Synergistic Response Mechanisms in Rice Seedlings Exposed to Brown Planthopper Infestation and High-Temperature Stress
Recently, rice yield has been severely affected by both brown planthopper (BPH, <i>Nilaparvata lugens</i>) infestation and high-temperature stress. Numerous previous studies have identified genes conferring resistance to BPH and high-temperature tolerance in rice, respectively. However,...
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2025-05-01
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| author | Danyun Cao Yuchen Ping Yiru Lin Jinyan Hu Zimeng Wang Wei Yuan Tongtong Li Linxin Liu Bo Zhang Shijiao Xiong Cong Dang Dawei Xue |
| author_facet | Danyun Cao Yuchen Ping Yiru Lin Jinyan Hu Zimeng Wang Wei Yuan Tongtong Li Linxin Liu Bo Zhang Shijiao Xiong Cong Dang Dawei Xue |
| author_sort | Danyun Cao |
| collection | DOAJ |
| description | Recently, rice yield has been severely affected by both brown planthopper (BPH, <i>Nilaparvata lugens</i>) infestation and high-temperature stress. Numerous previous studies have identified genes conferring resistance to BPH and high-temperature tolerance in rice, respectively. However, it remains unclear how rice synergistically responds to these two stress factors. In the present study, we found that pre-treatment with high temperature can enhance rice seeding resistance to BPH, while BPH feeding did not alter the high-temperature tolerance of rice. This result can be elucidated by the subsequent transcriptome analysis. Differentially expressed genes (DEGs) following high-temperature treatment were enriched in metabolic processes and phenylpropanoid biosynthesis pathways, thereby enhancing rice resistance to BPH. Further weighted gene co-expression network analysis (WGCNA) indicated that genes in the magenta and black modules were predominantly associated with the protein folding and transmembrane transport biological processes. And several candidate genes, including <i>Loc_Os01g02170</i> and <i>Loc_Os01g59870</i>, were identified that may play crucial roles in simultaneously regulating rice resistance to BPH and high-temperature stress. This research will provide new gene resources for cultivating rice with compound traits and provide ideas for the mechanism analysis of rice response to multiple stresses. |
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| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-6df4e7a7aac54f7dbc4de852c25e29922025-08-20T02:23:04ZengMDPI AGPlants2223-77472025-05-011411164410.3390/plants14111644Synergistic Response Mechanisms in Rice Seedlings Exposed to Brown Planthopper Infestation and High-Temperature StressDanyun Cao0Yuchen Ping1Yiru Lin2Jinyan Hu3Zimeng Wang4Wei Yuan5Tongtong Li6Linxin Liu7Bo Zhang8Shijiao Xiong9Cong Dang10Dawei Xue11College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaNational Key Laboratory of Rice Biology and Breeding, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaXianghu Laboratory, Hangzhou 311231, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaRecently, rice yield has been severely affected by both brown planthopper (BPH, <i>Nilaparvata lugens</i>) infestation and high-temperature stress. Numerous previous studies have identified genes conferring resistance to BPH and high-temperature tolerance in rice, respectively. However, it remains unclear how rice synergistically responds to these two stress factors. In the present study, we found that pre-treatment with high temperature can enhance rice seeding resistance to BPH, while BPH feeding did not alter the high-temperature tolerance of rice. This result can be elucidated by the subsequent transcriptome analysis. Differentially expressed genes (DEGs) following high-temperature treatment were enriched in metabolic processes and phenylpropanoid biosynthesis pathways, thereby enhancing rice resistance to BPH. Further weighted gene co-expression network analysis (WGCNA) indicated that genes in the magenta and black modules were predominantly associated with the protein folding and transmembrane transport biological processes. And several candidate genes, including <i>Loc_Os01g02170</i> and <i>Loc_Os01g59870</i>, were identified that may play crucial roles in simultaneously regulating rice resistance to BPH and high-temperature stress. This research will provide new gene resources for cultivating rice with compound traits and provide ideas for the mechanism analysis of rice response to multiple stresses.https://www.mdpi.com/2223-7747/14/11/1644synergistic responsetranscriptomebrown planthopperhigh temperaturestressrice |
| spellingShingle | Danyun Cao Yuchen Ping Yiru Lin Jinyan Hu Zimeng Wang Wei Yuan Tongtong Li Linxin Liu Bo Zhang Shijiao Xiong Cong Dang Dawei Xue Synergistic Response Mechanisms in Rice Seedlings Exposed to Brown Planthopper Infestation and High-Temperature Stress Plants synergistic response transcriptome brown planthopper high temperature stress rice |
| title | Synergistic Response Mechanisms in Rice Seedlings Exposed to Brown Planthopper Infestation and High-Temperature Stress |
| title_full | Synergistic Response Mechanisms in Rice Seedlings Exposed to Brown Planthopper Infestation and High-Temperature Stress |
| title_fullStr | Synergistic Response Mechanisms in Rice Seedlings Exposed to Brown Planthopper Infestation and High-Temperature Stress |
| title_full_unstemmed | Synergistic Response Mechanisms in Rice Seedlings Exposed to Brown Planthopper Infestation and High-Temperature Stress |
| title_short | Synergistic Response Mechanisms in Rice Seedlings Exposed to Brown Planthopper Infestation and High-Temperature Stress |
| title_sort | synergistic response mechanisms in rice seedlings exposed to brown planthopper infestation and high temperature stress |
| topic | synergistic response transcriptome brown planthopper high temperature stress rice |
| url | https://www.mdpi.com/2223-7747/14/11/1644 |
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