Causal effect of gut microbiota metabolic pathways on CSAG1 expression in chondrosarcoma: a mendelian randomization analysis

Abstract Background Changes in gut microbiota metabolism might play an important role in the development of some cancers. However, the causal relationships of gut microbiome-related metabolic pathways in chondrosarcomas and the specific pathways affected remain largely unknown. Methods We used two-s...

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Main Authors: Xiaoyun Liu, Sen Yang, Liyun Liu
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Cancer
Subjects:
Online Access:https://doi.org/10.1186/s12885-025-14281-y
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author Xiaoyun Liu
Sen Yang
Liyun Liu
author_facet Xiaoyun Liu
Sen Yang
Liyun Liu
author_sort Xiaoyun Liu
collection DOAJ
description Abstract Background Changes in gut microbiota metabolism might play an important role in the development of some cancers. However, the causal relationships of gut microbiome-related metabolic pathways in chondrosarcomas and the specific pathways affected remain largely unknown. Methods We used two-sample bidirectional and multivariate Mendelian randomization (MR) to reveal a causal relationship between the gut microbiota metabolic pathway (GMMP) and chondrosarcoma associated gene 1(CSAG1) via the largest available genome-wide association study (GWAS). Results Univariate MR analysis revealed that tetrapyrrole biosynthesis from glutamate, menaquinol 6 biosynthesis, glycogen degradation II, 8-amino-7-oxononanoate biosynthesis, taxadiene biosynthesis, glycolysis and tRNA charging had a significant causal relationship with CSAG1.Multivariate MR analysis suggested that tetrapyrrole biosynthesis, menaquinol 6 biosynthesis, glycogen degradation II, glycolysis and tRNA charging still had a significant causal effect on CSAG1. According to the results of reverse MR analysis, no significant causal effect of CSAG1 on the GMMP was found. Conclusions This study offers further insights into the gut microbiota-mediated mechanism of chondrosarcoma development.
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spelling doaj-art-6df2c0164c87412da8bc1efc4cba6d5e2025-08-20T02:15:12ZengBMCBMC Cancer1471-24072025-05-0125111210.1186/s12885-025-14281-yCausal effect of gut microbiota metabolic pathways on CSAG1 expression in chondrosarcoma: a mendelian randomization analysisXiaoyun Liu0Sen Yang1Liyun Liu2Department of General Medical, The People’s Hospital of Changzhi City, The Third Clinical Hospital of Changzhi Medical UniversityDepartment of Orthopedics, The People’s Hospital of Changzhi City, The Third Clinical Hospital of Changzhi Medical UniversityDepartment of Clinical Medicine, The Peace Hospital of Changzhi City, The First Clinical Hospital of Changzhi Medical UniversityAbstract Background Changes in gut microbiota metabolism might play an important role in the development of some cancers. However, the causal relationships of gut microbiome-related metabolic pathways in chondrosarcomas and the specific pathways affected remain largely unknown. Methods We used two-sample bidirectional and multivariate Mendelian randomization (MR) to reveal a causal relationship between the gut microbiota metabolic pathway (GMMP) and chondrosarcoma associated gene 1(CSAG1) via the largest available genome-wide association study (GWAS). Results Univariate MR analysis revealed that tetrapyrrole biosynthesis from glutamate, menaquinol 6 biosynthesis, glycogen degradation II, 8-amino-7-oxononanoate biosynthesis, taxadiene biosynthesis, glycolysis and tRNA charging had a significant causal relationship with CSAG1.Multivariate MR analysis suggested that tetrapyrrole biosynthesis, menaquinol 6 biosynthesis, glycogen degradation II, glycolysis and tRNA charging still had a significant causal effect on CSAG1. According to the results of reverse MR analysis, no significant causal effect of CSAG1 on the GMMP was found. Conclusions This study offers further insights into the gut microbiota-mediated mechanism of chondrosarcoma development.https://doi.org/10.1186/s12885-025-14281-yMendelian randomizationGut microbiota metabolic pathwayChondrosarcomaChondrosarcoma associated gene 1Causal relationship
spellingShingle Xiaoyun Liu
Sen Yang
Liyun Liu
Causal effect of gut microbiota metabolic pathways on CSAG1 expression in chondrosarcoma: a mendelian randomization analysis
BMC Cancer
Mendelian randomization
Gut microbiota metabolic pathway
Chondrosarcoma
Chondrosarcoma associated gene 1
Causal relationship
title Causal effect of gut microbiota metabolic pathways on CSAG1 expression in chondrosarcoma: a mendelian randomization analysis
title_full Causal effect of gut microbiota metabolic pathways on CSAG1 expression in chondrosarcoma: a mendelian randomization analysis
title_fullStr Causal effect of gut microbiota metabolic pathways on CSAG1 expression in chondrosarcoma: a mendelian randomization analysis
title_full_unstemmed Causal effect of gut microbiota metabolic pathways on CSAG1 expression in chondrosarcoma: a mendelian randomization analysis
title_short Causal effect of gut microbiota metabolic pathways on CSAG1 expression in chondrosarcoma: a mendelian randomization analysis
title_sort causal effect of gut microbiota metabolic pathways on csag1 expression in chondrosarcoma a mendelian randomization analysis
topic Mendelian randomization
Gut microbiota metabolic pathway
Chondrosarcoma
Chondrosarcoma associated gene 1
Causal relationship
url https://doi.org/10.1186/s12885-025-14281-y
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AT senyang causaleffectofgutmicrobiotametabolicpathwaysoncsag1expressioninchondrosarcomaamendelianrandomizationanalysis
AT liyunliu causaleffectofgutmicrobiotametabolicpathwaysoncsag1expressioninchondrosarcomaamendelianrandomizationanalysis