Recent advances in the biosynthesis of polysaccharide-based antimicrobial glycoconjugate vaccines
Glycoconjugate vaccines are a vital category of effective and safe commercial vaccines that have significantly reduced the global prevalence of drug-resistant bacterial infections. These vaccines are synthesized by covalently linking bacterial polysaccharide antigens to a carrier protein. Given that...
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| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1457908/full |
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| author | Yuhui Wang Yuhui Wang Yuhui Wang Yuhui Wang Haodi Liu Haodi Liu Haodi Liu Haodi Liu Baoying Wang Baoying Wang Baoying Wang Baoying Wang Gülzire Gheyret Jingliang Qin Hanlin Wang Hanlin Wang Hanlin Wang Hanlin Wang Yuhan Di Yuhan Di Yuhan Di Yuhan Di Yanling Wang Juan Wang Haining Tan Haining Tan Haining Tan Haining Tan |
| author_facet | Yuhui Wang Yuhui Wang Yuhui Wang Yuhui Wang Haodi Liu Haodi Liu Haodi Liu Haodi Liu Baoying Wang Baoying Wang Baoying Wang Baoying Wang Gülzire Gheyret Jingliang Qin Hanlin Wang Hanlin Wang Hanlin Wang Hanlin Wang Yuhan Di Yuhan Di Yuhan Di Yuhan Di Yanling Wang Juan Wang Haining Tan Haining Tan Haining Tan Haining Tan |
| author_sort | Yuhui Wang |
| collection | DOAJ |
| description | Glycoconjugate vaccines are a vital category of effective and safe commercial vaccines that have significantly reduced the global prevalence of drug-resistant bacterial infections. These vaccines are synthesized by covalently linking bacterial polysaccharide antigens to a carrier protein. Given that they produce a stronger and longer-lasting immune response than pure polysaccharides that activate only B cells, glycoconjugate vaccines have become one of the most promising vaccine types. However, the chemical synthesis of glycoconjugate vaccines is complex, costly, and labor-intensive. Therefore, the efficient preparation of biosynthetic glycoconjugates using microbial cell factories has emerged as a highly desirable manufacturing alternative. This review focuses on advancements in the recombinant microbial biosynthesis of glycoconjugate vaccines and summarizes various strategies to optimize their production. It is based on three key aspects: the selection of oligosaccharyltransferase (OST), the use of different vaccine carrier proteins, and the enhancement of key concentrations in the uridine diphosphate (UDP)-sugar supply. Finally, the review highlights technical challenges and discusses future directions for the recombinant synthesis of glycoconjugate vaccines. |
| format | Article |
| id | doaj-art-6dfb484053814de7b20de1014efd9957 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-6dfb484053814de7b20de1014efd99572025-01-29T06:45:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14579081457908Recent advances in the biosynthesis of polysaccharide-based antimicrobial glycoconjugate vaccinesYuhui Wang0Yuhui Wang1Yuhui Wang2Yuhui Wang3Haodi Liu4Haodi Liu5Haodi Liu6Haodi Liu7Baoying Wang8Baoying Wang9Baoying Wang10Baoying Wang11Gülzire Gheyret12Jingliang Qin13Hanlin Wang14Hanlin Wang15Hanlin Wang16Hanlin Wang17Yuhan Di18Yuhan Di19Yuhan Di20Yuhan Di21Yanling Wang22Juan Wang23Haining Tan24Haining Tan25Haining Tan26Haining Tan27National Glycoengineering Research Center, Shandong University, Qingdao, ChinaNMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao, ChinaShandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao, ChinaSchool of Pharmaceutical Sciences, Shandong University, Qingdao, ChinaNational Glycoengineering Research Center, Shandong University, Qingdao, ChinaNMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao, ChinaShandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao, ChinaSchool of Pharmaceutical Sciences, Shandong University, Qingdao, ChinaNational Glycoengineering Research Center, Shandong University, Qingdao, ChinaNMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao, ChinaShandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao, ChinaSchool of Pharmaceutical Sciences, Shandong University, Qingdao, ChinaSchool of Life Sciences, Shandong University, Qingdao, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education Nankai University, Tianjin, ChinaNational Glycoengineering Research Center, Shandong University, Qingdao, ChinaNMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao, ChinaShandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao, ChinaSchool of Pharmaceutical Sciences, Shandong University, Qingdao, ChinaNational Glycoengineering Research Center, Shandong University, Qingdao, ChinaNMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao, ChinaShandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao, ChinaSchool of Pharmaceutical Sciences, Shandong University, Qingdao, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education Nankai University, Tianjin, ChinaJinan Maternity and Child Care Hospital, Jinan, ChinaNational Glycoengineering Research Center, Shandong University, Qingdao, ChinaNMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao, ChinaShandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao, ChinaSchool of Pharmaceutical Sciences, Shandong University, Qingdao, ChinaGlycoconjugate vaccines are a vital category of effective and safe commercial vaccines that have significantly reduced the global prevalence of drug-resistant bacterial infections. These vaccines are synthesized by covalently linking bacterial polysaccharide antigens to a carrier protein. Given that they produce a stronger and longer-lasting immune response than pure polysaccharides that activate only B cells, glycoconjugate vaccines have become one of the most promising vaccine types. However, the chemical synthesis of glycoconjugate vaccines is complex, costly, and labor-intensive. Therefore, the efficient preparation of biosynthetic glycoconjugates using microbial cell factories has emerged as a highly desirable manufacturing alternative. This review focuses on advancements in the recombinant microbial biosynthesis of glycoconjugate vaccines and summarizes various strategies to optimize their production. It is based on three key aspects: the selection of oligosaccharyltransferase (OST), the use of different vaccine carrier proteins, and the enhancement of key concentrations in the uridine diphosphate (UDP)-sugar supply. Finally, the review highlights technical challenges and discusses future directions for the recombinant synthesis of glycoconjugate vaccines.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1457908/fullglycoconjugate vaccinebiosynthesisoptimization methodglycosyltransferaseglycoengineering |
| spellingShingle | Yuhui Wang Yuhui Wang Yuhui Wang Yuhui Wang Haodi Liu Haodi Liu Haodi Liu Haodi Liu Baoying Wang Baoying Wang Baoying Wang Baoying Wang Gülzire Gheyret Jingliang Qin Hanlin Wang Hanlin Wang Hanlin Wang Hanlin Wang Yuhan Di Yuhan Di Yuhan Di Yuhan Di Yanling Wang Juan Wang Haining Tan Haining Tan Haining Tan Haining Tan Recent advances in the biosynthesis of polysaccharide-based antimicrobial glycoconjugate vaccines Frontiers in Microbiology glycoconjugate vaccine biosynthesis optimization method glycosyltransferase glycoengineering |
| title | Recent advances in the biosynthesis of polysaccharide-based antimicrobial glycoconjugate vaccines |
| title_full | Recent advances in the biosynthesis of polysaccharide-based antimicrobial glycoconjugate vaccines |
| title_fullStr | Recent advances in the biosynthesis of polysaccharide-based antimicrobial glycoconjugate vaccines |
| title_full_unstemmed | Recent advances in the biosynthesis of polysaccharide-based antimicrobial glycoconjugate vaccines |
| title_short | Recent advances in the biosynthesis of polysaccharide-based antimicrobial glycoconjugate vaccines |
| title_sort | recent advances in the biosynthesis of polysaccharide based antimicrobial glycoconjugate vaccines |
| topic | glycoconjugate vaccine biosynthesis optimization method glycosyltransferase glycoengineering |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1457908/full |
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