Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae)
Camellia nitidissima Chi (CNC), a species of golden Camellia, is well known as “the queen of camellias.” It is an ornamental, medicinal, and edible plant grown in China. In this study, we conducted a genome survey sequencing analysis and simple sequence repeat (SSR) identification of CNC using the I...
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Wiley
2022-01-01
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| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2022/5417970 |
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| author | Yu Bai Lin Ye Kang Yang Hui Wang |
| author_facet | Yu Bai Lin Ye Kang Yang Hui Wang |
| author_sort | Yu Bai |
| collection | DOAJ |
| description | Camellia nitidissima Chi (CNC), a species of golden Camellia, is well known as “the queen of camellias.” It is an ornamental, medicinal, and edible plant grown in China. In this study, we conducted a genome survey sequencing analysis and simple sequence repeat (SSR) identification of CNC using the Illumina sequencing platform. The 21-mer analysis predicted its genome size to be 2,778.82 Mb, with heterozygosity and repetition rates of 1.42% and 65.27%, respectively. The CNC genome sequences were assembled into 9,399,197 scaffolds, covering ∼2,910 Mb and an N50 of 869 base pair. Its genomic characteristics were found to be similar to those of Camellia oleifera. In addition, 1,940,616 SSRs were identified from the genome data, including mono-(61.85%), di-(28.71%), tri-(6.51%), tetra-(1.85%), penta-(0.57%), and hexanucleotide motifs (0.51%). We believe these data will provide a useful foundation for the development of novel molecular markers for CNC as well as for further whole-genome sequencing of CNC. |
| format | Article |
| id | doaj-art-6dc0293e324948738e37d945a943ffce |
| institution | Kabale University |
| issn | 1469-5073 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj-art-6dc0293e324948738e37d945a943ffce2025-02-03T06:08:44ZengWileyGenetics Research1469-50732022-01-01202210.1155/2022/5417970Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae)Yu Bai0Lin Ye1Kang Yang2Hui Wang3College of Mathematics and Information ScienceSchool of Electronic & Communication EngineeringSchool of Electronic & Communication EngineeringGuizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous RegionCamellia nitidissima Chi (CNC), a species of golden Camellia, is well known as “the queen of camellias.” It is an ornamental, medicinal, and edible plant grown in China. In this study, we conducted a genome survey sequencing analysis and simple sequence repeat (SSR) identification of CNC using the Illumina sequencing platform. The 21-mer analysis predicted its genome size to be 2,778.82 Mb, with heterozygosity and repetition rates of 1.42% and 65.27%, respectively. The CNC genome sequences were assembled into 9,399,197 scaffolds, covering ∼2,910 Mb and an N50 of 869 base pair. Its genomic characteristics were found to be similar to those of Camellia oleifera. In addition, 1,940,616 SSRs were identified from the genome data, including mono-(61.85%), di-(28.71%), tri-(6.51%), tetra-(1.85%), penta-(0.57%), and hexanucleotide motifs (0.51%). We believe these data will provide a useful foundation for the development of novel molecular markers for CNC as well as for further whole-genome sequencing of CNC.http://dx.doi.org/10.1155/2022/5417970 |
| spellingShingle | Yu Bai Lin Ye Kang Yang Hui Wang Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae) Genetics Research |
| title | Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae) |
| title_full | Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae) |
| title_fullStr | Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae) |
| title_full_unstemmed | Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae) |
| title_short | Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae) |
| title_sort | genome survey and ssr analysis of camellia nitidissima chi theaceae |
| url | http://dx.doi.org/10.1155/2022/5417970 |
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