Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based Sensors
Procymidone (PCM) is an effective, low-toxicity fungicide commonly used to control plant diseases in grains, vegetables, and fruits. Its usage has significantly increased in recent years, resulting in higher residues in vegetables. This study developed a sensitive and rapid immunoassay method utiliz...
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MDPI AG
2025-04-01
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| author | Jiazheng Sun Shiling Li Xijun Shao Mingxuan Fang Heng Zhang Zhiheng Zhu Xia Sun |
| author_facet | Jiazheng Sun Shiling Li Xijun Shao Mingxuan Fang Heng Zhang Zhiheng Zhu Xia Sun |
| author_sort | Jiazheng Sun |
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| description | Procymidone (PCM) is an effective, low-toxicity fungicide commonly used to control plant diseases in grains, vegetables, and fruits. Its usage has significantly increased in recent years, resulting in higher residues in vegetables. This study developed a sensitive and rapid immunoassay method utilizing a gold- and fluorescence-labeled monoclonal antibody (mAb) for detecting PCM residues in vegetable samples. Under optimal conditions, the fluorescent microsphere-labeled monoclonal antibody immunochromatographic strips achieved a limit of detection (LOD) of 1.67 ng/mL, with a visual LOD of 50 ng/mL. Intra-batch accuracy ranged from 94.98% to 103.82%, with a coefficient of variation (CV) of 1.97% to 8.26%. Inter-batch accuracy ranged from 96.16% to 102.51%, with a CV of 4.62% to 8.91%. The visual detection range of the gold nanoparticle-labeled monoclonal antibody immunochromatographic strips was 50 to 200 ng/g. The method demonstrated excellent performance in actual vegetable samples, confirming its applicability across various matrices. This dual-method approach enables rapid screening of negative samples with gold test strips, followed by accurate quantitative analysis of positive samples using fluorescent test strips, thereby enhancing efficiency and addressing diverse detection needs. Consequently, this method holds significant market potential for practical applications. |
| format | Article |
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| institution | OA Journals |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-6dc9ed4af3d04385bd1db6918b3b8ec82025-08-20T02:18:01ZengMDPI AGSensors1424-82202025-04-01258260210.3390/s25082602Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based SensorsJiazheng Sun0Shiling Li1Xijun Shao2Mingxuan Fang3Heng Zhang4Zhiheng Zhu5Xia Sun6School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaProcymidone (PCM) is an effective, low-toxicity fungicide commonly used to control plant diseases in grains, vegetables, and fruits. Its usage has significantly increased in recent years, resulting in higher residues in vegetables. This study developed a sensitive and rapid immunoassay method utilizing a gold- and fluorescence-labeled monoclonal antibody (mAb) for detecting PCM residues in vegetable samples. Under optimal conditions, the fluorescent microsphere-labeled monoclonal antibody immunochromatographic strips achieved a limit of detection (LOD) of 1.67 ng/mL, with a visual LOD of 50 ng/mL. Intra-batch accuracy ranged from 94.98% to 103.82%, with a coefficient of variation (CV) of 1.97% to 8.26%. Inter-batch accuracy ranged from 96.16% to 102.51%, with a CV of 4.62% to 8.91%. The visual detection range of the gold nanoparticle-labeled monoclonal antibody immunochromatographic strips was 50 to 200 ng/g. The method demonstrated excellent performance in actual vegetable samples, confirming its applicability across various matrices. This dual-method approach enables rapid screening of negative samples with gold test strips, followed by accurate quantitative analysis of positive samples using fluorescent test strips, thereby enhancing efficiency and addressing diverse detection needs. Consequently, this method holds significant market potential for practical applications.https://www.mdpi.com/1424-8220/25/8/2602immunochromatographic (IC) testtime-resolved fluorescenceAuNPsprocymidonevegetables |
| spellingShingle | Jiazheng Sun Shiling Li Xijun Shao Mingxuan Fang Heng Zhang Zhiheng Zhu Xia Sun Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based Sensors Sensors immunochromatographic (IC) test time-resolved fluorescence AuNPs procymidone vegetables |
| title | Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based Sensors |
| title_full | Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based Sensors |
| title_fullStr | Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based Sensors |
| title_full_unstemmed | Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based Sensors |
| title_short | Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based Sensors |
| title_sort | two methods for detecting pcm residues in vegetables based on paper based sensors |
| topic | immunochromatographic (IC) test time-resolved fluorescence AuNPs procymidone vegetables |
| url | https://www.mdpi.com/1424-8220/25/8/2602 |
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