OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in Rice
The cell surface receptor chitin elicitor receptor kinase 1 (CERK1) is a well-known component of plant immunity. OsCERK1 is involved in regulating copper (Cu) uptake in rice, though the underlying mechanisms remain elusive. In this study, we identified proteins interacting with OsCERK1 and uncovered...
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Elsevier
2025-03-01
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| Series: | Rice Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630825000137 |
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| author | Ya Chen Zhiquan Liu Linyin Yang Wu Fujie Zijian Cao Huanbin Shi Jiehua Qiu Yanjun Kou |
| author_facet | Ya Chen Zhiquan Liu Linyin Yang Wu Fujie Zijian Cao Huanbin Shi Jiehua Qiu Yanjun Kou |
| author_sort | Ya Chen |
| collection | DOAJ |
| description | The cell surface receptor chitin elicitor receptor kinase 1 (CERK1) is a well-known component of plant immunity. OsCERK1 is involved in regulating copper (Cu) uptake in rice, though the underlying mechanisms remain elusive. In this study, we identified proteins interacting with OsCERK1 and uncovered a novel heavy metal-associated domain-containing protein, OsHPP08. Our findings demonstrate that OsCERK1 phosphorylated and stabilized OsHPP08. Through structural analysis using AlphaFold, a yeast sensitivity assay of the Cu uptake-deficient yeast mutant, and Cu level measurements in oshpp08 mutants and overexpression plants (OsHPP08OE), we revealed that OsHPP08 facilitated Cu uptake. Additionally, rice seedling infection assays demonstrated that OsHPP08 positively contributed to blast resistance, with both OsCERK1 and OsHPP08 being essential for Cu-modulated blast resistance. Further analyses suggested that OsCERK1 and OsHPP08 likely enhanced blast resistance by regulating the antioxidant system and increasing H2O2 accumulation. In conclusion, OsCERK1 promoted Cu uptake by stabilizing OsHPP08, and together they contributed to Cu-modulated blast resistance, likely through the modulation of reactive oxygen species accumulation. These findings deepen our understanding of the intricate interplay between biotic and abiotic signals in rice. |
| format | Article |
| id | doaj-art-6da7a2599da5434d96aa1e684e329f5d |
| institution | OA Journals |
| issn | 1672-6308 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Rice Science |
| spelling | doaj-art-6da7a2599da5434d96aa1e684e329f5d2025-08-20T02:12:07ZengElsevierRice Science1672-63082025-03-0132220321610.1016/j.rsci.2025.02.001OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in RiceYa Chen0Zhiquan Liu1Linyin Yang2Wu Fujie3Zijian Cao4Huanbin Shi5Jiehua Qiu6Yanjun Kou7State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China; Corresponding author.The cell surface receptor chitin elicitor receptor kinase 1 (CERK1) is a well-known component of plant immunity. OsCERK1 is involved in regulating copper (Cu) uptake in rice, though the underlying mechanisms remain elusive. In this study, we identified proteins interacting with OsCERK1 and uncovered a novel heavy metal-associated domain-containing protein, OsHPP08. Our findings demonstrate that OsCERK1 phosphorylated and stabilized OsHPP08. Through structural analysis using AlphaFold, a yeast sensitivity assay of the Cu uptake-deficient yeast mutant, and Cu level measurements in oshpp08 mutants and overexpression plants (OsHPP08OE), we revealed that OsHPP08 facilitated Cu uptake. Additionally, rice seedling infection assays demonstrated that OsHPP08 positively contributed to blast resistance, with both OsCERK1 and OsHPP08 being essential for Cu-modulated blast resistance. Further analyses suggested that OsCERK1 and OsHPP08 likely enhanced blast resistance by regulating the antioxidant system and increasing H2O2 accumulation. In conclusion, OsCERK1 promoted Cu uptake by stabilizing OsHPP08, and together they contributed to Cu-modulated blast resistance, likely through the modulation of reactive oxygen species accumulation. These findings deepen our understanding of the intricate interplay between biotic and abiotic signals in rice.http://www.sciencedirect.com/science/article/pii/S1672630825000137Oryza sativacopperMagnaporthe oryzaeheavy metal ATPase domainblast resistancereactive oxygen species |
| spellingShingle | Ya Chen Zhiquan Liu Linyin Yang Wu Fujie Zijian Cao Huanbin Shi Jiehua Qiu Yanjun Kou OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in Rice Rice Science Oryza sativa copper Magnaporthe oryzae heavy metal ATPase domain blast resistance reactive oxygen species |
| title | OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in Rice |
| title_full | OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in Rice |
| title_fullStr | OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in Rice |
| title_full_unstemmed | OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in Rice |
| title_short | OsCERK1 Interacts with OsHPP08 to Regulate Copper Uptake and Blast Resistance in Rice |
| title_sort | oscerk1 interacts with oshpp08 to regulate copper uptake and blast resistance in rice |
| topic | Oryza sativa copper Magnaporthe oryzae heavy metal ATPase domain blast resistance reactive oxygen species |
| url | http://www.sciencedirect.com/science/article/pii/S1672630825000137 |
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