Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples
PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standar...
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| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2024/4894004 |
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| author | Camilo Castellar-Mendoza María-Angélica Calderón-Peláez Jaime E. Castellanos Myriam L. Velandia-Romero Carolina Coronel-Ruiz Sigrid Camacho-Ortega Lilia J. Bernal-Cepeda Lady López-Ibarra Jhann A. Arturo Félix G. Delgado Hernando Gutierrez-Barbosa Sonia Bohorquez-Avila Johanna Madroñero Zayda L. Corredor-Rozo Sandra J. Perdomo-Lara Angela Fonseca-Benitez Eliana Calvo |
| author_facet | Camilo Castellar-Mendoza María-Angélica Calderón-Peláez Jaime E. Castellanos Myriam L. Velandia-Romero Carolina Coronel-Ruiz Sigrid Camacho-Ortega Lilia J. Bernal-Cepeda Lady López-Ibarra Jhann A. Arturo Félix G. Delgado Hernando Gutierrez-Barbosa Sonia Bohorquez-Avila Johanna Madroñero Zayda L. Corredor-Rozo Sandra J. Perdomo-Lara Angela Fonseca-Benitez Eliana Calvo |
| author_sort | Camilo Castellar-Mendoza |
| collection | DOAJ |
| description | PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral targets and ensure results, follow the infections, and favor the containment of a pandemic. Here, we present the detailed fundamentals of the qPCR technique based on fluorogenic probes and processes to develop and optimize a successful multiplex RT-qPCR test for detecting SARS-CoV-2 that could be used to diagnose COVID-19 accurately. |
| format | Article |
| id | doaj-art-6dc2e3b667fb4cde98c630bf72e71a58 |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-6dc2e3b667fb4cde98c630bf72e71a582025-02-03T05:55:27ZengWileyInternational Journal of Microbiology1687-91982024-01-01202410.1155/2024/4894004Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human SamplesCamilo Castellar-Mendoza0María-Angélica Calderón-Peláez1Jaime E. Castellanos2Myriam L. Velandia-Romero3Carolina Coronel-Ruiz4Sigrid Camacho-Ortega5Lilia J. Bernal-Cepeda6Lady López-Ibarra7Jhann A. Arturo8Félix G. Delgado9Hernando Gutierrez-Barbosa10Sonia Bohorquez-Avila11Johanna Madroñero12Zayda L. Corredor-Rozo13Sandra J. Perdomo-Lara14Angela Fonseca-Benitez15Eliana Calvo16Virology GroupVirology GroupVirology GroupVirology GroupVirology GroupVirology GroupVirology GroupVirology GroupVirology GroupVirology GroupVirology GroupVirology GroupVirology GroupUniversidad El BosqueUniversidad El BosqueUniversidad El BosqueVirology GroupPCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral targets and ensure results, follow the infections, and favor the containment of a pandemic. Here, we present the detailed fundamentals of the qPCR technique based on fluorogenic probes and processes to develop and optimize a successful multiplex RT-qPCR test for detecting SARS-CoV-2 that could be used to diagnose COVID-19 accurately.http://dx.doi.org/10.1155/2024/4894004 |
| spellingShingle | Camilo Castellar-Mendoza María-Angélica Calderón-Peláez Jaime E. Castellanos Myriam L. Velandia-Romero Carolina Coronel-Ruiz Sigrid Camacho-Ortega Lilia J. Bernal-Cepeda Lady López-Ibarra Jhann A. Arturo Félix G. Delgado Hernando Gutierrez-Barbosa Sonia Bohorquez-Avila Johanna Madroñero Zayda L. Corredor-Rozo Sandra J. Perdomo-Lara Angela Fonseca-Benitez Eliana Calvo Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples International Journal of Microbiology |
| title | Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples |
| title_full | Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples |
| title_fullStr | Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples |
| title_full_unstemmed | Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples |
| title_short | Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples |
| title_sort | development and optimization of a multiplex real time rt pcr to detect sars cov 2 in human samples |
| url | http://dx.doi.org/10.1155/2024/4894004 |
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