Effect of DHCR7 on adipocyte differentiation in goats
Cholesterol is regarded as a signaling molecule in regulating the metabolism and function of fat cells, in which 7-Dehydrocholesterol reductase (DHCR7) is a key enzyme that catalyzes the conversion of 7-dehydrocholesterol to cholesterol, however, the exact function of DHCR7 in goat adipocytes remain...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2023.2298399 |
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| author | Zhibin Li Tingting Hu Ruiwen Li Jinlan Li Youli Wang Yanyan Li Yaqiu Lin Yong Wang Xing Jiani |
| author_facet | Zhibin Li Tingting Hu Ruiwen Li Jinlan Li Youli Wang Yanyan Li Yaqiu Lin Yong Wang Xing Jiani |
| author_sort | Zhibin Li |
| collection | DOAJ |
| description | Cholesterol is regarded as a signaling molecule in regulating the metabolism and function of fat cells, in which 7-Dehydrocholesterol reductase (DHCR7) is a key enzyme that catalyzes the conversion of 7-dehydrocholesterol to cholesterol, however, the exact function of DHCR7 in goat adipocytes remains unknown. Here, the effect of DHCR7 on the formation of subcutaneous and intramuscular fat in goats was investigated in vitro, and the result indicated that the mRNA level of DHCR7 showed a gradual downward trend in subcutaneous adipogenesis, but an opposite trend in intramuscular adipogenesis. In the process of subcutaneous preadipocytes differentiation, overexpression of DHCR7 inhibited the expression of adipocytes differentiation marker genes (CEBP/α, CEBP/β, SREBP1 and AP2), lipid metabolism-related genes (AGPAT6, FASN, SCD1 and LPL), and the lipid accumulation. However, in intramuscular preadipocyte differentiation, DHCR7 overexpression showed a promoting effect on adipocyte differentiation marker genes (CEBP/α, CEBP/β, PPARγ and SREBP1) and lipid metabolism-related genes (GPAM, AGPAT6, DGAT1 and SCD1) expression, and on lipid accumulation. In summary, our work demonstrated that DHCR7 played an important role in regulating adipogenic differentiation and lipid metabolism in preadipocytes in goats, which is of great significance for uncovering the underlying molecular mechanism of adipocyte differentiation and improving goat meat quality. |
| format | Article |
| id | doaj-art-6dfd17f94ce74a37b7f64aebfbe9dadb |
| institution | DOAJ |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-6dfd17f94ce74a37b7f64aebfbe9dadb2025-08-20T02:50:22ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2023.2298399Effect of DHCR7 on adipocyte differentiation in goatsZhibin Li0Tingting Hu1Ruiwen Li2Jinlan Li3Youli Wang4Yanyan Li5Yaqiu Lin6Yong Wang7Xing Jiani8College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, ChinaCollege of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, ChinaChengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, ChinaCollege of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, ChinaCollege of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, ChinaCollege of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, ChinaCollege of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, ChinaCollege of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, ChinaCollege of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, ChinaCholesterol is regarded as a signaling molecule in regulating the metabolism and function of fat cells, in which 7-Dehydrocholesterol reductase (DHCR7) is a key enzyme that catalyzes the conversion of 7-dehydrocholesterol to cholesterol, however, the exact function of DHCR7 in goat adipocytes remains unknown. Here, the effect of DHCR7 on the formation of subcutaneous and intramuscular fat in goats was investigated in vitro, and the result indicated that the mRNA level of DHCR7 showed a gradual downward trend in subcutaneous adipogenesis, but an opposite trend in intramuscular adipogenesis. In the process of subcutaneous preadipocytes differentiation, overexpression of DHCR7 inhibited the expression of adipocytes differentiation marker genes (CEBP/α, CEBP/β, SREBP1 and AP2), lipid metabolism-related genes (AGPAT6, FASN, SCD1 and LPL), and the lipid accumulation. However, in intramuscular preadipocyte differentiation, DHCR7 overexpression showed a promoting effect on adipocyte differentiation marker genes (CEBP/α, CEBP/β, PPARγ and SREBP1) and lipid metabolism-related genes (GPAM, AGPAT6, DGAT1 and SCD1) expression, and on lipid accumulation. In summary, our work demonstrated that DHCR7 played an important role in regulating adipogenic differentiation and lipid metabolism in preadipocytes in goats, which is of great significance for uncovering the underlying molecular mechanism of adipocyte differentiation and improving goat meat quality.https://www.tandfonline.com/doi/10.1080/10495398.2023.2298399DHCR7subcutaneous adipocyteintramuscular adipocyteadipocyte differentiationgoat |
| spellingShingle | Zhibin Li Tingting Hu Ruiwen Li Jinlan Li Youli Wang Yanyan Li Yaqiu Lin Yong Wang Xing Jiani Effect of DHCR7 on adipocyte differentiation in goats Animal Biotechnology DHCR7 subcutaneous adipocyte intramuscular adipocyte adipocyte differentiation goat |
| title | Effect of DHCR7 on adipocyte differentiation in goats |
| title_full | Effect of DHCR7 on adipocyte differentiation in goats |
| title_fullStr | Effect of DHCR7 on adipocyte differentiation in goats |
| title_full_unstemmed | Effect of DHCR7 on adipocyte differentiation in goats |
| title_short | Effect of DHCR7 on adipocyte differentiation in goats |
| title_sort | effect of dhcr7 on adipocyte differentiation in goats |
| topic | DHCR7 subcutaneous adipocyte intramuscular adipocyte adipocyte differentiation goat |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2023.2298399 |
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