Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice
Abstract Background Polyploid rice has a high yield potential and excellent nutritional quality. The development of polyploid rice remained critically limited for several decades due to low seed setting rate until the successful breeding of polyploid meiosis stability (PMeS) lines. To determine the...
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2025-05-01
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| Online Access: | https://doi.org/10.1186/s12870-025-06672-x |
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| author | Pincang Lv Man Wang Rongjie Qiu Chang Yao Meng Fang Yuandong Xing Xianhua Zhang Yuchi He Detian Cai Zhaojian Song |
| author_facet | Pincang Lv Man Wang Rongjie Qiu Chang Yao Meng Fang Yuandong Xing Xianhua Zhang Yuchi He Detian Cai Zhaojian Song |
| author_sort | Pincang Lv |
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| description | Abstract Background Polyploid rice has a high yield potential and excellent nutritional quality. The development of polyploid rice remained critically limited for several decades due to low seed setting rate until the successful breeding of polyploid meiosis stability (PMeS) lines. To determine the mechanism responsible for meiotic stability and high seed setting rate of PMeS line, agronomic traits, pollen fertility and viability, and meiotic behaviors of PMeS and non-PMeS lines were investigated. Further, comparative transcriptome analysis was performed to identify genes associated with meiotic stability and high seed setting rate in PMeS line. Results The seed setting rate, fertile and viable pollen ratios of PMeS line were significantly higher than those of non-PMeS line. The PMeS line exhibited stable meiosis, and chromosomes mainly paired as bivalents, rarely as univalents and multivalents in prophase I. Few lagging chromosomes were observed in anaphase I. By contrast, the homologous chromosomes pairing was disorganized in the non-PMeS line, with low frequencies of bivalents and high frequencies of univalents and multivalents in prophase I, while more cells with increased lagging chromosomes were detected in anaphase I. Many differentially expressed genes (DEGs) between PMeS and non-PMeS lines were identified through comparative transcriptome analysis. Some meiosis-related genes were specifically investigated from all DEGs. Further, several meiotic genes were identified as candidate genes. Conclusions The study not only demonstrates the morphological, cytological, and molecular differences between the PMeS and non-PMeS lines, but also provides several key genes associated with meiotic stability and high seed setting rate in tetraploid rice. |
| format | Article |
| id | doaj-art-6de8665a13c14f8694063ab227fbbbbc |
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| issn | 1471-2229 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-6de8665a13c14f8694063ab227fbbbbc2025-08-20T01:51:38ZengBMCBMC Plant Biology1471-22292025-05-0125111410.1186/s12870-025-06672-xComparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid ricePincang Lv0Man Wang1Rongjie Qiu2Chang Yao3Meng Fang4Yuandong Xing5Xianhua Zhang6Yuchi He7Detian Cai8Zhaojian Song9State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityAbstract Background Polyploid rice has a high yield potential and excellent nutritional quality. The development of polyploid rice remained critically limited for several decades due to low seed setting rate until the successful breeding of polyploid meiosis stability (PMeS) lines. To determine the mechanism responsible for meiotic stability and high seed setting rate of PMeS line, agronomic traits, pollen fertility and viability, and meiotic behaviors of PMeS and non-PMeS lines were investigated. Further, comparative transcriptome analysis was performed to identify genes associated with meiotic stability and high seed setting rate in PMeS line. Results The seed setting rate, fertile and viable pollen ratios of PMeS line were significantly higher than those of non-PMeS line. The PMeS line exhibited stable meiosis, and chromosomes mainly paired as bivalents, rarely as univalents and multivalents in prophase I. Few lagging chromosomes were observed in anaphase I. By contrast, the homologous chromosomes pairing was disorganized in the non-PMeS line, with low frequencies of bivalents and high frequencies of univalents and multivalents in prophase I, while more cells with increased lagging chromosomes were detected in anaphase I. Many differentially expressed genes (DEGs) between PMeS and non-PMeS lines were identified through comparative transcriptome analysis. Some meiosis-related genes were specifically investigated from all DEGs. Further, several meiotic genes were identified as candidate genes. Conclusions The study not only demonstrates the morphological, cytological, and molecular differences between the PMeS and non-PMeS lines, but also provides several key genes associated with meiotic stability and high seed setting rate in tetraploid rice.https://doi.org/10.1186/s12870-025-06672-xTetraploid riceHigh seed setting rateMeiotic stabilityMeiotic genesTranscriptome analysis |
| spellingShingle | Pincang Lv Man Wang Rongjie Qiu Chang Yao Meng Fang Yuandong Xing Xianhua Zhang Yuchi He Detian Cai Zhaojian Song Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice BMC Plant Biology Tetraploid rice High seed setting rate Meiotic stability Meiotic genes Transcriptome analysis |
| title | Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice |
| title_full | Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice |
| title_fullStr | Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice |
| title_full_unstemmed | Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice |
| title_short | Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice |
| title_sort | comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice |
| topic | Tetraploid rice High seed setting rate Meiotic stability Meiotic genes Transcriptome analysis |
| url | https://doi.org/10.1186/s12870-025-06672-x |
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