<i>Kalanchoe crenata</i> Andrews (Haw.) Improves Losartan’s Antihypertensive Activity

<i>Kalanchoe crenata</i> Andrews (Haw.) Improves Losartan’s Antihypertensive Activity

Background: Cardiovascular diseases constitute one of the leading causes of morbidity and mortality worldwide. Herbal medicines represent viable alternatives to the synthetic drugs currently employed in the control of hypertension. This study aimed to isolate and identify the chemical markers of <...

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Main Authors: Pedro de Padua G. Amatto, Juliana da Silva Coppede, Carla Renata Kitanishi, Giovana Graça Braga, Thaysa Carvalho de Faria, Elen Rizzi, Suzelei de Castro França, Fernanda Basso, Adriana Aparecida Lopes, Fábio Carmona, Silvia Helena Taleb Contini, Ana Maria Soares Pereira
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Molecules
Subjects:
patuletins
hypertension
medicinal plant
systolic blood pressure
murine model
Online Access:https://www.mdpi.com/1420-3049/29/24/6010
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Summary:Background: Cardiovascular diseases constitute one of the leading causes of morbidity and mortality worldwide. Herbal medicines represent viable alternatives to the synthetic drugs currently employed in the control of hypertension. This study aimed to isolate and identify the chemical markers of <i>Kalanchoe crenata</i> and to investigate the antihypertensive and anti-matrix metalloproteinase (MMP2) activities of an aqueous extract of the leaves. Methods: The main constituents of the aqueous extract of <i>K. crenata</i> were separated by ultra-performance liquid chromatography–mass spectrometry, and their presence was identified by NMR spectroscopy. Renovascular hypertension was induced in male Wistar rats using the two-kidney one-clip method (HTN groups), while control animals (Sham groups) were submitted to Sham surgery. Six groups of 10 animals each were treated daily for eight weeks as follows: Sham 1 (carrier), Sham 2 (<i>K. crenata</i> extract), HTN.1 (carrier), HTN.2 (<i>K. crenata</i> extract), HTN 3 (losartan), and HTN 4 (<i>K. crenata</i> extract with losartan). Results: The main compounds of the extract were patuletin 3-<i>O</i>-(4″-<i>O</i>-acetyl-α-L-rhamnopyranosyl)-7-<i>O</i>-(3‴-<i>O</i>-acetyl-α-L-rhamnopyranoside) (<b>1</b>), patuletin 3-<i>O</i>-α-L-rhamnopyranosyl-7-<i>O</i>-L-rhamnopyranoside (<b>2</b>), and <i>trans</i>-caffeoyl-malic acid (<b>3</b>), with compounds <b>1</b> and <b>2</b> being chemical markers of the species. Significant reductions (<i>p</i> < 0.05) in systolic blood pressure and MMP2 (72kDa isoform) activity were observed in the HTN 4 group. Conclusions: The association of <i>K. crenata</i> extract and losartan presented in vivo effects against hypertension.
ISSN:1420-3049

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