LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17
Objective. This study aimed to explore the roles and mechanisms of lncRNA FAM225B and PDIA4 in ovarian cancer. Methods. RT-qPCR and Western blot assays were performed to detect the expression levels of the lncRNAs FAM225B, DDX17, and PDIA4 in the serum of patients with ovarian cancer and cell lines....
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | The Breast Journal |
| Online Access: | http://dx.doi.org/10.1155/2023/3970444 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547228810477568 |
|---|---|
| author | Chanjiao Yao Lingjuan Zeng Qin Liu Xiaoxin Qiu Chunyan Chen |
| author_facet | Chanjiao Yao Lingjuan Zeng Qin Liu Xiaoxin Qiu Chunyan Chen |
| author_sort | Chanjiao Yao |
| collection | DOAJ |
| description | Objective. This study aimed to explore the roles and mechanisms of lncRNA FAM225B and PDIA4 in ovarian cancer. Methods. RT-qPCR and Western blot assays were performed to detect the expression levels of the lncRNAs FAM225B, DDX17, and PDIA4 in the serum of patients with ovarian cancer and cell lines. Cells were transfected with lncRNA FAM225B- and PDIA4-related vectors to determine the malignant phenotypes using functional experiments. The mutual binding of lncRNA FAM225B and DDX17 was verified using RNA pull-down and RIP assays. Results. The expression of lncRNAs FAM225B and PDIA4 was decreased in the serum of patients with ovarian cancer and cell lines. Restoration of lncRNA FAM225B or PDIA4 reduced cell proliferation, migration, and invasion abilities and elevated the apoptosis rate, whereas suppression of lncRNA FAM225B or PDIA4 exhibited an inverse trend. RNA pull-down and RIP assays revealed a direct interaction between lncRNA FAM225B and DDX17. ChIP assay revealed a relationship between DDX17 and the PDIA4 promoter. LncRNA FAM225B and DDX17 positively regulate PDIA4 expression. Downregulation of PDIA4 expression counteracts the suppressive effect of lncRNA FAM225B overexpression in ovarian cancer cells. Conclusion. This research study supports the fact that lncRNA FAM225B in ovarian cancer can upregulate PDIA4 by directly binding to DDX17, inhibiting the activities of ovarian cancer cells. |
| format | Article |
| id | doaj-art-6ddfd6a35e154841aa11de1b8932ee81 |
| institution | Kabale University |
| issn | 1524-4741 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Breast Journal |
| spelling | doaj-art-6ddfd6a35e154841aa11de1b8932ee812025-02-03T06:45:31ZengWileyThe Breast Journal1524-47412023-01-01202310.1155/2023/3970444LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17Chanjiao Yao0Lingjuan Zeng1Qin Liu2Xiaoxin Qiu3Chunyan Chen4No. 2 Obstetrics and Gynecology DepartmentNo. 2 Obstetrics and Gynecology DepartmentNo. 2 Obstetrics and Gynecology DepartmentObstetrics and Gynecology DepartmentNo. 2 Obstetrics and Gynecology DepartmentObjective. This study aimed to explore the roles and mechanisms of lncRNA FAM225B and PDIA4 in ovarian cancer. Methods. RT-qPCR and Western blot assays were performed to detect the expression levels of the lncRNAs FAM225B, DDX17, and PDIA4 in the serum of patients with ovarian cancer and cell lines. Cells were transfected with lncRNA FAM225B- and PDIA4-related vectors to determine the malignant phenotypes using functional experiments. The mutual binding of lncRNA FAM225B and DDX17 was verified using RNA pull-down and RIP assays. Results. The expression of lncRNAs FAM225B and PDIA4 was decreased in the serum of patients with ovarian cancer and cell lines. Restoration of lncRNA FAM225B or PDIA4 reduced cell proliferation, migration, and invasion abilities and elevated the apoptosis rate, whereas suppression of lncRNA FAM225B or PDIA4 exhibited an inverse trend. RNA pull-down and RIP assays revealed a direct interaction between lncRNA FAM225B and DDX17. ChIP assay revealed a relationship between DDX17 and the PDIA4 promoter. LncRNA FAM225B and DDX17 positively regulate PDIA4 expression. Downregulation of PDIA4 expression counteracts the suppressive effect of lncRNA FAM225B overexpression in ovarian cancer cells. Conclusion. This research study supports the fact that lncRNA FAM225B in ovarian cancer can upregulate PDIA4 by directly binding to DDX17, inhibiting the activities of ovarian cancer cells.http://dx.doi.org/10.1155/2023/3970444 |
| spellingShingle | Chanjiao Yao Lingjuan Zeng Qin Liu Xiaoxin Qiu Chunyan Chen LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17 The Breast Journal |
| title | LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17 |
| title_full | LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17 |
| title_fullStr | LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17 |
| title_full_unstemmed | LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17 |
| title_short | LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17 |
| title_sort | lncrna fam225b regulates pdia4 mediated ovarian cancer cell invasion and migration via modulating transcription factor ddx17 |
| url | http://dx.doi.org/10.1155/2023/3970444 |
| work_keys_str_mv | AT chanjiaoyao lncrnafam225bregulatespdia4mediatedovariancancercellinvasionandmigrationviamodulatingtranscriptionfactorddx17 AT lingjuanzeng lncrnafam225bregulatespdia4mediatedovariancancercellinvasionandmigrationviamodulatingtranscriptionfactorddx17 AT qinliu lncrnafam225bregulatespdia4mediatedovariancancercellinvasionandmigrationviamodulatingtranscriptionfactorddx17 AT xiaoxinqiu lncrnafam225bregulatespdia4mediatedovariancancercellinvasionandmigrationviamodulatingtranscriptionfactorddx17 AT chunyanchen lncrnafam225bregulatespdia4mediatedovariancancercellinvasionandmigrationviamodulatingtranscriptionfactorddx17 |