinella spiralis infection in rodents is a well-known model of intestinal inflammation associated with hypermotility. Our aim was to elucidate if Th17 cells were involved in the development of gastrointestinal hypermotility in this experimental model. Intestinal inflammation was observed by hema...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/517052 |
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| _version_ | 1832549098687823872 |
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| author | Yu Fu Wenfeng Wang Jingjing Tong Qi Pan Yanqing Long Wei Qian Xiaohua Hou |
| author_facet | Yu Fu Wenfeng Wang Jingjing Tong Qi Pan Yanqing Long Wei Qian Xiaohua Hou |
| author_sort | Yu Fu |
| collection | DOAJ |
| description | Trichinella spiralis infection in rodents is a well-known model of intestinal inflammation associated with hypermotility. Our aim was to elucidate if Th17 cells were involved in the development of gastrointestinal hypermotility in this experimental model. Intestinal inflammation was observed by hematoxylin-eosin (HE) staining. Jejunal smooth muscle contractility was investigated in response to acetylcholine (Ach). The effects of IL-17 on jejunum smooth muscle contractility were explored. Flow cytometry was used to analyze the proportion of Th17 cells in jejunum. The levels of IL-17, IL-23, and TGF-β1 in jejunum were measured by Western blot. Our results showed that the inflammation in jejunum was severe at 2 weeks postinfection (PI), which was not discernible at 8 weeks PI. Jejunal smooth muscle contractility was increased at 2 weeks PI and kept higher at 12 weeks PI. The proportion of Th17 cells and the expression of IL-17 were upregulated in jejunum at 2 weeks PI and normalized at 8 weeks PI. When jejunual smooth muscle strips were cultured with IL-17, contractions elicited by Ach were enhanced in a concentration-dependent manner. Our data suggest that Th17 cells are increased during acute infection with Trichinella spiralis and IL-17 may contribute to jejunal muscle contractility in mice. |
| format | Article |
| id | doaj-art-6dfeed1921bc41d7bef135ed579394e6 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6dfeed1921bc41d7bef135ed579394e62025-02-03T06:12:11ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/517052517052Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralisYu Fu0Wenfeng Wang1Jingjing Tong2Qi Pan3Yanqing Long4Wei Qian5Xiaohua Hou6Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1277, Wuhan 430022, ChinaDivision of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1277, Wuhan 430022, ChinaDivision of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1277, Wuhan 430022, ChinaDivision of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1277, Wuhan 430022, ChinaDivision of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1277, Wuhan 430022, ChinaDivision of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1277, Wuhan 430022, ChinaDivision of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1277, Wuhan 430022, ChinaTrichinella spiralis infection in rodents is a well-known model of intestinal inflammation associated with hypermotility. Our aim was to elucidate if Th17 cells were involved in the development of gastrointestinal hypermotility in this experimental model. Intestinal inflammation was observed by hematoxylin-eosin (HE) staining. Jejunal smooth muscle contractility was investigated in response to acetylcholine (Ach). The effects of IL-17 on jejunum smooth muscle contractility were explored. Flow cytometry was used to analyze the proportion of Th17 cells in jejunum. The levels of IL-17, IL-23, and TGF-β1 in jejunum were measured by Western blot. Our results showed that the inflammation in jejunum was severe at 2 weeks postinfection (PI), which was not discernible at 8 weeks PI. Jejunal smooth muscle contractility was increased at 2 weeks PI and kept higher at 12 weeks PI. The proportion of Th17 cells and the expression of IL-17 were upregulated in jejunum at 2 weeks PI and normalized at 8 weeks PI. When jejunual smooth muscle strips were cultured with IL-17, contractions elicited by Ach were enhanced in a concentration-dependent manner. Our data suggest that Th17 cells are increased during acute infection with Trichinella spiralis and IL-17 may contribute to jejunal muscle contractility in mice.http://dx.doi.org/10.1155/2009/517052 |
| spellingShingle | Yu Fu Wenfeng Wang Jingjing Tong Qi Pan Yanqing Long Wei Qian Xiaohua Hou Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis Mediators of Inflammation |
| title | Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis |
| title_full | Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis |
| title_fullStr | Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis |
| title_full_unstemmed | Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis |
| title_short | Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis |
| title_sort | th17 a new participant in gut dysfunction in mice infected with trichinella spiralis |
| url | http://dx.doi.org/10.1155/2009/517052 |
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