Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells
To date, hepatocytes derived from human-induced pluripotent stem cells (hiPSC) provide a potentially unlimited resource for clinical application and drug development. However, most hiPSC-derived hepatocyte-like cells initiated differentiation from highly purified definitive endoderm, which are insuf...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/3222427 |
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| _version_ | 1849414465184858112 |
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| author | Ying Shi Jiali Deng Xiaopu Sang Yihang Wang Fei He Xiaoni Chen Anlong Xu Fenfang Wu |
| author_facet | Ying Shi Jiali Deng Xiaopu Sang Yihang Wang Fei He Xiaoni Chen Anlong Xu Fenfang Wu |
| author_sort | Ying Shi |
| collection | DOAJ |
| description | To date, hepatocytes derived from human-induced pluripotent stem cells (hiPSC) provide a potentially unlimited resource for clinical application and drug development. However, most hiPSC-derived hepatocyte-like cells initiated differentiation from highly purified definitive endoderm, which are insufficient to accurately replicate the complex regulation of signals among multiple cells and tissues during liver organogenesis, thereby displaying an immature phenotypic and short survival time in vitro. Here, we described a protocol to achieve codifferentiation of endoderm-derived hepatocytes and mesoderm-derived nonparenchymal cells by the inclusion of BMP4 into hepatic differentiation medium, which has a beneficial effect on the hepatocyte maturation and lifespan in vitro. Our codifferentiation system suggests the important role of nonparenchymal cells in liver organogenesis. Hopefully, these hepatocytes described here provide a promising approach in the therapy of liver diseases. |
| format | Article |
| id | doaj-art-6dc261e18d1d43b8adc5500c4e46210f |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6dc261e18d1d43b8adc5500c4e46210f2025-08-20T03:33:50ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/3222427Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem CellsYing Shi0Jiali Deng1Xiaopu Sang2Yihang Wang3Fei He4Xiaoni Chen5Anlong Xu6Fenfang Wu7State Key Laboratory of BiocontrolState Key Laboratory of BiocontrolSchool of Life SciencesState Key Laboratory of BiocontrolDepartment of Central LaboratoryDepartment of Central LaboratoryState Key Laboratory of BiocontrolState Key Laboratory of BiocontrolTo date, hepatocytes derived from human-induced pluripotent stem cells (hiPSC) provide a potentially unlimited resource for clinical application and drug development. However, most hiPSC-derived hepatocyte-like cells initiated differentiation from highly purified definitive endoderm, which are insufficient to accurately replicate the complex regulation of signals among multiple cells and tissues during liver organogenesis, thereby displaying an immature phenotypic and short survival time in vitro. Here, we described a protocol to achieve codifferentiation of endoderm-derived hepatocytes and mesoderm-derived nonparenchymal cells by the inclusion of BMP4 into hepatic differentiation medium, which has a beneficial effect on the hepatocyte maturation and lifespan in vitro. Our codifferentiation system suggests the important role of nonparenchymal cells in liver organogenesis. Hopefully, these hepatocytes described here provide a promising approach in the therapy of liver diseases.http://dx.doi.org/10.1155/2022/3222427 |
| spellingShingle | Ying Shi Jiali Deng Xiaopu Sang Yihang Wang Fei He Xiaoni Chen Anlong Xu Fenfang Wu Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells Stem Cells International |
| title | Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells |
| title_full | Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells |
| title_fullStr | Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells |
| title_full_unstemmed | Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells |
| title_short | Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells |
| title_sort | generation of hepatocytes and nonparenchymal cell codifferentiation system from human induced pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2022/3222427 |
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