Clinical significance and regulatory mechanism of miR-134-5p in rheumatic heart disease
Abstract Background Rheumatic heart disease (RHD) causes irreversible valvular injury and mortality globally. Easily detectable miRNAs may provide valuable perspectives into the clinical management of RHD. This study aimed to explore the role of miR-134-5p in RHD progression. Methods A total of 167...
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BMC
2025-08-01
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| Series: | BMC Cardiovascular Disorders |
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| Online Access: | https://doi.org/10.1186/s12872-025-05086-9 |
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| author | Liangqin Yang Juan Li Ju Wang Yaping Zhang Yunyun Huang |
| author_facet | Liangqin Yang Juan Li Ju Wang Yaping Zhang Yunyun Huang |
| author_sort | Liangqin Yang |
| collection | DOAJ |
| description | Abstract Background Rheumatic heart disease (RHD) causes irreversible valvular injury and mortality globally. Easily detectable miRNAs may provide valuable perspectives into the clinical management of RHD. This study aimed to explore the role of miR-134-5p in RHD progression. Methods A total of 167 subjects (80 non-RHD, 87 RHD) were included in this study. The level of miR-134-5p was evaluated by qRT-PCR, and its diagnostic value was assessed by the receiver operator characteristic (ROC) curve. The correlation between miR-134-5p and RHD severity was evaluated by correlation analysis. The association between miR-134-5p expression and RHD prognosis was analyzed by the Kaplan-Meier survival analysis and multivariate COX regression analysis. The regulatory relationship between miR-134-5p and lysine acetyltransferase 7 (KAT7) was assessed by the dual-luciferase reporter assay. In vitro cell experiment was used to assess the regulatory mechanism of miR-134-5p in lipopolysaccharide (LPS)-induced human valvular interstitial cells (hVICs). Results Upregulated miR-134-5p in RHD was correlated with RHD severity and could diagnose RHD from rheumatic fever (RF). Low miR-134-5p level was associated with a better prognosis of RHD and could serve as an independent prognostic factor for RHD. Suppression of miR-134-5p in hVICs injury could attenuate the inflammation and oxidative stress by regulating the KAT7 expression. Conclusions Upregulated miR-134-5p expression showed a diagnostic and prognostic value in RHD. MiR-134-5p may be involved in RHD by regulating the inflammation and oxidative stress in hVIC injury via targeting KAT7. |
| format | Article |
| id | doaj-art-6dc8b91d577c4e83be4a0ce499db951a |
| institution | Kabale University |
| issn | 1471-2261 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
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| series | BMC Cardiovascular Disorders |
| spelling | doaj-art-6dc8b91d577c4e83be4a0ce499db951a2025-08-24T11:06:41ZengBMCBMC Cardiovascular Disorders1471-22612025-08-0125111110.1186/s12872-025-05086-9Clinical significance and regulatory mechanism of miR-134-5p in rheumatic heart diseaseLiangqin Yang0Juan Li1Ju Wang2Yaping Zhang3Yunyun Huang4First Ward of Rheumatology and Immunology Department of Shaanxi Provincial Hospital of Integrated Traditional Chinese and Western MedicineFirst Ward of Rheumatology and Immunology Department of Shaanxi Provincial Hospital of Integrated Traditional Chinese and Western MedicineNursing Department of Shaanxi Provincial Hospital of Integrated Traditional Chinese and Western MedicineEighth Ward of Rheumatology and Immunology Department of Shaanxi Provincial Hospital of Integrated Traditional Chinese and Western MedicineDepartment of Rheumatology and Immunology, Shaanxi Provincial Hospital of Integrated Traditional Chinese and Western MedicineAbstract Background Rheumatic heart disease (RHD) causes irreversible valvular injury and mortality globally. Easily detectable miRNAs may provide valuable perspectives into the clinical management of RHD. This study aimed to explore the role of miR-134-5p in RHD progression. Methods A total of 167 subjects (80 non-RHD, 87 RHD) were included in this study. The level of miR-134-5p was evaluated by qRT-PCR, and its diagnostic value was assessed by the receiver operator characteristic (ROC) curve. The correlation between miR-134-5p and RHD severity was evaluated by correlation analysis. The association between miR-134-5p expression and RHD prognosis was analyzed by the Kaplan-Meier survival analysis and multivariate COX regression analysis. The regulatory relationship between miR-134-5p and lysine acetyltransferase 7 (KAT7) was assessed by the dual-luciferase reporter assay. In vitro cell experiment was used to assess the regulatory mechanism of miR-134-5p in lipopolysaccharide (LPS)-induced human valvular interstitial cells (hVICs). Results Upregulated miR-134-5p in RHD was correlated with RHD severity and could diagnose RHD from rheumatic fever (RF). Low miR-134-5p level was associated with a better prognosis of RHD and could serve as an independent prognostic factor for RHD. Suppression of miR-134-5p in hVICs injury could attenuate the inflammation and oxidative stress by regulating the KAT7 expression. Conclusions Upregulated miR-134-5p expression showed a diagnostic and prognostic value in RHD. MiR-134-5p may be involved in RHD by regulating the inflammation and oxidative stress in hVIC injury via targeting KAT7.https://doi.org/10.1186/s12872-025-05086-9RHDMiR-134-5pKAT7InflammationOxidative stress |
| spellingShingle | Liangqin Yang Juan Li Ju Wang Yaping Zhang Yunyun Huang Clinical significance and regulatory mechanism of miR-134-5p in rheumatic heart disease BMC Cardiovascular Disorders RHD MiR-134-5p KAT7 Inflammation Oxidative stress |
| title | Clinical significance and regulatory mechanism of miR-134-5p in rheumatic heart disease |
| title_full | Clinical significance and regulatory mechanism of miR-134-5p in rheumatic heart disease |
| title_fullStr | Clinical significance and regulatory mechanism of miR-134-5p in rheumatic heart disease |
| title_full_unstemmed | Clinical significance and regulatory mechanism of miR-134-5p in rheumatic heart disease |
| title_short | Clinical significance and regulatory mechanism of miR-134-5p in rheumatic heart disease |
| title_sort | clinical significance and regulatory mechanism of mir 134 5p in rheumatic heart disease |
| topic | RHD MiR-134-5p KAT7 Inflammation Oxidative stress |
| url | https://doi.org/10.1186/s12872-025-05086-9 |
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