A canine model of reversible urethral sphincter insufficiency

Abstract This study developed an animal model with internal and external urethral sphincter insufficiency by bypassing the sphincter without major damage so that the animal under study can return to normal life after the study. There is a need for a reliable, applicable, and reproducible animal mode...

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Main Authors: Pourya Shokri, Shahin Tabatabaei, Seyed Amir Mohsen Ziaee, Mir Sepehr Pedram, Mohammad Mehdi Dehghan, Nasser Shakhssalim
Format: Article
Language:English
Published: Wiley 2025-02-01
Series:Animal Models and Experimental Medicine
Subjects:
Online Access:https://doi.org/10.1002/ame2.12519
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author Pourya Shokri
Shahin Tabatabaei
Seyed Amir Mohsen Ziaee
Mir Sepehr Pedram
Mohammad Mehdi Dehghan
Nasser Shakhssalim
author_facet Pourya Shokri
Shahin Tabatabaei
Seyed Amir Mohsen Ziaee
Mir Sepehr Pedram
Mohammad Mehdi Dehghan
Nasser Shakhssalim
author_sort Pourya Shokri
collection DOAJ
description Abstract This study developed an animal model with internal and external urethral sphincter insufficiency by bypassing the sphincter without major damage so that the animal under study can return to normal life after the study. There is a need for a reliable, applicable, and reproducible animal model for studying urinary incontinency disease due to incorrect sphincter function. Seven adult male dogs were used for this study. The urethral sphincter was bypassed by inserting a catheter between the bladder neck and the distal sphincter. The animals' physical condition was closely monitored for 9 weeks, and standard urodynamic and radiologic studies were performed before and 1–2 months after surgery. The animals were killed at 9 weeks after surgery for pathological assessment. Catheter placement caused complete incontinence in the animal, with urodynamic assessments indicating that the animal was unable to control urination and radiological assessments indicating an empty bladder with a residual volume of 50 ± 10 cc. Tissue analysis did not show significant histological damage and inflammation. The study shows that by bypassing the urethral sphincter, which is a reliable and reproducible method, an animal model of urinary incontinence can be developed, which can be used in various studies such as assessing the adequacy of artificial sphincter function. The animals under study did not have any permanent defect, so they were able to return to their normal life.
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spelling doaj-art-6dbcff05bd9241eeb538bb51655f0c7a2025-08-20T02:55:10ZengWileyAnimal Models and Experimental Medicine2576-20952025-02-018237237710.1002/ame2.12519A canine model of reversible urethral sphincter insufficiencyPourya Shokri0Shahin Tabatabaei1Seyed Amir Mohsen Ziaee2Mir Sepehr Pedram3Mohammad Mehdi Dehghan4Nasser Shakhssalim5Urology and Nephrology Research Center Research Institute of Urology and Nephrology, Shahid Labbafinejad Medical Center, Urology Department, Shahid Beheshti University of Medical Sciences Tehran IranTabaLife Clinic Newton Massachusetts USAUrology and Nephrology Research Center Research Institute of Urology and Nephrology, Shahid Labbafinejad Medical Center, Urology Department, Shahid Beheshti University of Medical Sciences Tehran IranDepartment of surgery and Radiology, Faculty of Veterinary Medicine University of Tehran Tehran IranDepartment of surgery and Radiology, Faculty of Veterinary Medicine University of Tehran Tehran IranUrology and Nephrology Research Center Research Institute of Urology and Nephrology, Shahid Labbafinejad Medical Center, Urology Department, Shahid Beheshti University of Medical Sciences Tehran IranAbstract This study developed an animal model with internal and external urethral sphincter insufficiency by bypassing the sphincter without major damage so that the animal under study can return to normal life after the study. There is a need for a reliable, applicable, and reproducible animal model for studying urinary incontinency disease due to incorrect sphincter function. Seven adult male dogs were used for this study. The urethral sphincter was bypassed by inserting a catheter between the bladder neck and the distal sphincter. The animals' physical condition was closely monitored for 9 weeks, and standard urodynamic and radiologic studies were performed before and 1–2 months after surgery. The animals were killed at 9 weeks after surgery for pathological assessment. Catheter placement caused complete incontinence in the animal, with urodynamic assessments indicating that the animal was unable to control urination and radiological assessments indicating an empty bladder with a residual volume of 50 ± 10 cc. Tissue analysis did not show significant histological damage and inflammation. The study shows that by bypassing the urethral sphincter, which is a reliable and reproducible method, an animal model of urinary incontinence can be developed, which can be used in various studies such as assessing the adequacy of artificial sphincter function. The animals under study did not have any permanent defect, so they were able to return to their normal life.https://doi.org/10.1002/ame2.12519animal modelexternal urethral sphincterinternal urethral sphincterurinary incontinence
spellingShingle Pourya Shokri
Shahin Tabatabaei
Seyed Amir Mohsen Ziaee
Mir Sepehr Pedram
Mohammad Mehdi Dehghan
Nasser Shakhssalim
A canine model of reversible urethral sphincter insufficiency
Animal Models and Experimental Medicine
animal model
external urethral sphincter
internal urethral sphincter
urinary incontinence
title A canine model of reversible urethral sphincter insufficiency
title_full A canine model of reversible urethral sphincter insufficiency
title_fullStr A canine model of reversible urethral sphincter insufficiency
title_full_unstemmed A canine model of reversible urethral sphincter insufficiency
title_short A canine model of reversible urethral sphincter insufficiency
title_sort canine model of reversible urethral sphincter insufficiency
topic animal model
external urethral sphincter
internal urethral sphincter
urinary incontinence
url https://doi.org/10.1002/ame2.12519
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