MicroRNA expression and GPX-1 genetic polymorphisms in patients undergoing hemodialysis: Implications for oxidative stress in a cross-sectional study
MicroRNAs (miRNAs) are crucial regulators of gene expression and have been implicated in renal pathology. The glutathione peroxidase-1 (GPX-1) gene, particularly the rs1050450 single nucleotide polymorphism (SNP), may modulate oxidative stress responses in hemodialysis patients. This study examines...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
National Kidney Foundation of Ukraine
2025-02-01
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| Series: | Український Журнал Нефрології та Діалізу |
| Subjects: | |
| Online Access: | https://ukrjnd.com.ua/index.php/journal/article/view/912 |
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| Summary: | MicroRNAs (miRNAs) are crucial regulators of gene expression and have been implicated in renal pathology. The glutathione peroxidase-1 (GPX-1) gene, particularly the rs1050450 single nucleotide polymorphism (SNP), may modulate oxidative stress responses in hemodialysis patients. This study examines the interplay between miRNA expression, oxidative stress, and GPX-1 genetic polymorphisms in hemodialysis patients.
Methods. A total of 60 hemodialysis patients and 40 healthy controls were recruited. Blood samples were collected and analyzed for miRNA expression (miRNA-143, miRNA-145, miRNA-155, and miRNA-192) using RT-qPCR. GPX-1 rs1050450 polymorphism was detected via conventional PCR and sequencing. Oxidative stress biomarkers, malondialdehyde (MDA), and 8-hydroxy-deoxyguanosine (8-OHDG) were measured using ELISA. Statistical analyses included Pearson correlation and chi-square tests, with significance set at p < 0.05.
Results. Hemodialysis patients exhibited significantly upregulated miRNA-143 (4.31-fold) and miRNA-155 (1.79-fold) compared to controls (p = 0.04). miRNA-192 expression was downregulated (0.27-fold), though not statistically significant (p = 0.12). Pearson correlation analysis showed a significant positive correlation between oxidative stress markers (8-OHDG, MDA) and miRNA-145, miRNA-155, and miRNA-192 (p ≤ 0.001). Genetic analysis of GPX-1 rs1050450 revealed CC, CT, and TT genotypes in hemodialysis patients, with Hardy-Weinberg equilibrium maintained (p = 0.46 for patients, p = 0.8 for controls).
Conclusions. The differential expression of miRNAs in hemodialysis patients suggests a role in oxidative stress regulation and renal disease progression. Upregulation of miRNA-143, miRNA-145, and miRNA-155 may contribute to inflammatory and fibrotic pathways, while miRNA-192 downregulation may reflect altered renal function. The GPX-1 rs1050450 polymorphism may modulate oxidative stress responses in these patients. Further studies are needed to explore the therapeutic potential of miRNA-based interventions in CKD management.
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| ISSN: | 2304-0238 2616-7352 |